Shaoli Liu, Yi Zheng, Yimiao Ma, Abid Sarwar, Xiao Zhao, Tianqi Luo, Zhennai Yang
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引用次数: 0
Abstract
Heavy metals are a growing threat to human health due to the resulting damage to the ecology; the removal of heavy metals by lactic acid bacteria (LAB) has been a focus of many studies. In this study, 10 LAB strains were evaluated for their ability to absorb and tolerate lead. Lactobacillus plantarum YW11 was found to possess the strongest ability of lead absorbing and tolerance, with the rate of absorption as high as 99.9% and the minimum inhibitory concentration of lead on YW11 higher than 1000 mg/L. Based on the isobaric tags for relative and absolute quantitation (iTRAQ) proteomics analysis of YW11, a total of 2009 proteins were identified both in the lead-treated strain and the control without the lead treatment. Among these proteins, 44 different proteins were identified. The abundance of 25 proteins increased significantly, and 19 proteins decreased significantly in the treatment group. These significantly differential abundant proteins are involved in the biological processes of amino acid and lipid metabolism, energy metabolism, cell wall biosynthesis, and substance transport. This study contributed further understanding of the molecular mechanism of L. plantarum in the binding and removal of lead to explore its potential application in counteracting heavy metal pollution of environment, food, and other fields.
重金属对生态环境的破坏对人类健康的威胁日益严重;乳酸菌对重金属的去除一直是研究的热点。本研究对10株乳酸菌对铅的吸收和耐受能力进行了评价。植物乳杆菌YW11对铅的吸收和耐受性最强，对铅的吸收率高达99.9%，对铅的最小抑制浓度高于1000 mg/L。利用iTRAQ (isoobic tags for relative and absolute quantitation)对YW11进行蛋白质组学分析，在铅处理菌株和未铅处理的对照组中共鉴定出2009个蛋白。在这些蛋白质中，鉴定出44种不同的蛋白质。治疗组有25种蛋白丰度显著升高，19种蛋白丰度显著降低。这些显著差异丰富的蛋白质参与氨基酸和脂质代谢、能量代谢、细胞壁生物合成和物质运输等生物过程。本研究有助于进一步了解植物乳杆菌结合和去除铅的分子机制，探索其在对抗环境、食品等领域重金属污染方面的潜在应用。
期刊介绍：
ournal of Supply Chain Management
Mission:
The mission of the Journal of Supply Chain Management (JSCM) is to be the premier choice among supply chain management scholars from various disciplines. It aims to attract high-quality, impactful behavioral research that focuses on theory building and employs rigorous empirical methodologies.
Article Requirements:
An article published in JSCM must make a significant contribution to supply chain management theory. This contribution can be achieved through either an inductive, theory-building process or a deductive, theory-testing approach. This contribution may manifest in various ways, such as falsification of conventional understanding, theory-building through conceptual development, inductive or qualitative research, initial empirical testing of a theory, theoretically-based meta-analysis, or constructive replication that clarifies the boundaries or range of a theory.
Theoretical Contribution:
Manuscripts should explicitly convey the theoretical contribution relative to the existing supply chain management literature, and when appropriate, to the literature outside of supply chain management (e.g., management theory, psychology, economics).
Empirical Contribution:
Manuscripts published in JSCM must also provide strong empirical contributions. While conceptual manuscripts are welcomed, they must significantly advance theory in the field of supply chain management and be firmly grounded in existing theory and relevant literature. For empirical manuscripts, authors must adequately assess validity, which is essential for empirical research, whether quantitative or qualitative.
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