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Comparative study of the effects of Danhong injection with different doses on ischemic stroke: A substudy of hospital-based Danhong injection registry 不同剂量丹红注射液对缺血性脑卒中疗效的比较研究:以医院为基础的丹红注射液登记亚研究
IF 2.6 4区 医学 Q2 INTEGRATIVE & COMPLEMENTARY MEDICINE Pub Date : 2018-12-15 DOI: 10.1016/S0254-6272(18)30992-0
Du Yawei , Wang Xiaoxia , Zhong Liqun , Wu Shengxian

OBJECTIVE

To compare the effects of Danhong injection (DHI) with different doses on ischemic stroke.

METHODS

Chinese hospital registered DHI study is a perspective observational study, in which a total of 1292 patients with ischemic stroke were treated with DHI. Two different doses of DHI were used in the present study. The patients were matched by acute phase of ischemic stroke (within 2 weeks after onset), partial anterior circulation infraction, sex, age, and National Institute of Health Stroke Scale (NIHSS) score. Finally 148 patients were grouped into the 20 or 40 mL DHI group with 74 in each. The primary endpoint was the improvement of NIHSS score at 14-day after treatment.

RESULTS

Of the 148 patients with ischemic stroke, the 74 in the 40 mL DHI group had clinically important improvements in the NIHSS score. The baseline and 14-day NIHSS score for the 40 mL DHI group were 6.6 ± 4.3 and 2.4 ± 2.5 respectively, versus 6.3 ± 5.7 and 3.6 ± 4.2, for the 20 mL DHI group (change from baseline in the 40 mL DHI group vs change from baseline in the 20 mL DHI group, −1.49 points; P = 0.029). Improvements were maintained after the baseline characteristics and other drugs were controlled (P = 0.012). No adverse events were observed.

CONCLUSION

Effects of DHI on partial anterior circulation infarction in patients with acute phase of ischemic stroke is dose-dependent; however, further studies with larger sample sizes are needed to validate our findings (Chinese Clinical Trial Register, Number: ChiCTR-ONC-13003230).

目的比较不同剂量丹红注射液对缺血性脑卒中的治疗作用。方法中国医院注册DHI研究是一项前瞻性观察性研究,共1292例缺血性脑卒中患者接受DHI治疗。本研究中使用了两种不同剂量的DHI。根据缺血性卒中急性期(发病后2周内)、部分前循环梗塞、性别、年龄和美国国立卫生研究院卒中量表(NIHSS)评分进行匹配。148例患者被分为20和40 mL DHI组,每组74例。主要终点是治疗后14天NIHSS评分的改善。结果148例缺血性脑卒中患者中,40 mL DHI组74例患者NIHSS评分有显著改善。40 mL DHI组的基线和14天NIHSS评分分别为6.6±4.3和2.4±2.5,而20 mL DHI组为6.3±5.7和3.6±4.2 (40 mL DHI组与20 mL DHI组的基线变化,−1.49分;P = 0.029)。在基线特征和其他药物得到控制后,改善得以维持(P = 0.012)。未观察到不良事件。结论DHI对缺血性脑卒中急性期部分前循环梗死的影响呈剂量依赖性;然而,需要进一步的更大样本量的研究来验证我们的发现(中国临床试验注册,编号:ChiCTR-ONC-13003230)。
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引用次数: 5
Acupoint combinations used for treatment of Alzheimer's disease: A data mining analysis 穴位组合治疗阿尔茨海默病:数据挖掘分析
IF 2.6 4区 医学 Q2 INTEGRATIVE & COMPLEMENTARY MEDICINE Pub Date : 2018-12-15 DOI: 10.1016/S0254-6272(18)30995-6
Yu Chaochao , Wang Li , Kong Lihong , Shen Feng , Ma Chaoyang , Du Yanjun , Zhou Hua

OBJECTIVE

To identify the acupoint combinations used in the treatment of Alzheimer's disease (AD).

METHODS

The clinical literature regarding acupuncture and moxibustion for AD was searched and collected from databases including Chinese Biomedical Medicine, China National Knowledge Infrastructure, Wanfang Database and PubMed. The database of acupuncture and moxibustion prescriptions for AD was established by using Excel software so as to conduct the descriptive analysis, association analysis on the data.

RESULTS

Baihui (GV 20), Sishencong (EX-HN 1), Shenmen (HT 7), Zusanli (ST 36), Neiguan (PC 6), Fengchi (GB 20), Taixi (KI 3), Dazhui (GV 14), Shenshu (BL 23), Sanyinjiao (SP 6), Shenting (GV 24), Fenglong (ST 40), Xuanzhong (GB 39), Shuigou (GV 26) and Taichong (LR 3) were of higher frequency in the treatment of AD with acupnucture and moxibustion. Most acupoints were selected from the Governor Vessel. The commonly used acupoints were located on the head, face, neck and lower limbs. The combination of the local acupoints with the distal ones was predominated. The crossing points among the specific points presented the advantage in the treatment. The association analysis indicated that the correlation among Fengchi (GB 20)-Baihui (GV 20) was the strongest, followed by combinations of Dazhui (GV 14)-Baihui (GV 20), Shenshu (BL 23)- Baihui (GV 20) and Neiguan (PC 6)- Baihui (GV 20) and indicated the common rules of the clinical acupoint selection and combination for AD.

CONCLUSION

Our findings provide a reference for acupoints selection and combination for AD in clinical acupuncture practice.

目的探讨治疗阿尔茨海默病(AD)的腧穴组合。方法从中国生物医学数据库、中国国家知识基础设施数据库、万方数据库和PubMed数据库中检索和收集针灸治疗AD的临床文献。运用Excel软件建立AD针灸方剂数据库,对数据进行描述性分析、关联分析。结果百会(GV 20)、四神聪(EX-HN 1)、神门(HT 7)、足三里(ST 36)、内关(PC 6)、风池(gb20)、太溪(KI 3)、大椎(GV 14)、肾疏(b23)、三阴交(SP 6)、神汀(GV 24)、凤龙(ST 40)、宣中(gb39)、水沟(GV 26)、太冲(lr3)是针灸治疗AD的高发部位。大多数穴位选自督脉。常用穴位位于头部、面部、颈部和下肢。以局部穴位与远端穴位相结合为主。特异点之间的交点在处理中表现出优势。关联分析显示风池(gb20)-百会(gv20)的相关性最强,大椎(gv14)-百会(gv20)、肾疏(b23)-百会(GV 20)、内关(pc6)-百会(GV 20)的相关性其次,提示了AD临床中西医结合的一般规律。结论本研究结果可为临床针灸治疗AD的取穴与配合提供参考。
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引用次数: 19
Factors associated with seeking western or Chinese medical treatment for fertility among women with breast cancer in Taiwan 台湾乳腺癌妇女寻求西医或中医治疗生育的相关因素
IF 2.6 4区 医学 Q2 INTEGRATIVE & COMPLEMENTARY MEDICINE Pub Date : 2018-12-15 DOI: 10.1016/S0254-6272(18)30990-7
Ping-Ho Chen , Sheng-Miauh Huang , Jerry Cheng-Yen Lai , Chen-Jei Tai , Li-Yin Chien , Yun-Hsiang Lee

OBJECTIVE

To examine and describe the use of Western and Chinese Medicine for pregnancy preparation among women with breast cancer after cancer-related treatment in Taiwan.

METHODS

Women of reproductive age (20-49 years) women who were treated for breast cancer from January 2011 through June 2015 in a Taiwanese city participated in the study. Of 306 eligible study participants, interviews were completed in 197 (64%). Participants were asked about sociode-mographic variables, disease and treatment characteristics, their desire for fertility, and their use of Western or Chinese Medicine.

RESULTS

The proportions of women who planned to use Western or Chinese Medicine to help achieve pregnancy were 17.3% and 14.7%, respectively. The result of binary logistic regression showed that current employment and lack of children were factors predictive of the use of Western Medicine. Younger age and the use of Chinese Medicine before treatment were predictors of using Chinese Medicine. While the desire for fertility preservation was related to the use of Western Medicine, the desire for pregnancy was related to the use of Chinese Medicine.

CONCLUSION

Various characteristics guided the women's decisions to use Western or Chinese Medicine. Health care providers should learn patients' preferences for fertility preservation and provide appropriate advice, referring them to safe providers of their desired method of fertility treatment.

目的了解台湾地区乳腺癌患者接受癌症相关治疗后中西医结合备孕的情况。方法2011年1月至2015年6月在台湾某城市接受乳腺癌治疗的育龄妇女(20-49岁)参与了这项研究。在306名符合条件的研究参与者中,197人(64%)完成了访谈。参与者被问及社会人口统计学变量、疾病和治疗特征、他们对生育的渴望以及他们对西医或中医的使用。结果计划使用西药或中药助孕的比例分别为17.3%和14.7%。二元logistic回归分析结果显示,当前就业和无子女是西药使用的预测因素。年龄较小和治疗前使用中药是使用中药的预测因素。保留生育能力的愿望与使用西医有关,而怀孕的愿望与使用中医有关。结论不同的特点指导了妇女选择西药或中药。卫生保健提供者应了解患者对保留生育能力的偏好,并提供适当的建议,将其转介给提供其所需生育治疗方法的安全提供者。
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引用次数: 0
Hormone-like activities of Kuntai capsule in the uteri of ovariectomized rats and immature rabbits 坤泰胶囊在去卵巢大鼠和未成熟家兔子宫内的激素样活性
IF 2.6 4区 医学 Q2 INTEGRATIVE & COMPLEMENTARY MEDICINE Pub Date : 2018-12-15 DOI: 10.1016/S0254-6272(18)30984-1
Zhong Ruihua, Xie Shuwu, Li Guoting, Yang Wenjie, Guo Xiangjie, Li Zhao, Zhou Jieyun, Ma Aying, Zhu Yan

OBJECTIVE

To investigate the hormone-like activities of Kuntai capsule (KTC) in the uteri of ovariectomized rats and immature rabbits.

METHODS

Following bilateral ovariectomy, rats were randomly divided into six groups including sham group, control group, estradiol valerate group, KTC 0.24, 0.6, and 1.5 g/kg groups. The rats were treated with 0.5% CMC-Na, estradiol valerate and KTC (0.24, 0.6, and 1.5 g/kg), respectively for 28 consecutive days. Then the estrous cycle, uterine changes and pathological changes were examined. Serum levels of estradiol (E2), and progesterone (P4) were measured by enzyme-linked immunosorbent assay (ELISA). Protein levels of estradiol receptor (ER), progesterone receptor (PR), vascular endothelial growth factor (VEGF), proliferating cell nuclear antigen (PCNA) and nuclear-associated antigen-67 (Ki-67) in uterine tissues were detected by western blot. Immature rabbits were estrogen-primed prior to intragastric administration with KTC for 6 d consecutively. Then, the uteri underwent hematoxylin-eosin staining to observe endometrial transformation.

RESULTS

Compared with the control group, 0.6 and 1.5 g/kg KTC markedly decreased the uterine organ coefficient and endometrial thickness (P < 0.05). The serum level of P4 was increased in the KTC 0.6 g/kg group (P < 0.05). There were no significant variations in the serum level of E2 in the KTC groups compared with the control group. ERβ, but not ERα, was markedly upregulated after KTC administration (P < 0.05). Furthermore, 1.5 g/kg KTC significantly decreased the protein level of PRA (P < 0.05) and 0.6 g/kg KTC increased the protein level of PRB in the uteri (P < 0.05). VEGF was highly expressed after treatment with 0.24 and 0.6 g/kg KTC, and Ki-67 was markedly reduced in ovariectomized rats treated with 1.5 g/kg KTC. No difference was found in the expression of PCNA. KTC 0.24 and 0.6 g/kg promoted endometrial transformation in immature rabbit uteri.

CONCLUSION

KTC does not demonstrate obvious estrogen-like effect on uteri after ovariectomy, but it does exhibit weak progestogen-like effect, by which mechanism of action is yet to be further investigated.

目的观察坤泰胶囊(KTC)在去卵巢大鼠和未成熟家兔子宫内的激素样活性。方法双侧卵巢切除术后,将大鼠随机分为假手术组、对照组、戊酸雌二醇组、KTC 0.24、0.6、1.5 g/kg组。大鼠分别给予0.5% CMC-Na、戊酸雌二醇和KTC(0.24、0.6和1.5 g/kg),连续28 d。观察大鼠的发情周期、子宫变化及病理变化。采用酶联免疫吸附试验(ELISA)检测血清雌二醇(E2)、孕酮(P4)水平。western blot法检测子宫组织中雌二醇受体(ER)、孕酮受体(PR)、血管内皮生长因子(VEGF)、增殖细胞核抗原(PCNA)和核相关抗原-67 (Ki-67)的蛋白水平。未成熟家兔在连续灌胃KTC 6 d前进行雌激素刺激。然后行苏木精-伊红染色观察子宫内膜转化。结果与对照组比较,0.6、1.5 g/kg KTC可显著降低子宫器官系数和子宫内膜厚度(P <0.05)。0.6 g/kg KTC组血清P4水平升高(P <0.05)。与对照组相比,KTC组血清E2水平无显著变化。给药后,ERβ显著上调,而ERα未显著上调(P <0.05)。此外,1.5 g/kg KTC显著降低了PRA蛋白水平(P <0.05), 0.6 g/kg KTC可提高子宫PRB蛋白水平(P <0.05)。0.24和0.6 g/kg KTC处理后VEGF高表达,1.5 g/kg KTC处理的去卵巢大鼠Ki-67明显降低。PCNA表达差异无统计学意义。KTC 0.24和0.6 g/kg促进未成熟兔子宫内膜转化。结论ktc对卵巢切除术后子宫无明显的类雌激素作用,但有较弱的类黄体酮作用,其作用机制有待进一步研究。
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引用次数: 2
Tanshinone II A improves distribution and anti-tumor efficacy of pegylated liposomal doxorubicin via normalizing the structure and function of tumor vasculature in hepa1-6 hepatoma mice model 丹参酮II A通过调节hepa1-6肝癌小鼠模型肿瘤血管的结构和功能,改善聚乙二醇脂质体阿霉素的分布和抗肿瘤作用
IF 2.6 4区 医学 Q2 INTEGRATIVE & COMPLEMENTARY MEDICINE Pub Date : 2018-12-15 DOI: 10.1016/S0254-6272(18)30980-4
Zhang Ying , Tie Minghui , Bi Feng , Wang Ke

OBJECTIVE

To investigate whether the Tan II A could improve the distribution and anti-tumor efficacy of Pegylated Liposomal Doxorubicin (PLD) via normalizing the structure and function of vasculature in Hepa1-6 hepatoma mice model.

METHODS

Hepa1-6 hepatoma-bearing mice were treated with Tan II A for 14 d. Distribution and anti-tumor efficacy of PLD, and the structure and function of the tumor vasculature were evaluated using various techniques.

RESULTS

Tan II A significantly reduced the micro-vessel density (MVD). After Tan II A treatment, the tumor vascular walls were better structured, as the increased coverage of the pericytes and the promoted contact of the basement membrane and endothelial cell. Functional tests showed that tumor hypoxia was improved and the exudation amount of Evans blue in the parenchyma of the tumor decreased. In addition, mice treated with Tan II A had greater PLD penetration distance intratumorally. Furthermore, combined therapy of Tan II A and PLD significantly inhibited tumor growth.

CONCLUSION

This study suggests that Tan II A helps normalizing the tumor vasculature and has therapeutic potential in increasing the distribution of chemotherapy drug in the tumor.

目的探讨Tan II A是否能通过调节Hepa1-6肝癌小鼠的血管结构和功能,改善聚乙二醇化脂质体阿霉素(PLD)的分布及抗肿瘤作用。方法用Tan II A治疗shepa1 -6肝癌小鼠14 d,采用多种方法观察PLD的分布、抗肿瘤效果以及肿瘤血管的结构和功能。结果stanⅱA显著降低微血管密度(MVD)。经TanⅱA治疗后,肿瘤血管壁结构改善,周细胞覆盖率增加,基底膜与内皮细胞接触增强。功能检查显示肿瘤缺氧改善,肿瘤实质内埃文斯蓝渗出量减少。此外,用Tan II A治疗的小鼠瘤内PLD穿透距离更大。此外,TanⅱA与PLD联合治疗可显著抑制肿瘤生长。结论TanⅱA有助于肿瘤血管的正常化,在增加化疗药物在肿瘤中的分布方面具有治疗潜力。
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引用次数: 6
Traditional Chinese Medicine syndrome elements of male infertility revealed by latent tree model analysis 潜在树模型分析揭示男性不育症的中医证候要素
IF 2.6 4区 医学 Q2 INTEGRATIVE & COMPLEMENTARY MEDICINE Pub Date : 2018-12-15 DOI: 10.1016/S0254-6272(18)30993-2
Zhang Zhijie , Liu Shaoming , Zhang Yueyang , Yang Jingzhe , Kong Tao , Wang Chengli , Ning Peng , Chen Xiaochao , Li Xuesong , Jia Yusen , Chen Xiaojun

OBJECTIVE

To explore the features of Traditional Chinese Medicine (TCM) syndromes in male infertility using computer-based analyses.

METHODS

Latent class analysis was used to analyze the TCM syndrome data from 813 patients with male infertility and establish a latent tree model.

RESULTS

A latent tree model with a Bayesian information criterion score of – 11 263 was created. This model revealed that the characteristics of basic TCM syndromes in patients with male infertility were kidney Yang deficiency, kidney Qi deficiency, spleen Yang deficiency, liver Qi stagnation, Qi stagnation and blood stasis, and dump-heat; moreover, most patients with male infertility had complex syndromes (spleen-kidney Yang deficiency and liver Qi stagnation) rather than simple single syndromes.

CONCLUSION

The hidden tree model analysis revealed the objective and quantitative complex relationships between the TCM symptoms of male infertility, and obtained the quantification and objective evidence of TCM syndromes in male infertility.

目的利用计算机分析方法探讨男性不育症中医证候特征。方法采用潜类分析方法,对813例男性不育症中医证候资料进行分析,建立潜树模型。结果建立了贝叶斯信息准则得分为- 11 263的sa潜树模型。该模型揭示了男性不育症患者的基本中医证候特征为肾阳虚、肾气虚、脾阳虚、肝郁、气滞血瘀、郁热;而且,男性不育症患者大多有脾肾阳虚、肝郁等复合证型,而不是单纯的单一证型。结论隐含树模型分析揭示了男性不育症中医证候之间客观与定量的复杂关系,获得了男性不育症中医证候的定量化和客观证据。
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引用次数: 8
Inhibitive effect of loureirin B plus capsaicin on tetrodotoxin-resistant sodium channel 罗瑞林B联合辣椒素对河豚毒素抗性钠通道的抑制作用
IF 2.6 4区 医学 Q2 INTEGRATIVE & COMPLEMENTARY MEDICINE Pub Date : 2018-12-15 DOI: 10.1016/S0254-6272(18)30983-X
Chen Su , Wan Ying , Liu Xiangming , Pan Xinxin

OBJECTIVE

To investigate whether the effect of loureirin B plus capsaicin on tetrodotoxin-resistant (TTX-R) sodium channel.

METHODS

By using whole-cell patch-clamp recordings, in acutely isolated dorsal root ganglion (DRG) neurons, the combined effects of loureirin B and capsaicin on TTX-R sodium channel were observed. Based on the data, the interaction between loureirin B and capsaicin in their modulation on TTX-R sodium channel was assessed.

RESULTS

Loureirin B could not induce transient inward TRPV1 current. Capsazepine, a transient receptor potential vanilloid l (TRPV1) antagonist, could not attenuate the block of 0.64 mmol/L loureirin B on TTX-R sodium channel. There was no significant difference (P > 0.05) between IC50 of loureirin B (0.37 mmol/L) on TTX-R sodium channel in capsaicin-sensitive DRG neurons and that (0.38 mmol/L) in capsaicin-insensitive DRG neurons. However, there was a significant difference (P < 0.05) between the IC50 of capsaicin (0.28 μmol/L) on TTX-R sodium channel in capsaicin-sensitive DRG neurons and that (52.24 μmol/L) in capsaicin-insensitive DRG neurons. Four combinations composed of various concentrations of loureirin B and capsaicin could all inhibit TTX-R sodium currents but have different interactions between loureirin B and capsaicin.

CONCLUSION

Loureirin B plus capsaicin could produce double blockage on TRPV1 and modulation on TTX-R sodium channel. The action of loureirin B on TTX-R sodium channel was independent of TRPV1 but similar with that of capsaicin on TTX-R sodium channel in capsaicin-insensitive DRG neurons.

目的探讨紫红素B联合辣椒素对河豚毒素耐药钠通道的影响。方法采用全细胞膜片钳法,观察洛瑞素B和辣椒素对急性离体背根神经节(DRG)神经元TTX-R钠通道的联合作用。在此基础上,研究了辣素B和辣椒素对TTX-R钠通道的相互作用。结果sloureirin B不能诱导瞬时内向TRPV1电流。瞬时受体电位香草素1 (TRPV1)拮抗剂Capsazepine不能减弱0.64 mmol/ l loureirin B对TTX-R钠通道的阻滞。差异无统计学意义(P >在辣椒素敏感的DRG神经元TTX-R钠通道上,loureirin B的IC50为0.37 mmol/L,与辣椒素不敏感的DRG神经元的IC50为0.38 mmol/L。然而,有显著差异(P <辣椒素对敏感DRG神经元TTX-R钠通道的IC50为0.28 μmol/L,对不敏感DRG神经元TTX-R钠通道的IC50为52.24 μmol/L。不同浓度的灯叶苷B与辣椒素组成的4种组合均能抑制TTX-R钠电流,但灯叶苷B与辣椒素的相互作用不同。结论洛瑞素B联合辣椒素可双重阻断TRPV1并调节TTX-R钠通道。辣素B对TTX-R钠通道的作用不依赖于TRPV1,但与辣椒素对辣椒素不敏感DRG神经元TTX-R钠通道的作用相似。
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引用次数: 5
Possible mechanism underlying analgesic effect of Tuina in rats may involve piezo mechanosensitive channels within dorsal root ganglia axon 推拿对大鼠镇痛作用的机制可能与背根神经节轴突内的压电机械敏感通道有关
IF 2.6 4区 医学 Q2 INTEGRATIVE & COMPLEMENTARY MEDICINE Pub Date : 2018-12-15 DOI: 10.1016/S0254-6272(18)30982-8
Song Pengfei , Lin Zhigang , Zhu Qingguang , Jiang Shichao , Zhang Hao , Cheng Yanbin , Sun Wuquan , Wu Zhiwei , Zhou Xin , Lü Zhizhen , Fang Min

OBJECTIVE

To demonstrate the analgesic effect of Tuina mainly from mechanically sensitive ion channels in peripheral myelinated nerve fibers.

METHODS

A total of 40 healthy and pathogen-free adult male Sprague-Dawley rats were used in the study [weight: (220.0 ± 1.4) g, Shanghai Slac Laboratory Animal Co., Ltd., Shanghai, China; license No. Shanghai ICP 05033115]. The rats were housed in cages with free access to water and food in a temperature-controlled room [(22 ± 1) °C] and 12-h/12-h light-dark cycle. Thirty-two rats were randomly divided into five groups: naive, sham, chronic compression of dorsal root ganglion (CCD), Tuina (7 d) and Tuina (21 d). CCD rat model was established via unilateral DRG compression by “L” liked steel bar. Chinese Tuina treatment was accepted once per day. Behavior monitoring of paw withdrawal threshold (PWT) and paw withdrawal latency (PWL) were tested. The expression of Piezo1 and Piezo2 in myelinated nerve fiber were analyzed by immunohistochemistry and Western-blotting.

RESULTS

There was a high expression of Piezo2 and a low expression of Piezo1 in the naive and CCD groups. In contrast, the expression of Piezo2 was down regulated and Piezo1 was increased after a period of Tuina. There was significant difference (P ≤ 0.05) between the groups.

CONCLUSION

Our findings suggest that Tuina therapy can increase the expression of Piezo2 and decrease the expression of Piezo1 in the test rats. The different changes in the expressions of Piezo1 and Piezo2 may play an important role in alleviating CCD-induced allodynia and hyperalgesia.

目的探讨推拿主要通过外周髓鞘神经纤维的机械敏感离子通道进行镇痛作用。方法选用健康、无病原体的成年雄性Sprague-Dawley大鼠40只[体重:(220.0±1.4)g,上海斯莱克实验动物有限公司,上海,中国;许可证没有。上海ICP 05033115]。将大鼠置于温度控制[(22±1)°C]的自由饮水和食物的笼子中,光照周期为12 h/12 h。32只大鼠随机分为5组:单纯组、假组、慢性背根神经节压迫组、推拿组(7 d)和推拿组(21 d),采用“L”形钢条单侧压迫DRG建立CCD大鼠模型。采用中医推拿治疗,每日1次。实验采用足爪戒断阈值(PWT)和足爪戒断潜伏期(PWL)进行行为监测。采用免疫组织化学和Western-blotting分析了Piezo1和Piezo2在髓鞘神经纤维中的表达。结果在未感染组和CCD组中,Piezo2高表达,Piezo1低表达。相反,按摩一段时间后,Piezo2表达下调,而Piezo1表达升高。两组间差异有统计学意义(P≤0.05)。结论推拿治疗可提高大鼠的Piezo2表达,降低Piezo1表达。Piezo1和Piezo2表达的不同变化可能在缓解ccd诱导的异常性痛和痛觉过敏中起重要作用。
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引用次数: 11
Blood flow differences in cun-kou (radial) artery and anterior tibial artery: normal people vs patients with chronic gastritis 村口(桡)动脉和胫前动脉血流的差异:正常人与慢性胃炎患者
IF 2.6 4区 医学 Q2 INTEGRATIVE & COMPLEMENTARY MEDICINE Pub Date : 2018-12-15 DOI: 10.1016/S0254-6272(18)30991-9
Wang Chao , Wei Yun , Zhang Lin , Song Jia , Liu Ni , Fu Yanling

OBJECTIVE

To explore the blood flow differences in cun-kou (radial) artery and anterior tibial artery between normal people and patients with chronic gastritis

METHODS

Using doppler ultrasonography, blood flow charts [peak systolic velocity (VP), maximum blood flow velocity in diastole (VD), mean blood flow velocity (VM), pulse index (PI), resistance index (RI), blood vessel diameter (D), vessel volume (SV), circulation blood flow periodic time (ET)] measured by at cun-kou (radial) artery and anterior tibial artery in normal group (n = 30) and chronic gastritis group (n = 30) in department of ultrasound, Beijing Anzhen hospital, capital medical university.

RESULTS

In the doppler flow charts of the normal group, there were statistically significant differences in VD, VM, D, RI, SV, ET between cun-kou artery and anterior tibial artery (P < 0.05), and there were more statistically significant differences in PI between cun-kou artery and anterior tibial artery (P < 0.01). In the comparison of doppler flow charts in the chronic gastritis group, there were statistically significant differences in VD, VM, D, RI, SV, ET between cun-kou artery and anterior tibial artery (P < 0.05), and there were more statistically significant differences in PI between cun-kou artery and anterior tibial artery (P < 0.01). In the comparison of doppler flow charts between normal group and chronic gastritis group, there were statistically significant differences in SV, ET between normal group and chronic gastritis group (P < 0.05), and there were more statistically significant differences in PI between normal group and chronic gastritis group (P <0.01).

CONCLUSION

Based on the differences in doppler flow charts between the normal group and the chronic gastritis group, the doppler flow charts of cun-kou artery and anterior tibial artery were not only proved to be significantly different, but also provided quantitative objective indexes for the study of cun-kou artery and anterior tibial artery doppler flow charts of normal people and chronic gastritis patients. This study also proves that the doppler flow chart of the anterior tibial pulse is of great significance for the diagnosis of chronic gastritis.

目的探讨慢性胃炎患者与正常人桡动脉、胫前动脉血流的差异。方法采用多普勒超声检查,绘制血流图[收缩期峰值血流速度(VP)、舒张期最大血流速度(VD)、平均血流速度(VM)、脉搏指数(PI)、阻力指数(RI)、血管直径(D)、血管容积(SV)、血管收缩速度(VP)、血管舒张期最大血流速度(VD)]。首都医科大学附属北京安贞医院超声科用正常组(n = 30)和慢性胃炎组(n = 30)的桡动脉、胫前动脉循环血流量周期时间(ET)测定正常组(n = 30)和慢性胃炎组(n = 30)。结果正常组多普勒血流图显示,村口动脉与胫前动脉VD、VM、D、RI、SV、ET差异均有统计学意义(P <P < 0.05),且村口动脉与胫前动脉PI差异有统计学意义(P <0.01)。慢性胃炎组多普勒血流图比较,村口动脉与胫前动脉VD、VM、D、RI、SV、ET差异有统计学意义(P <P < 0.05),且村口动脉与胫前动脉PI差异有统计学意义(P <0.01)。正常组与慢性胃炎组多普勒血流图比较,正常组与慢性胃炎组SV、ET比较,差异有统计学意义(P <慢性胃炎组与正常组PI比较,差异有统计学意义(P <0.01)。结论通过正常组和慢性胃炎组多普勒血流图的差异,不仅证明了村口动脉和胫前动脉多普勒血流图存在显著性差异,而且为正常人和慢性胃炎患者的村口动脉和胫前动脉多普勒血流图的研究提供了定量的客观指标。本研究也证实了胫骨前脉多普勒血流图对慢性胃炎的诊断具有重要意义。
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引用次数: 0
Involvement of heme oxygenase-1 induction in anti-vascular inflammation effects of Xanthoceras sorbifolia in human umbilical vein endothelial cells 血红素加氧酶-1诱导参与文冠果对人脐静脉内皮细胞的抗血管炎症作用
IF 2.6 4区 医学 Q2 INTEGRATIVE & COMPLEMENTARY MEDICINE Pub Date : 2018-12-15 DOI: 10.1016/S0254-6272(18)30979-8
Jung Joo Yoon , Byung Hyuk Han , Eun Sik Choi , Seung Namgung , Da Hye Jeong , Song Nan Jin , Yun Jung Lee , Dae Gill Kang , Ho Sub Lee

OBJECTIVE

To define the effects of Xanthoceras sorbifolia (EXS) on vascular inflammation and the mechanisms in endothelial cells.

METHODS

Vascular protective effects of an ethanol extract of seeds from EXS (1-50 μg/mL) against tumor necrosis factor-α (TNF-α)-induced vascular inflammation were examined in human umbilical vein endothelial cells (HUVECs).

RESULTS

EXS significantly decreased TNF-α-induced expression of cell adhesion molecules, such as intracellular adhesion molecule-1, vascular cell adhesion molecule-1, and endothelial cell selectin, in a dose-dependent manner. Pre-treatment with EXS significantly inhibited translocation and transcriptional activity of nuclear factor-κB (NF-κB) increased by TNF-α. EXS also significantly inhibited formation of intracellular reactive oxygen species (ROS). Moreover, the vascular protective effects of EXS were linked to up-regulation of heme oxygenase-1 (HO-1) and nuclear factor E2-related factor-2 (Nrf-2) expression. EXS-induced HO-1 expression was significantly decreased in SnPP (HO-1 inhibitor)- and HO-1 siRNA-treated cells, whereas an increase was found in cobalt protoporphyrin IX (CoPP) (HO-1 inducer)-treated cells. In addition, pretreatment with EXS increased HO-1 and Nrf-2 expression under TNF-α stimulation with or without N-acetyl-L-cysteine. Furthermore, the inhibitory effects of EXS on TNF-α-induced vascular inflammation were partially reversed in SnPP- and of HO-1 siRNA-treated cells but increased by CoPP.

CONCLUSION

These results suggest that EXS may have important implications for prevention of vascular complications associated with vascular inflammation by inhibition of the NF-κB/ROS pathway and activation of the Nrf-2/HO-1 pathway.

目的探讨文冠果(EXS)对血管炎症的影响及其作用机制。方法观察EXS种子乙醇提取物(1 ~ 50 μg/mL)对人脐静脉内皮细胞(HUVECs)肿瘤坏死因子-α (TNF-α)诱导的血管炎症的保护作用。结果sexs显著降低TNF-α-诱导的细胞黏附分子(细胞内黏附分子-1、血管细胞黏附分子-1、内皮细胞选择素)的表达,并呈剂量依赖性。EXS预处理可显著抑制核因子-κB (NF-κB)的易位,NF-κB的转录活性因TNF-α而升高。EXS还能显著抑制细胞内活性氧(ROS)的形成。此外,EXS的血管保护作用与血红素氧合酶-1 (HO-1)和核因子e2相关因子-2 (Nrf-2)表达上调有关。exs诱导的HO-1表达在SnPP (HO-1抑制剂)和HO-1 sirna处理的细胞中显著降低,而在钴原卟啉IX (CoPP) (HO-1诱导剂)处理的细胞中升高。此外,在TNF-α刺激或不刺激n -乙酰半胱氨酸的情况下,EXS预处理可增加HO-1和Nrf-2的表达。此外,EXS对TNF-α-诱导的血管炎症的抑制作用在SnPP-和HO-1 sirna处理的细胞中部分逆转,但在CoPP处理的细胞中增强。结论EXS可能通过抑制NF-κB/ROS通路和激活Nrf-2/HO-1通路,对预防血管炎症相关并发症具有重要意义。
{"title":"Involvement of heme oxygenase-1 induction in anti-vascular inflammation effects of Xanthoceras sorbifolia in human umbilical vein endothelial cells","authors":"Jung Joo Yoon ,&nbsp;Byung Hyuk Han ,&nbsp;Eun Sik Choi ,&nbsp;Seung Namgung ,&nbsp;Da Hye Jeong ,&nbsp;Song Nan Jin ,&nbsp;Yun Jung Lee ,&nbsp;Dae Gill Kang ,&nbsp;Ho Sub Lee","doi":"10.1016/S0254-6272(18)30979-8","DOIUrl":"10.1016/S0254-6272(18)30979-8","url":null,"abstract":"<div><h3>OBJECTIVE</h3><p>To define the effects of Xanthoceras sorbifolia (EXS) on vascular inflammation and the mechanisms in endothelial cells.</p></div><div><h3>METHODS</h3><p>Vascular protective effects of an ethanol extract of seeds from EXS (1-50 μg/mL) against tumor necrosis factor-α (TNF-α)-induced vascular inflammation were examined in human umbilical vein endothelial cells (HUVECs).</p></div><div><h3>RESULTS</h3><p>EXS significantly decreased TNF-α-induced expression of cell adhesion molecules, such as intracellular adhesion molecule-1, vascular cell adhesion molecule-1, and endothelial cell selectin, in a dose-dependent manner. Pre-treatment with EXS significantly inhibited translocation and transcriptional activity of nuclear factor-κB (NF-κB) increased by TNF-α. EXS also significantly inhibited formation of intracellular reactive oxygen species (ROS). Moreover, the vascular protective effects of EXS were linked to up-regulation of heme oxygenase-1 (HO-1) and nuclear factor E2-related factor-2 (Nrf-2) expression. EXS-induced HO-1 expression was significantly decreased in SnPP (HO-1 inhibitor)- and HO-1 siRNA-treated cells, whereas an increase was found in cobalt protoporphyrin IX (CoPP) (HO-1 inducer)-treated cells. In addition, pretreatment with EXS increased HO-1 and Nrf-2 expression under TNF-α stimulation with or without N-acetyl-L-cysteine. Furthermore, the inhibitory effects of EXS on TNF-α-induced vascular inflammation were partially reversed in SnPP- and of HO-1 siRNA-treated cells but increased by CoPP.</p></div><div><h3>CONCLUSION</h3><p>These results suggest that EXS may have important implications for prevention of vascular complications associated with vascular inflammation by inhibition of the NF-κB/ROS pathway and activation of the Nrf-2/HO-1 pathway.</p></div>","PeriodicalId":17513,"journal":{"name":"Journal of Traditional Chinese Medicine","volume":"38 6","pages":"Pages 803-814"},"PeriodicalIF":2.6,"publicationDate":"2018-12-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/S0254-6272(18)30979-8","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"42460618","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 5
期刊
Journal of Traditional Chinese Medicine
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