Cytobrush and cotton swab as sampling tools for molecular diagnosis of female genital schistosomiasis in the uterine cervix

Doudou Sow , Coumba Nar Ndiour , Ousmane Thiam , Magatte Ndiaye , Pape Ndiole Diagne , Souleymane Doucouré , Bruno Senghor , Oumar Gaye , Cheikh Sokhna , Babacar Faye
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Abstract

Female genital schistosomiasis (FGS) caused by Schistosoma haematobium is a neglected chronic parasitic disease. Diagnosis relies mainly on a colposcopy, which reveals non-specific lesions. This study aimed to assess the performance of two sampling methods for the molecular diagnosis of FGS in the uterine cervix. We conducted a descriptive cross-sectional study in women of reproductive age in Saint Louis, Senegal, who presented for cervical cancer screening. Cotton swab and cytobrush samples were collected from the cervix and examined by real-time PCR. The PCR results obtained using the cotton swabs were compared with those obtained using cytobrush. Of the 189 women recruited, 56 (30%) were found to be positive for S. haematobium infection via real-time PCR. Women aged 40–54 years were predominantly infected (45%) followed by those aged 25–39 years (36%). Numerically more PCR-positive specimens were identified using cytobrush sampling. Of the 89 women who underwent both cytobrush and cotton swab sampling, 27 were PCR-positive in the cytobrush sampling vs 4 in the swab sampling. The mean Ct-value was 31.0 ± 3.8 for cytobrush-based PCR vs 30.0 ± 4.4 for swab-based PCR. The results confirm that real-time PCR can detect Schistosoma haematobium DNA in the uterine cervix. The next step will be to compare PCR with the other diagnostic methods of FGS.

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细胞刷和棉签作为宫颈女性生殖器血吸虫病分子诊断的采样工具。
由血吸虫引起的女性生殖器血吸虫病是一种被忽视的慢性寄生虫病。诊断主要依靠阴道镜检查,它可以显示非特异性病变。本研究旨在评估两种采样方法对子宫颈FGS分子诊断的性能。我们对塞内加尔圣路易斯的育龄妇女进行了一项描述性横断面研究,这些妇女参加了癌症宫颈筛查。从宫颈采集棉签和细胞刷样本,并通过实时PCR进行检查。将使用棉签获得的PCR结果与使用细胞刷获得的结果进行比较。在招募的189名女性中,通过实时PCR发现56人(30%)对埃及血吸虫感染呈阳性。40-54岁的女性主要受感染(45%),其次是25-39岁的女性(36%)。使用细胞刷取样鉴定出更多的PCR阳性标本。在89名同时接受细胞刷和棉签采样的女性中,27名在细胞刷采样中呈PCR阳性,而4名在棉签采样中呈阳性。基于刷细胞的PCR的平均Ct值为31.0±3.8,而基于拭子的PCR为30.0±4.4。结果证实实时聚合酶链式反应可以检测子宫颈中的血吸虫DNA。下一步是将PCR与FGS的其他诊断方法进行比较。
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