Evaluation and Genome Mining of Bacillus stercoris Isolate B.PNR1 as Potential Agent for Fusarium Wilt Control and Growth Promotion of Tomato.

IF 1.8 3区 农林科学 Q2 PLANT SCIENCES Plant Pathology Journal Pub Date : 2023-10-01 DOI:10.5423/PPJ.OA.01.2023.0018
Rattana Pengproh, Thanwanit Thanyasiriwat, Kusavadee Sangdee, Juthaporn Saengprajak, Praphat Kawicha, Aphidech Sangdee
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Abstract

Recently, strategies for controlling Fusarium oxysporum f. sp. lycopersici (Fol), the causal agent of Fusarium wilt of tomato, focus on using effective biocontrol agents. In this study, an analysis of the biocontrol and plant growth promoting (PGP) attributes of 11 isolates of loamy soil Bacillus spp. has been conducted. Among them, the isolates B.PNR1 and B.PNR2 inhibited the mycelial growth of Fol by inducing abnormal fungal cell wall structures and cell wall collapse. Moreover, broad-spectrum activity against four other plant pathogenic fungi, F. oxysporum f. sp. cubense race 1 (Foc), Sclerotium rolfsii, Colletotrichum musae, and C. gloeosporioides were noted for these isolates. These two Bacillus isolates produced indole acetic acid, phosphate solubilization enzymes, and amylolytic and cellulolytic enzymes. In the pot experiment, the culture filtrate from B.PNR1 showed greater inhibition of the fungal pathogens and significantly promoted the growth of tomato plants more than those of the other treatments. Isolate B.PNR1, the best biocontrol and PGP, was identified as Bacillus stercoris by its 16S rRNA gene sequence and whole genome sequencing analysis (WGS). The WGS, through genome mining, confirmed that the B.PNR1 genome contained genes/gene cluster of a nonribosomal peptide synthetase/polyketide synthase, such as fengycin, surfactin, bacillaene, subtilosin A, bacilysin, and bacillibactin, which are involved in antagonistic and PGP activities. Therefore, our finding demonstrates the effectiveness of B. stercoris strain B.PNR1 as an antagonist and for plant growth promotion, highlighting the use of this microorganism as a biocontrol agent against the Fusarium wilt pathogen and PGP abilities in tomatoes.

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stercoris芽孢杆菌分离株B.PNR1作为番茄枯萎病防治和生长促进剂的评价和基因组挖掘。
近年来,番茄枯萎病的病原尖孢镰刀菌(Fusarium oxysporum f.sp.lycopersici,Fol)的防治策略主要集中在使用有效的生物防治剂上。本研究对11株泥沼土壤芽孢杆菌的生防和促生长特性进行了分析。其中,分离株B.PNR1和B.PNR2通过诱导真菌细胞壁结构异常和细胞壁塌陷来抑制Fol的菌丝生长。此外,这些分离株对其他四种植物病原真菌,即尖孢镰刀菌(F.oxysporum F.sp.cubense race 1(Foc))、硬核菌(Sclerotium rolfsii)、炭疽菌(Colletotrichum musae)和球孢菌(C.gloeosporioides)具有广谱活性。这两个芽孢杆菌分离株产生吲哚乙酸、磷酸盐溶解酶、淀粉分解酶和纤维素分解酶。在盆栽试验中,B.PNR1培养滤液对真菌病原体的抑制作用更强,对番茄植株生长的促进作用也比其他处理更强。通过16S rRNA基因序列和全基因组测序分析(WGS),鉴定出最佳生防菌和PGP菌株B.PNR1为斯特氏芽孢杆菌。WGS通过基因组挖掘证实,B.PNR1基因组包含非核糖体肽合成酶/聚酮合酶的基因/基因簇,如丰霉素、表面活性素、杆菌素、枯草杆菌素a、杆菌素和杆菌肌动蛋白,它们参与拮抗和PGP活性。因此,我们的发现证明了B.stercoris菌株B.PNR1作为拮抗剂和促进植物生长的有效性,强调了该微生物作为生物防治剂对枯萎病病原体的作用和番茄中PGP的能力。
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来源期刊
Plant Pathology Journal
Plant Pathology Journal 生物-植物科学
CiteScore
4.90
自引率
4.30%
发文量
71
审稿时长
12 months
期刊介绍: Information not localized
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