Cytobrush and cotton swab as sampling tools for molecular diagnosis of female genital schistosomiasis in the uterine cervix

Doudou Sow , Coumba Nar Ndiour , Ousmane Thiam , Magatte Ndiaye , Pape Ndiole Diagne , Souleymane Doucouré , Bruno Senghor , Oumar Gaye , Cheikh Sokhna , Babacar Faye
{"title":"Cytobrush and cotton swab as sampling tools for molecular diagnosis of female genital schistosomiasis in the uterine cervix","authors":"Doudou Sow ,&nbsp;Coumba Nar Ndiour ,&nbsp;Ousmane Thiam ,&nbsp;Magatte Ndiaye ,&nbsp;Pape Ndiole Diagne ,&nbsp;Souleymane Doucouré ,&nbsp;Bruno Senghor ,&nbsp;Oumar Gaye ,&nbsp;Cheikh Sokhna ,&nbsp;Babacar Faye","doi":"10.1016/j.crpvbd.2023.100143","DOIUrl":null,"url":null,"abstract":"<div><p>Female genital schistosomiasis (FGS) caused by <em>Schistosoma haematobium</em> is a neglected chronic parasitic disease. Diagnosis relies mainly on a colposcopy, which reveals non-specific lesions. This study aimed to assess the performance of two sampling methods for the molecular diagnosis of FGS in the uterine cervix. We conducted a descriptive cross-sectional study in women of reproductive age in Saint Louis, Senegal, who presented for cervical cancer screening. Cotton swab and cytobrush samples were collected from the cervix and examined by real-time PCR. The PCR results obtained using the cotton swabs were compared with those obtained using cytobrush. Of the 189 women recruited, 56 (30%) were found to be positive for <em>S. haematobium</em> infection <em>via</em> real-time PCR. Women aged 40–54 years were predominantly infected (45%) followed by those aged 25–39 years (36%). Numerically more PCR-positive specimens were identified using cytobrush sampling. Of the 89 women who underwent both cytobrush and cotton swab sampling, 27 were PCR-positive in the cytobrush sampling <em>vs</em> 4 in the swab sampling. The mean Ct-value was 31.0 ± 3.8 for cytobrush-based PCR <em>vs</em> 30.0 ± 4.4 for swab-based PCR. The results confirm that real-time PCR can detect <em>Schistosoma haematobium</em> DNA in the uterine cervix. The next step will be to compare PCR with the other diagnostic methods of FGS.</p></div>","PeriodicalId":94311,"journal":{"name":"Current research in parasitology & vector-borne diseases","volume":"4 ","pages":"Article 100143"},"PeriodicalIF":1.7000,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/c7/47/main.PMC10570942.pdf","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Current research in parasitology & vector-borne diseases","FirstCategoryId":"1085","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S2667114X23000316","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q3","JCRName":"PARASITOLOGY","Score":null,"Total":0}
引用次数: 0

Abstract

Female genital schistosomiasis (FGS) caused by Schistosoma haematobium is a neglected chronic parasitic disease. Diagnosis relies mainly on a colposcopy, which reveals non-specific lesions. This study aimed to assess the performance of two sampling methods for the molecular diagnosis of FGS in the uterine cervix. We conducted a descriptive cross-sectional study in women of reproductive age in Saint Louis, Senegal, who presented for cervical cancer screening. Cotton swab and cytobrush samples were collected from the cervix and examined by real-time PCR. The PCR results obtained using the cotton swabs were compared with those obtained using cytobrush. Of the 189 women recruited, 56 (30%) were found to be positive for S. haematobium infection via real-time PCR. Women aged 40–54 years were predominantly infected (45%) followed by those aged 25–39 years (36%). Numerically more PCR-positive specimens were identified using cytobrush sampling. Of the 89 women who underwent both cytobrush and cotton swab sampling, 27 were PCR-positive in the cytobrush sampling vs 4 in the swab sampling. The mean Ct-value was 31.0 ± 3.8 for cytobrush-based PCR vs 30.0 ± 4.4 for swab-based PCR. The results confirm that real-time PCR can detect Schistosoma haematobium DNA in the uterine cervix. The next step will be to compare PCR with the other diagnostic methods of FGS.

Abstract Image

查看原文
分享 分享
微信好友 朋友圈 QQ好友 复制链接
本刊更多论文
细胞刷和棉签作为宫颈女性生殖器血吸虫病分子诊断的采样工具。
由血吸虫引起的女性生殖器血吸虫病是一种被忽视的慢性寄生虫病。诊断主要依靠阴道镜检查,它可以显示非特异性病变。本研究旨在评估两种采样方法对子宫颈FGS分子诊断的性能。我们对塞内加尔圣路易斯的育龄妇女进行了一项描述性横断面研究,这些妇女参加了癌症宫颈筛查。从宫颈采集棉签和细胞刷样本,并通过实时PCR进行检查。将使用棉签获得的PCR结果与使用细胞刷获得的结果进行比较。在招募的189名女性中,通过实时PCR发现56人(30%)对埃及血吸虫感染呈阳性。40-54岁的女性主要受感染(45%),其次是25-39岁的女性(36%)。使用细胞刷取样鉴定出更多的PCR阳性标本。在89名同时接受细胞刷和棉签采样的女性中,27名在细胞刷采样中呈PCR阳性,而4名在棉签采样中呈阳性。基于刷细胞的PCR的平均Ct值为31.0±3.8,而基于拭子的PCR为30.0±4.4。结果证实实时聚合酶链式反应可以检测子宫颈中的血吸虫DNA。下一步是将PCR与FGS的其他诊断方法进行比较。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
求助全文
约1分钟内获得全文 去求助
来源期刊
CiteScore
3.60
自引率
0.00%
发文量
0
期刊最新文献
Editorial Board Table of Contents An abortion storm in a goat farm in the Northeast Region of Brazil was caused by the atypical Toxoplasma gondii genotype #13 Helminth fauna of the black goby Gobius niger L. (Gobiiformes: Gobiidae) from the Finnish Archipelago, Baltic Sea: Molecular and morphological data A case of abnormal swimming patterns in juvenile Oblada melanura naturally infected with Philometra obladae (Nematoda: Philometridae) in the Tyrrhenian Sea off Sicily, Italy
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
现在去查看 取消
×
提示
确定
0
微信
客服QQ
Book学术公众号 扫码关注我们
反馈
×
意见反馈
请填写您的意见或建议
请填写您的手机或邮箱
已复制链接
已复制链接
快去分享给好友吧!
我知道了
×
扫码分享
扫码分享
Book学术官方微信
Book学术文献互助
Book学术文献互助群
群 号:481959085
Book学术
文献互助 智能选刊 最新文献 互助须知 联系我们:info@booksci.cn
Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。
Copyright © 2023 Book学术 All rights reserved.
ghs 京公网安备 11010802042870号 京ICP备2023020795号-1