R-loops act as regulatory switches modulating transcription of COLD-responsive genes in rice

IF 8.1 1区生物学 Q1 PLANT SCIENCES New Phytologist Pub Date : 2023-10-17 DOI:10.1111/nph.19315

Zexue He, Mengqi Li, Xiucai Pan, Yulian Peng, Yining Shi, Qi Han, Manli Shi, Linwei She, Gennadii Borovskii, Xiaojun Chen, Xiaofeng Gu, Xuejiao Cheng, Wenli Zhang

引用次数: 0

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R-环作为调控开关，调节水稻COLD反应基因的转录。

感冒是一种主要的自然发生的压力，通常会导致复杂的症状和严重的作物产量损失。R环在植物的各种细胞过程中发挥作用，包括发育和应激反应。然而，在水稻（Oryza sativa L.）等易感冷作物中，R环在冷反应中的作用在很大程度上是未知的。我们在有或没有冷处理的水稻中进行了DRIP-Seq和其他组学数据（RNA-Seq、DNase-Seq和ChIP-Seq）。COLD治疗导致整个基因组的R环重编程。COLD偏置的R-环具有更高的GC含量和新的结合不同转录因子（TF）的基序。此外，R-环可以直接/间接调节COLD反应基因亚群的转录，这可以由R-环重叠的TF中心或顺式调节元件相关的调节网络和lncRNA介导，解释c。 60%的COLD在水稻中诱导差异基因的表达，这与在拟南芥中的发现不同。我们验证了两个R环基因座在调节两个单独的COLD反应基因表达中具有对比（阴性/阳性）作用，作为增强COLD抗性的潜在靶标。我们的研究提供了详细的证据，表明R环重编程在COLD反应中的功能，并为育种耐冷性增强的水稻品种提供了一些潜在的R环基因座，用于遗传和表观遗传学操作。

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来源期刊

New Phytologist 生物-植物科学

自引率

5.30%

发文量

728

期刊介绍： New Phytologist is an international electronic journal published 24 times a year. It is owned by the New Phytologist Foundation, a non-profit-making charitable organization dedicated to promoting plant science. The journal publishes excellent, novel, rigorous, and timely research and scholarship in plant science and its applications. The articles cover topics in five sections: Physiology & Development, Environment, Interaction, Evolution, and Transformative Plant Biotechnology. These sections encompass intracellular processes, global environmental change, and encourage cross-disciplinary approaches. The journal recognizes the use of techniques from molecular and cell biology, functional genomics, modeling, and system-based approaches in plant science. Abstracting and Indexing Information for New Phytologist includes Academic Search, AgBiotech News & Information, Agroforestry Abstracts, Biochemistry & Biophysics Citation Index, Botanical Pesticides, CAB Abstracts®, Environment Index, Global Health, and Plant Breeding Abstracts, and others.