Pub Date : 2023-11-20DOI: 10.37427/botcro-2024-003
Ibrahim Abd el-wahab Mohamed, Mona Hassan, Mostafa Aboulela
The holo-parasitic weed Orobanche crenata Forrsk. is a threat to economically important legumes and vegetables in Mediterranean countries, including Egypt. The crenate broomrape attacks several wild and cultivated plant species, and documentation of new hosts of the parasite is always required. To the best of our knowledge, this study is the first report of parasitism of the crenate broomrape on two ornamental species, Arctotis fastuosa Jacq. and Callistephus chinensis (L.) Nees. (Asteraceae). We also recorded for the first time its parasitism on the wild weeds (Ammi majus L., Lactuca serriola L., and Melilotus indicus (L.) All.) and the cultivated plant species (Carthamus tinctorius L. and Tropaeolum majus L.) from Egypt. The occurrence of O. crenata parasitism was confirmed by the attachment of its haustoria to the roots of host plants. The incidence of crenate broomrape disease was estimated for the seven species. The study also provides a morphological description of the polymorphic O. crenata on the samples from Egypt and determines the most useful characteristics for its easier identification in the field.
{"title":"New hosts and diagnostic characteristics of Orobanche crenata (Orobanchaceae) in Egypt","authors":"Ibrahim Abd el-wahab Mohamed, Mona Hassan, Mostafa Aboulela","doi":"10.37427/botcro-2024-003","DOIUrl":"https://doi.org/10.37427/botcro-2024-003","url":null,"abstract":"The holo-parasitic weed Orobanche crenata Forrsk. is a threat to economically important legumes and vegetables in Mediterranean countries, including Egypt. The crenate broomrape attacks several wild and cultivated plant species, and documentation of new hosts of the parasite is always required. To the best of our knowledge, this study is the first report of parasitism of the crenate broomrape on two ornamental species, Arctotis fastuosa Jacq. and Callistephus chinensis (L.) Nees. (Asteraceae). We also recorded for the first time its parasitism on the wild weeds (Ammi majus L., Lactuca serriola L., and Melilotus indicus (L.) All.) and the cultivated plant species (Carthamus tinctorius L. and Tropaeolum majus L.) from Egypt. The occurrence of O. crenata parasitism was confirmed by the attachment of its haustoria to the roots of host plants. The incidence of crenate broomrape disease was estimated for the seven species. The study also provides a morphological description of the polymorphic O. crenata on the samples from Egypt and determines the most useful characteristics for its easier identification in the field.","PeriodicalId":6967,"journal":{"name":"Acta Botanica Croatica","volume":"145 1","pages":""},"PeriodicalIF":1.3,"publicationDate":"2023-11-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139259326","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-11-07DOI: 10.37427/botcro-2024-005
Marija Pandža, Nenad Jasprica
Three individuals of Prangos trifida (Mill.) Herrnst. & Heyn (Apiaceae) were found in Croatia for the first time in 2013. The population has increased in the last ten years and in 2023, 30 individuals were found. Prangos trifida grows on the small islet of Zmorašnji Opuh (Puh) in the Kornati National Park (Dalmatia) within the vegetation of salt-sprayed rocky cliffs. The taxonomic status, ecology and origin of the species are briefly discussed.
{"title":"First record of <i>Prangos trifida</i> (Apiaceae) in Croatia","authors":"Marija Pandža, Nenad Jasprica","doi":"10.37427/botcro-2024-005","DOIUrl":"https://doi.org/10.37427/botcro-2024-005","url":null,"abstract":"Three individuals of Prangos trifida (Mill.) Herrnst. &amp; Heyn (Apiaceae) were found in Croatia for the first time in 2013. The population has increased in the last ten years and in 2023, 30 individuals were found. Prangos trifida grows on the small islet of Zmorašnji Opuh (Puh) in the Kornati National Park (Dalmatia) within the vegetation of salt-sprayed rocky cliffs. The taxonomic status, ecology and origin of the species are briefly discussed.","PeriodicalId":6967,"journal":{"name":"Acta Botanica Croatica","volume":"96 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-11-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135540092","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
A culture-dependent method was used to isolate fungal endophytes from the leaves, stems, and roots of the invasive plant Solanum rostratum Dunal. growing in Xinjiang Province, China. All isolates were identified according to ITS (internal transcribed spacer) region of ribosomal DNA sequences and analyzed by Nucleotide BLAST according to NCBI GenBank and Mycobank database. Altogether 176 endophytic fungal isolates corresponding to 44 OTUs were identified, which were classified into 12 genera, with Penicillium (59.66%) and Aspergillus (23.29%) being the highly dominant genera. Ten endophytic isolates (OTU1, OTU15, OTU16, OTU21, OTU23, OTU25, OTU26, OTU30, OTU37 and OTU44) were identified as potential new species.
采用培养依赖法从入侵植物龙葵(Solanum rostratum Dunal)的叶、茎和根中分离真菌内生菌。生长于中国新疆省。所有分离株根据核糖体DNA ITS (internal transcriptspacer)区序列进行鉴定，并根据NCBI GenBank和Mycobank数据库进行Nucleotide BLAST分析。共鉴定出44个OTUs对应的176株内生真菌，分12属，其中青霉菌(59.66%)和曲霉菌(23.29%)为高度优势属。10株内生菌株(OTU1、OTU15、OTU16、OTU21、OTU23、OTU25、OTU26、OTU30、OTU37和OTU44)被鉴定为潜在新种。
{"title":"Diversity of fungal endophytes isolated from the invasive plant <i>Solanum rostratum</i>","authors":"Nigora Kuchkarova, Caixia Han, Zokir Toshmatov, Hongyang Chen, Hua Shao","doi":"10.37427/botcro-2024-001","DOIUrl":"https://doi.org/10.37427/botcro-2024-001","url":null,"abstract":"A culture-dependent method was used to isolate fungal endophytes from the leaves, stems, and roots of the invasive plant Solanum rostratum Dunal. growing in Xinjiang Province, China. All isolates were identified according to ITS (internal transcribed spacer) region of ribosomal DNA sequences and analyzed by Nucleotide BLAST according to NCBI GenBank and Mycobank database. Altogether 176 endophytic fungal isolates corresponding to 44 OTUs were identified, which were classified into 12 genera, with Penicillium (59.66%) and Aspergillus (23.29%) being the highly dominant genera. Ten endophytic isolates (OTU1, OTU15, OTU16, OTU21, OTU23, OTU25, OTU26, OTU30, OTU37 and OTU44) were identified as potential new species.","PeriodicalId":6967,"journal":{"name":"Acta Botanica Croatica","volume":"1 1","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-11-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135541934","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-10-25DOI: 10.37427/botcro-2024-006
Müjgan Elveren
Due to increased consumption, artificial sweeteners are often present in the environment but their effects on plants are largly unknown. In this research, the effects of four artificial sweeteners on plant stress markers in Triticum aestivum L. were investigated. Wheat seedlings were grown from seeds in soil containing artificial sweeteners (saccharin, sodium cyclamate, sucralose, aspartame) in different concentrations (0, 25, 50, 100 mg kg-1). Plants were irrigated at regular intervals to maintain field capacity moisture and harvested after 15 days of growth. Electrolyte leakage, chlorophyll and carotenoid content, and antioxidant enzyme (superoxide dismutase, peroxidase, catalase) activities were determined in harvested leaves. Comparisons between control samples and test samples were statistically evaluated at a 95% confidence interval to determine significant differences. Overall, significant increases in chlorophyll and carotenoid content, and some antioxidant enzyme activities were observed in wheat plants exposed to artificial sweeteners in the soil. A significant increase in electrolyte leakage was observed with saccharin and aspartame treatment, indicating that these sweeteners can cause membrane damage in wheat. Chlorophyll a nd POX activity were the most sensitive stress parameters in wheat. This study showed the importance of evaluating the potential impact of anthropogenic pollutants that may be present in treated wastewater and consequently affect plants.
{"title":"Effects of artificial sweeteners on antioxidant enzymes and physiological parameters in <i>Triticum aestivum</i> (Poaceae)","authors":"Müjgan Elveren","doi":"10.37427/botcro-2024-006","DOIUrl":"https://doi.org/10.37427/botcro-2024-006","url":null,"abstract":"Due to increased consumption, artificial sweeteners are often present in the environment but their effects on plants are largly unknown. In this research, the effects of four artificial sweeteners on plant stress markers in Triticum aestivum L. were investigated. Wheat seedlings were grown from seeds in soil containing artificial sweeteners (saccharin, sodium cyclamate, sucralose, aspartame) in different concentrations (0, 25, 50, 100 mg kg-1). Plants were irrigated at regular intervals to maintain field capacity moisture and harvested after 15 days of growth. Electrolyte leakage, chlorophyll and carotenoid content, and antioxidant enzyme (superoxide dismutase, peroxidase, catalase) activities were determined in harvested leaves. Comparisons between control samples and test samples were statistically evaluated at a 95% confidence interval to determine significant differences. Overall, significant increases in chlorophyll and carotenoid content, and some antioxidant enzyme activities were observed in wheat plants exposed to artificial sweeteners in the soil. A significant increase in electrolyte leakage was observed with saccharin and aspartame treatment, indicating that these sweeteners can cause membrane damage in wheat. Chlorophyll a nd POX activity were the most sensitive stress parameters in wheat. This study showed the importance of evaluating the potential impact of anthropogenic pollutants that may be present in treated wastewater and consequently affect plants.","PeriodicalId":6967,"journal":{"name":"Acta Botanica Croatica","volume":"41 4","pages":"0"},"PeriodicalIF":0.0,"publicationDate":"2023-10-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"135168745","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-06-02DOI: 10.37427/botcro-2023-014
Aco Teofilovski
Cephalaria pastricensis subsp. pologensis Teofilovski (Dipsacaceae), is published as a new subspecies to science from NW North Macedonia. It is distinguished from C. pastricensis Dörfl. & Hayek subsp. pastricensis by its densely, ± patent-subvillous petioles and rachis of the leaves, and the midrib of the lower surface of the leaf lobes (vs. with sparse, short, ± appressed hairs), densely pubescent lower surface of the leaves (vs. with scattered hairs on the nerves), and densely pubescent to subglabrous upper surface of the leaves (vs. glabrous or with scattered hairs on the nerves). The new subspecies is a Macedonian endemic known from small areas in the Šar Mountains (near Brezno village) and Buković Mt. (near Gorna Đonovica village). Its distributional range is geographically separated from that of C. pastricensis subsp. pastricensis, which is Balkan endemic, distributed in Albania, Montenegro, Bosnia and Herzegovina, Kosovo, and Serbia.
鸭嘴草亚种。Teofilovski波洛根菌（续断科）是北马其顿西北部的一个新亚种。它与C.pastricensis Dörfl不同。&哈耶克亚种。pastriensis的特征是其浓密的、±未被覆盖的亚绒毛叶柄和叶轴，以及叶瓣下表面的中脉（与稀疏的、短的、±贴伏的毛相比），叶下表面密被短柔毛（与神经上有分散的毛相对），叶上表面密被青春期至近无毛（与无毛或神经上有零散的毛）。新亚种是马其顿特有种，分布在Šar山脉（Brezno村附近）和Buković山（Gorna Der onovica村附近）的小地区。其分布范围在地理上与C.pastriensis亚种的分布范围是分开的。pastricensis是巴尔干半岛的特有种，分布于阿尔巴尼亚、黑山、波斯尼亚和黑塞哥维那、科索沃和塞尔维亚。
{"title":"A new subspecies of Cephalaria pastricensis Dörfl. & Hayek (Dipsacaceae) from North Macedonia","authors":"Aco Teofilovski","doi":"10.37427/botcro-2023-014","DOIUrl":"https://doi.org/10.37427/botcro-2023-014","url":null,"abstract":"Cephalaria pastricensis subsp. pologensis Teofilovski (Dipsacaceae), is published as a new subspecies to science from NW North Macedonia. It is distinguished from C. pastricensis Dörfl. & Hayek subsp. pastricensis by its densely, ± patent-subvillous petioles and rachis of the leaves, and the midrib of the lower surface of the leaf lobes (vs. with sparse, short, ± appressed hairs), densely pubescent lower surface of the leaves (vs. with scattered hairs on the nerves), and densely pubescent to subglabrous upper surface of the leaves (vs. glabrous or with scattered hairs on the nerves). The new subspecies is a Macedonian endemic known from small areas in the Šar Mountains (near Brezno village) and Buković Mt. (near Gorna Đonovica village). Its distributional range is geographically separated from that of C. pastricensis subsp. pastricensis, which is Balkan endemic, distributed in Albania, Montenegro, Bosnia and Herzegovina, Kosovo, and Serbia.","PeriodicalId":6967,"journal":{"name":"Acta Botanica Croatica","volume":" ","pages":""},"PeriodicalIF":1.3,"publicationDate":"2023-06-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41434736","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-06-02DOI: 10.37427/botcro-2023-015
Antun Jelinčić, Aleksandra Perčin, Ž. Zgorelec, Dora Papković
In this local-scale synecological study, we investigated the changes in plant community composition throughout secondary succession occurring after cessation of agricultural land use (i.e. grassland abandonment). The successional sequence studied had the following pathway: Avenula pubescens (Huds.) Dumort. haypastures → Brachypodium pinnatum (L.) P.Beauv. successional grassland → Cornus sanguinea L. scrubs → late-successional Populus tremula L. forest → late-successional oak-hornbeam (Quercus-Carpinus) forest. The last forest stage was represented by the association Epimedio-Carpinetum betuli (Horvat 1938) Borhidi 1963. Occurrence of plant species throughout secondary succession was mostly stage-specific; only Fragaria vesca L., Ajuga reptans L., Cornus sanguinea, Prunus spinosa L., and Viola hirta L. showed survival ability throughout almost all stages.
{"title":"Local-scale changes in plant community composition following succession of oak- hornbeam forest after grassland abandonment","authors":"Antun Jelinčić, Aleksandra Perčin, Ž. Zgorelec, Dora Papković","doi":"10.37427/botcro-2023-015","DOIUrl":"https://doi.org/10.37427/botcro-2023-015","url":null,"abstract":"In this local-scale synecological study, we investigated the changes in plant community composition throughout secondary succession occurring after cessation of agricultural land use (i.e. grassland abandonment). The successional sequence studied had the following pathway: Avenula pubescens (Huds.) Dumort. haypastures → Brachypodium pinnatum (L.) P.Beauv. successional grassland → Cornus sanguinea L. scrubs → late-successional Populus tremula L. forest → late-successional oak-hornbeam (Quercus-Carpinus) forest. The last forest stage was represented by the association Epimedio-Carpinetum betuli (Horvat 1938) Borhidi 1963. Occurrence of plant species throughout secondary succession was mostly stage-specific; only Fragaria vesca L., Ajuga reptans L., Cornus sanguinea, Prunus spinosa L., and Viola hirta L. showed survival ability throughout almost all stages.","PeriodicalId":6967,"journal":{"name":"Acta Botanica Croatica","volume":" ","pages":""},"PeriodicalIF":1.3,"publicationDate":"2023-06-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"46618619","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-05-31DOI: 10.37427/botcro-2023-013
G. Sramkó, A. Takács, A. Molnár V., A. Popiela, B. Lukács
The genus Elatine L. consists of ephemeral species of wet-land habitats that live in the temperate regions of both hemispheres. Because of their relatively fast life cycle and small habit, they lead inconspicuous lives, which is probably why they have been relatively little studied in European botany. Although the botanists of the 19 th and early 20 th century discovered all the currently recognised taxa, there were only a few studies that specifically addressed questions on their bi - ology. This lack of detailed knowledge triggered the more recent interest in this genus (especially the European members)
{"title":"Data on species plasticity and stable characters has an overall importance in identification keys: comments on Brullo et al. (2022) article","authors":"G. Sramkó, A. Takács, A. Molnár V., A. Popiela, B. Lukács","doi":"10.37427/botcro-2023-013","DOIUrl":"https://doi.org/10.37427/botcro-2023-013","url":null,"abstract":"The genus Elatine L. consists of ephemeral species of wet-land habitats that live in the temperate regions of both hemispheres. Because of their relatively fast life cycle and small habit, they lead inconspicuous lives, which is probably why they have been relatively little studied in European botany. Although the botanists of the 19 th and early 20 th century discovered all the currently recognised taxa, there were only a few studies that specifically addressed questions on their bi - ology. This lack of detailed knowledge triggered the more recent interest in this genus (especially the European members)","PeriodicalId":6967,"journal":{"name":"Acta Botanica Croatica","volume":" ","pages":""},"PeriodicalIF":1.3,"publicationDate":"2023-05-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"48286731","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-05-31DOI: 10.37427/botcro-2023-012
Nika Pasković, Iris Dupčić Radić
This report presents the first record of Tripos rotundatus (Jørgensen) Gómez in the Adriatic Sea. The species was found in the net sample in the layer of 50 – 100 m depth, on July 2021 at the coastal southern Adriatic Sea. The species T. rotundatus was probably previously mistaken as morphologically similar species T. digitatus (Schütt) Gómez.
{"title":"First record of the dinoflagellate Tripos rotundatus (Jørgensen) Gómez in the Adriatic Sea","authors":"Nika Pasković, Iris Dupčić Radić","doi":"10.37427/botcro-2023-012","DOIUrl":"https://doi.org/10.37427/botcro-2023-012","url":null,"abstract":"This report presents the first record of Tripos rotundatus (Jørgensen) Gómez in the Adriatic Sea. The species was found in the net sample in the layer of 50 – 100 m depth, on July 2021 at the coastal southern Adriatic Sea. The species T. rotundatus was probably previously mistaken as morphologically similar species T. digitatus (Schütt) Gómez.","PeriodicalId":6967,"journal":{"name":"Acta Botanica Croatica","volume":" ","pages":""},"PeriodicalIF":1.3,"publicationDate":"2023-05-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"48646346","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-05-31DOI: 10.37427/botcro-2023-011
Yu. O. Bilonozhko, T. Krupodorova, A. Rabokon, A. Postovoitova, L. Kalafat, Y. Pirko, Y. Blume
To improve the biological control of white mistletoe (European mistletoe) additional detailed information on Botryosphaeria visci infection, its basic nutritional requirements, growth, and in vitro growth characteristics is needed. The objectives of this study were to isolate and identify the fungus B. visci associated with Viscum аlbum from Sorbus aucuparia, to provide information on its in vitro introduction and growth characteristics on different media, and to test the pathogenicity of the isolated fungus. To achieve these goals, the morphology of conidiophores from infected parts of mistletoe was evaluated by microscopy. The isolate from fresh collections of V. album was identified as Sphaeropsis visci anamorph of ascomycete Botryosphaeria visci. The morphology of the vegetative mycelium and growth of B. visci varied depending on the media used. The best medium supporting growth and sporulation was oatmeal. Re-infection of European mistletoe in laboratory conditions showed positive result on liquid media, and in field conditions but only after mechanical damage to the mistletoe leaves. Our results expand the knowledge regarding the optimal cultivation of this fungus. This may facilitate further mycological and pathological studies involving B. visci isolates, and the results have a theoretical basis for the implementation of measures for the prevention and control of mistletoe.
{"title":"In vitro cultivation and biocontrol potential of Botryosphaeria visci against European mistletoe (Viscum album L.)","authors":"Yu. O. Bilonozhko, T. Krupodorova, A. Rabokon, A. Postovoitova, L. Kalafat, Y. Pirko, Y. Blume","doi":"10.37427/botcro-2023-011","DOIUrl":"https://doi.org/10.37427/botcro-2023-011","url":null,"abstract":"To improve the biological control of white mistletoe (European mistletoe) additional detailed information on Botryosphaeria visci infection, its basic nutritional requirements, growth, and in vitro growth characteristics is needed. The objectives of this study were to isolate and identify the fungus B. visci associated with Viscum аlbum from Sorbus aucuparia, to provide information on its in vitro introduction and growth characteristics on different media, and to test the pathogenicity of the isolated fungus. To achieve these goals, the morphology of conidiophores from infected parts of mistletoe was evaluated by microscopy. The isolate from fresh collections of V. album was identified as Sphaeropsis visci anamorph of ascomycete Botryosphaeria visci. The morphology of the vegetative mycelium and growth of B. visci varied depending on the media used. The best medium supporting growth and sporulation was oatmeal. Re-infection of European mistletoe in laboratory conditions showed positive result on liquid media, and in field conditions but only after mechanical damage to the mistletoe leaves. Our results expand the knowledge regarding the optimal cultivation of this fungus. This may facilitate further mycological and pathological studies involving B. visci isolates, and the results have a theoretical basis for the implementation of measures for the prevention and control of mistletoe.","PeriodicalId":6967,"journal":{"name":"Acta Botanica Croatica","volume":"30 4","pages":""},"PeriodicalIF":1.3,"publicationDate":"2023-05-31","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41299989","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-05-19DOI: 10.37427/botcro-2023-008
D. Pavoković, A. Horvatić, I. Tomljanović, B. Balen, M. Krsnik-Rasol
Salt and drought stress are important abiotic factors that negatively affect plant growth and yield. To understand how these stress factors affect metabolism at the cellular level, we analyzed cation concentrations and expression of cellular and extracellular proteins, as well as their functions and types. Cells of the industrially important halophyte sugar beet were exposed to 300 mM NaCl and 600 mM mannitol as stressors in modified Gamborg B5 liquid medium (PG0). Severe stress altered the intracellular concentrations of the most measured cations. The cellular proteome revealed that both stressors provoked significant differential regulation of 110 cellular proteins. About 80% of the identified proteins were classified in metabolism, energy, or cell rescue, defense and virulence categories. We identified several novel proteins that respond to stress, including a member of the bZIP family of transcription factors, a member of the glycine-rich RNA-binding proteins, and the K+ channel beta subunit. Among extracellular proteins we found previously unreported stress-responsive proteins, a beta-xylosidase and an isoform of chitinase. The obtained results indicate that salt and drought stress disturbed the concentrations of cellular cations and the affected expression of cellular and extracellular proteins in sugar beet cells.
{"title":"Sugar beet cells cellular and extracellular events taking place in response to drought and salinity","authors":"D. Pavoković, A. Horvatić, I. Tomljanović, B. Balen, M. Krsnik-Rasol","doi":"10.37427/botcro-2023-008","DOIUrl":"https://doi.org/10.37427/botcro-2023-008","url":null,"abstract":"Salt and drought stress are important abiotic factors that negatively affect plant growth and yield. To understand how these stress factors affect metabolism at the cellular level, we analyzed cation concentrations and expression of cellular and extracellular proteins, as well as their functions and types. Cells of the industrially important halophyte sugar beet were exposed to 300 mM NaCl and 600 mM mannitol as stressors in modified Gamborg B5 liquid medium (PG0). Severe stress altered the intracellular concentrations of the most measured cations. The cellular proteome revealed that both stressors provoked significant differential regulation of 110 cellular proteins. About 80% of the identified proteins were classified in metabolism, energy, or cell rescue, defense and virulence categories. We identified several novel proteins that respond to stress, including a member of the bZIP family of transcription factors, a member of the glycine-rich RNA-binding proteins, and the K+ channel beta subunit. Among extracellular proteins we found previously unreported stress-responsive proteins, a beta-xylosidase and an isoform of chitinase. The obtained results indicate that salt and drought stress disturbed the concentrations of cellular cations and the affected expression of cellular and extracellular proteins in sugar beet cells.","PeriodicalId":6967,"journal":{"name":"Acta Botanica Croatica","volume":"18 6","pages":""},"PeriodicalIF":1.3,"publicationDate":"2023-05-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41290715","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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