Weipixiao ameliorates gastric precancerous lesions in a rat's model by regulating GSK3β and C-myc

IF 2 4区医学 Q2 INTEGRATIVE & COMPLEMENTARY MEDICINE Journal of Traditional Chinese Medicine Pub Date : 2018-10-01 DOI:10.1016/S0254-6272(18)30909-9

Zeng Jinhao , Pan Huafeng , Guo Jing , Gong Daoyin , Cai Tiantian , Chen Xiaodong , Zhang Yi , You Fengming , Chen Longhui , Zhao Ziming , Liang Chao

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引用次数: 3

Abstract

OBJECTIVE

To investigate the mechanism underlying the action of Weipixiao (WPX) in a rat's model with ameliorating gastric precancerous lesions (GPL).

METHODS

HPLC analysis was performed to identify the chemical constituents of WPX preparation. Sprague- Dawley rats were randomly assigned into control group, model group, vitacoenzyme group, high-dose WPX group (H-WPX), medium-dose WPX group (M-WPX) and low-dose WPX group (L-WPX). After modeling, the treated rats were administrated WPX or vitacoenzyme intragastrically for consecutive 10 weeks. Gene and protein expressions of GSK3β, C-myc, Cylin E were evaluated by quantitative real-time reverse transcription-polymerase chain reaction (RT-qPCR) and immunohistochemistry, respectively.

RESULTS

WPX could efficiently attenuate the pathological alterations of “non-progressive GPL” in rats. As expected, mRNA and protein levels of C-myc and Cylin E were up-regulated in model rats, while GSK3β expression down-regulated (P < 0.01). WPX treatment, especially at low dose, could significantly down-regulate the mRNA as well as protein levels of C-myc, and could lead to remarkable up-regulation of mRNA and protein levels of GSK3β in GPL rats (P < 0.05). However, no significant changes were observed in WPX-treated rats.

CONCLUSION

Our findings suggested that WPX-mediated attenuation of GPL pathological alterations might be due to its regulatory effect on the expressions of GSK3β and C-myc, and on the dysregulation of Wnt/GSK3β pathway.

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胃皮消通过调节GSK3β和C-myc改善大鼠胃癌前病变模型

目的探讨胃脾消(WPX)对大鼠胃癌前病变(GPL)的改善作用机制。方法采用高效液相色谱法对WPX制剂进行化学成分鉴定。将Sprague- Dawley大鼠随机分为对照组、模型组、维生素酶组、高剂量WPX组(H-WPX)、中剂量WPX组(M-WPX)和低剂量WPX组(L-WPX)。造模后，给药大鼠灌胃WPX或维酵素，连续10周。采用实时定量逆转录聚合酶链反应(RT-qPCR)和免疫组织化学分别检测GSK3β、C-myc、Cylin E基因和蛋白的表达。结果swpx能有效减轻大鼠“非进行性GPL”的病理改变。正如预期的那样，模型大鼠C-myc和Cylin E的mRNA和蛋白水平上调，而GSK3β的表达下调(P <0.01)。WPX处理，特别是低剂量处理，可以显著下调GPL大鼠C-myc mRNA和蛋白水平，并导致GSK3β mRNA和蛋白水平显著上调(P <0.05)。然而，在wpx治疗的大鼠中没有观察到明显的变化。结论wpx介导的GPL病理改变的减弱可能与其对GSK3β和C-myc的表达以及对Wnt/GSK3β通路失调的调节作用有关。

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来源期刊

Journal of Traditional Chinese Medicine INTEGRATIVE & COMPLEMENTARY MEDICINE-

CiteScore

2.40

自引率

3.80%

发文量

32269

审稿时长

2 months

期刊介绍： Journal of Traditional Chinese Medicine（JTCM） is devoted to clinical and theortical research on the use of acupuncture and Oriental medicine. The main columns include Clinical Observations, Basic Investigations, Reviews, Questions and Answers, an Expert''s Forum, and Discussions of Clinical Cases. Its key topics include acupuncture and electro-acupuncture, herbal medicine, homeopathy, masseotherapy, mind-body therapies, palliative care, and other CAM therapies.