WntA and Wnt4 during the regeneration of internal organs in the holothurian Eupentacta fraudatrix

IF 2.4 4区生物学 Q2 DEVELOPMENTAL BIOLOGY genesis Pub Date : 2023-10-17 DOI:10.1002/dvg.23562

A. S. Girich

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Abstract

Background

Over the past few years, it has been established that wnt genes are involved in the regenerative processes of holothurians. The wnt4 gene was identified as one of the most active genes in Eupentacta fraudatrix regeneration using differential gene expression analysis and qPCR of individual genes. Also, the wntA gene was found in holothurians, which is present only in invertebrates and can perform unique functions.

Results

In this regard, both these genes and proteins were studied in this work. During regeneration, the Wnt4 protein is found in the cells of the coelomic and ambulacral epithelium, retractor muscles, and radial nerves. Single cells with this protein are also found in the connective tissue of the developing aquapharyngeal bulb and in the hypoderm of the body wall. Cells with WntA are found exclusively in the hypoderm of the body wall.

Conclusion

We assume that both genes are involved in regeneration, but Wnt4 coordinates the formation of the epithelial tissue structure, while WntA maintains the state of the intercellular substance of the body wall.

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WntA和Wnt4。

背景：在过去的几年里，人们已经确定wnt基因参与了全息虫的再生过程。通过对各个基因的差异基因表达分析和qPCR，确定wnt4基因是真五面虫再生中最活跃的基因之一。此外，wntA基因是在全息虫中发现的，它只存在于无脊椎动物中，可以发挥独特的功能。结果：在这方面，这项工作对这些基因和蛋白质都进行了研究。在再生过程中，Wnt4蛋白存在于体腔上皮和救护车上皮、牵开器肌肉和桡神经的细胞中。在发育中的咽球的结缔组织和体壁的皮下组织中也发现了含有这种蛋白质的单细胞。WntA细胞只存在于体壁的皮下。结论：我们认为这两个基因都参与了再生，但Wnt4协调了上皮组织结构的形成，而WntA维持了体壁细胞间物质的状态。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

genesis 生物-发育生物学

CiteScore

3.60

自引率

0.00%

发文量

审稿时长

6-12 weeks

期刊介绍： As of January 2000, Developmental Genetics was renamed and relaunched as genesis: The Journal of Genetics and Development, with a new scope and Editorial Board. The journal focuses on work that addresses the genetics of development and the fundamental mechanisms of embryological processes in animals and plants. With increased awareness of the interplay between genetics and evolutionary change, particularly during developmental processes, we encourage submission of manuscripts from all ecological niches. The expanded numbers of genomes for which sequencing is being completed will facilitate genetic and genomic examination of developmental issues, even if the model system does not fit the “classical genetic” mold. Therefore, we encourage submission of manuscripts from all species. Other areas of particular interest include: 1) the roles of epigenetics, microRNAs and environment on developmental processes; 2) genome-wide studies; 3) novel imaging techniques for the study of gene expression and cellular function; 4) comparative genetics and genomics and 5) animal models of human genetic and developmental disorders. genesis presents reviews, full research articles, short research letters, and state-of-the-art technology reports that promote an understanding of the function of genes and the roles they play in complex developmental processes.