Cytotoxic phenazine and antiallergic phenoxazine alkaloids from an arctic Nocardiopsis dassonvillei SCSIO 502F

IF 4.8 3区化学 Q1 CHEMISTRY, MEDICINAL Natural Products and Bioprospecting Pub Date : 2023-10-18 DOI:10.1007/s13659-023-00408-w

Yue Song, Qi-Yang Li, Meng-Jing Cong, Xiao-Yan Pang, Bo Chen, Yong-Hong Liu, Li Liao, Jun-Feng Wang

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Abstract

Microbes well-adapted to the Arctic Ocean are promising for producing novel compounds, due to their fancy strategies for adaptation and being under-investigated. Two new phenazine alkaloids (1 and 2) and one new phenoxazine (3) were isolated from Nocardiopsis dassonvillei 502F, a strain originally isolated from Arctic deep-sea sediments. AntiSMASH analysis of the genome of Nocardiopsis dassonvillei 502F revealed the presence of 16 putative biosynthetic gene clusters (BGCs), including a phenazine BGC. Most of the isolated compounds were evaluated for their antibacterial, antiallergic, and cytotoxic activities. Among them, compounds 4 and 5 exhibited potent in vitro cytotoxic activities against osteosarcoma cell line 143B with IC₅₀ values 0.16 and 20.0 μM, respectively. Besides, the results of antiallergic activities of compounds 6–8 exhibited inhibitory activities with IC₅₀ values of 10.88 ± 3.05, 38.88 ± 3.29, and 2.44 ± 0.17 μg/mL, respectively (IC₅₀ 91.6 μM for the positive control loratadine).

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北极诺卡氏病SCSIO502F的细胞毒性吩嗪和抗变态反应吩恶嗪生物碱。

适应北冰洋的微生物很有可能产生新的化合物，因为它们具有奇特的适应策略，而且正在研究中。从北极深海沉积物中分离得到2个新的吩嗪生物碱（1和2）和1个新的苯恶嗪（3）。对Nocardiosis dassonvillei 502F基因组的抗SMASH分析显示存在16个假定的生物合成基因簇（BGCs），包括吩嗪BGCs。大多数分离的化合物都被评估为具有抗菌、抗过敏和细胞毒性活性。其中，化合物4和5对骨肉瘤细胞系143B表现出强大的体外细胞毒性活性，IC50值分别为0.16和20.0μM。此外，化合物6-8的抗变态反应活性结果显示出抑制活性，IC50值为10.88 ± 3.05、38.88 ± 3.29和2.44 ± 0.17μg/mL（阳性对照氯雷他定的IC50为91.6μM）。

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