Sugar beet cells cellular and extracellular events taking place in response to drought and salinity

IF 1.1 4区 生物学 Q3 PLANT SCIENCES Acta Botanica Croatica Pub Date : 2023-05-19 DOI:10.37427/botcro-2023-008
D. Pavoković, A. Horvatić, I. Tomljanović, B. Balen, M. Krsnik-Rasol
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Abstract

Salt and drought stress are important abiotic factors that negatively affect plant growth and yield. To understand how these stress factors affect metabolism at the cellular level, we analyzed cation concentrations and expression of cellular and extracellular proteins, as well as their functions and types. Cells of the industrially important halophyte sugar beet were exposed to 300 mM NaCl and 600 mM mannitol as stressors in modified Gamborg B5 liquid medium (PG0). Severe stress altered the intracellular concentrations of the most measured cations. The cellular proteome revealed that both stressors provoked significant differential regulation of 110 cellular proteins. About 80% of the identified proteins were classified in metabolism, energy, or cell rescue, defense and virulence categories. We identified several novel proteins that respond to stress, including a member of the bZIP family of transcription factors, a member of the glycine-rich RNA-binding proteins, and the K+ channel beta subunit. Among extracellular proteins we found previously unreported stress-responsive proteins, a beta-xylosidase and an isoform of chitinase. The obtained results indicate that salt and drought stress disturbed the concentrations of cellular cations and the affected expression of cellular and extracellular proteins in sugar beet cells.
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甜菜细胞对干旱和盐度的反应是细胞和细胞外事件
盐和干旱胁迫是影响植物生长和产量的重要非生物因素。为了了解这些应激因素如何在细胞水平上影响代谢,我们分析了阳离子浓度、细胞和细胞外蛋白质的表达,以及它们的功能和类型。在改良的Gamborg B5液体培养基(PG0)中将工业上重要的盐生植物甜菜的细胞暴露于300mM NaCl和600mM甘露醇作为应激源。严重的应激改变了细胞内测得最多的阳离子的浓度。细胞蛋白质组显示,两种应激源都引起了110种细胞蛋白质的显著差异调节。大约80%的已鉴定蛋白质被分为代谢、能量或细胞拯救、防御和毒力类别。我们鉴定了几种对应激有反应的新蛋白质,包括bZIP转录因子家族的一员、富含甘氨酸的RNA结合蛋白的一员和K+通道β亚基。在细胞外蛋白中,我们发现了以前未报道的应激反应蛋白,一种β-木糖苷酶和一种几丁质酶异构体。结果表明,盐和干旱胁迫干扰了甜菜细胞中细胞阳离子的浓度,并影响了细胞和细胞外蛋白的表达。
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来源期刊
Acta Botanica Croatica
Acta Botanica Croatica PLANT SCIENCES-
CiteScore
2.50
自引率
0.00%
发文量
34
审稿时长
>12 weeks
期刊介绍: The interest of the journal is field (terrestrial and aquatic) and experimental botany (including microorganisms, plant viruses, bacteria, unicellular algae), from subcellular level to ecosystems. The attention of the Journal is aimed to the research of karstic areas of the southern Europe, karstic waters and the Adriatic Sea (Mediterranean).
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