Pub Date : 2018-12-15DOI: 10.1016/S0254-6272(18)30992-0
Du Yawei , Wang Xiaoxia , Zhong Liqun , Wu Shengxian
OBJECTIVE
To compare the effects of Danhong injection (DHI) with different doses on ischemic stroke.
METHODS
Chinese hospital registered DHI study is a perspective observational study, in which a total of 1292 patients with ischemic stroke were treated with DHI. Two different doses of DHI were used in the present study. The patients were matched by acute phase of ischemic stroke (within 2 weeks after onset), partial anterior circulation infraction, sex, age, and National Institute of Health Stroke Scale (NIHSS) score. Finally 148 patients were grouped into the 20 or 40 mL DHI group with 74 in each. The primary endpoint was the improvement of NIHSS score at 14-day after treatment.
RESULTS
Of the 148 patients with ischemic stroke, the 74 in the 40 mL DHI group had clinically important improvements in the NIHSS score. The baseline and 14-day NIHSS score for the 40 mL DHI group were 6.6 ± 4.3 and 2.4 ± 2.5 respectively, versus 6.3 ± 5.7 and 3.6 ± 4.2, for the 20 mL DHI group (change from baseline in the 40 mL DHI group vs change from baseline in the 20 mL DHI group, −1.49 points; P = 0.029). Improvements were maintained after the baseline characteristics and other drugs were controlled (P = 0.012). No adverse events were observed.
CONCLUSION
Effects of DHI on partial anterior circulation infarction in patients with acute phase of ischemic stroke is dose-dependent; however, further studies with larger sample sizes are needed to validate our findings (Chinese Clinical Trial Register, Number: ChiCTR-ONC-13003230).
目的比较不同剂量丹红注射液对缺血性脑卒中的治疗作用。方法中国医院注册DHI研究是一项前瞻性观察性研究，共1292例缺血性脑卒中患者接受DHI治疗。本研究中使用了两种不同剂量的DHI。根据缺血性卒中急性期(发病后2周内)、部分前循环梗塞、性别、年龄和美国国立卫生研究院卒中量表(NIHSS)评分进行匹配。148例患者被分为20和40 mL DHI组，每组74例。主要终点是治疗后14天NIHSS评分的改善。结果148例缺血性脑卒中患者中，40 mL DHI组74例患者NIHSS评分有显著改善。40 mL DHI组的基线和14天NIHSS评分分别为6.6±4.3和2.4±2.5，而20 mL DHI组为6.3±5.7和3.6±4.2 (40 mL DHI组与20 mL DHI组的基线变化，−1.49分;P = 0.029)。在基线特征和其他药物得到控制后，改善得以维持(P = 0.012)。未观察到不良事件。结论DHI对缺血性脑卒中急性期部分前循环梗死的影响呈剂量依赖性;然而，需要进一步的更大样本量的研究来验证我们的发现(中国临床试验注册，编号:ChiCTR-ONC-13003230)。
{"title":"Comparative study of the effects of Danhong injection with different doses on ischemic stroke: A substudy of hospital-based Danhong injection registry","authors":"Du Yawei , Wang Xiaoxia , Zhong Liqun , Wu Shengxian","doi":"10.1016/S0254-6272(18)30992-0","DOIUrl":"10.1016/S0254-6272(18)30992-0","url":null,"abstract":"<div><h3>OBJECTIVE</h3><p>To compare the effects of Danhong injection (DHI) with different doses on ischemic stroke.</p></div><div><h3>METHODS</h3><p>Chinese hospital registered DHI study is a perspective observational study, in which a total of 1292 patients with ischemic stroke were treated with DHI. Two different doses of DHI were used in the present study. The patients were matched by acute phase of ischemic stroke (within 2 weeks after onset), partial anterior circulation infraction, sex, age, and National Institute of Health Stroke Scale (NIHSS) score. Finally 148 patients were grouped into the 20 or 40 mL DHI group with 74 in each. The primary endpoint was the improvement of NIHSS score at 14-day after treatment.</p></div><div><h3>RESULTS</h3><p>Of the 148 patients with ischemic stroke, the 74 in the 40 mL DHI group had clinically important improvements in the NIHSS score. The baseline and 14-day NIHSS score for the 40 mL DHI group were 6.6 ± 4.3 and 2.4 ± 2.5 respectively, versus 6.3 ± 5.7 and 3.6 ± 4.2, for the 20 mL DHI group (change from baseline in the 40 mL DHI group <em>vs</em> change from baseline in the 20 mL DHI group, −1.49 points; <em>P</em> = 0.029). Improvements were maintained after the baseline characteristics and other drugs were controlled (<em>P</em> = 0.012). No adverse events were observed.</p></div><div><h3>CONCLUSION</h3><p>Effects of DHI on partial anterior circulation infarction in patients with acute phase of ischemic stroke is dose-dependent; however, further studies with larger sample sizes are needed to validate our findings (Chinese Clinical Trial Register, Number: ChiCTR-ONC-13003230).</p></div>","PeriodicalId":17513,"journal":{"name":"Journal of Traditional Chinese Medicine","volume":null,"pages":null},"PeriodicalIF":2.6,"publicationDate":"2018-12-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/S0254-6272(18)30992-0","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"49034355","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2018-12-15DOI: 10.1016/S0254-6272(18)30995-6
Yu Chaochao , Wang Li , Kong Lihong , Shen Feng , Ma Chaoyang , Du Yanjun , Zhou Hua
OBJECTIVE
To identify the acupoint combinations used in the treatment of Alzheimer's disease (AD).
METHODS
The clinical literature regarding acupuncture and moxibustion for AD was searched and collected from databases including Chinese Biomedical Medicine, China National Knowledge Infrastructure, Wanfang Database and PubMed. The database of acupuncture and moxibustion prescriptions for AD was established by using Excel software so as to conduct the descriptive analysis, association analysis on the data.
RESULTS
Baihui (GV 20), Sishencong (EX-HN 1), Shenmen (HT 7), Zusanli (ST 36), Neiguan (PC 6), Fengchi (GB 20), Taixi (KI 3), Dazhui (GV 14), Shenshu (BL 23), Sanyinjiao (SP 6), Shenting (GV 24), Fenglong (ST 40), Xuanzhong (GB 39), Shuigou (GV 26) and Taichong (LR 3) were of higher frequency in the treatment of AD with acupnucture and moxibustion. Most acupoints were selected from the Governor Vessel. The commonly used acupoints were located on the head, face, neck and lower limbs. The combination of the local acupoints with the distal ones was predominated. The crossing points among the specific points presented the advantage in the treatment. The association analysis indicated that the correlation among Fengchi (GB 20)-Baihui (GV 20) was the strongest, followed by combinations of Dazhui (GV 14)-Baihui (GV 20), Shenshu (BL 23)- Baihui (GV 20) and Neiguan (PC 6)- Baihui (GV 20) and indicated the common rules of the clinical acupoint selection and combination for AD.
CONCLUSION
Our findings provide a reference for acupoints selection and combination for AD in clinical acupuncture practice.
{"title":"Acupoint combinations used for treatment of Alzheimer's disease: A data mining analysis","authors":"Yu Chaochao , Wang Li , Kong Lihong , Shen Feng , Ma Chaoyang , Du Yanjun , Zhou Hua","doi":"10.1016/S0254-6272(18)30995-6","DOIUrl":"10.1016/S0254-6272(18)30995-6","url":null,"abstract":"<div><h3>OBJECTIVE</h3><p>To identify the acupoint combinations used in the treatment of Alzheimer's disease (AD).</p></div><div><h3>METHODS</h3><p>The clinical literature regarding acupuncture and moxibustion for AD was searched and collected from databases including Chinese Biomedical Medicine, China National Knowledge Infrastructure, Wanfang Database and PubMed. The database of acupuncture and moxibustion prescriptions for AD was established by using Excel software so as to conduct the descriptive analysis, association analysis on the data.</p></div><div><h3>RESULTS</h3><p>Baihui (GV 20), Sishencong (EX-HN 1), Shenmen (HT 7), Zusanli (ST 36), Neiguan (PC 6), Fengchi (GB 20), Taixi (KI 3), Dazhui (GV 14), Shenshu (BL 23), Sanyinjiao (SP 6), Shenting (GV 24), Fenglong (ST 40), Xuanzhong (GB 39), Shuigou (GV 26) and Taichong (LR 3) were of higher frequency in the treatment of AD with acupnucture and moxibustion. Most acupoints were selected from the Governor Vessel. The commonly used acupoints were located on the head, face, neck and lower limbs. The combination of the local acupoints with the distal ones was predominated. The crossing points among the specific points presented the advantage in the treatment. The association analysis indicated that the correlation among Fengchi (GB 20)-Baihui (GV 20) was the strongest, followed by combinations of Dazhui (GV 14)-Baihui (GV 20), Shenshu (BL 23)- Baihui (GV 20) and Neiguan (PC 6)- Baihui (GV 20) and indicated the common rules of the clinical acupoint selection and combination for AD.</p></div><div><h3>CONCLUSION</h3><p>Our findings provide a reference for acupoints selection and combination for AD in clinical acupuncture practice.</p></div>","PeriodicalId":17513,"journal":{"name":"Journal of Traditional Chinese Medicine","volume":null,"pages":null},"PeriodicalIF":2.6,"publicationDate":"2018-12-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/S0254-6272(18)30995-6","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"46536158","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2018-12-15DOI: 10.1016/S0254-6272(18)30990-7
Ping-Ho Chen , Sheng-Miauh Huang , Jerry Cheng-Yen Lai , Chen-Jei Tai , Li-Yin Chien , Yun-Hsiang Lee
OBJECTIVE
To examine and describe the use of Western and Chinese Medicine for pregnancy preparation among women with breast cancer after cancer-related treatment in Taiwan.
METHODS
Women of reproductive age (20-49 years) women who were treated for breast cancer from January 2011 through June 2015 in a Taiwanese city participated in the study. Of 306 eligible study participants, interviews were completed in 197 (64%). Participants were asked about sociode-mographic variables, disease and treatment characteristics, their desire for fertility, and their use of Western or Chinese Medicine.
RESULTS
The proportions of women who planned to use Western or Chinese Medicine to help achieve pregnancy were 17.3% and 14.7%, respectively. The result of binary logistic regression showed that current employment and lack of children were factors predictive of the use of Western Medicine. Younger age and the use of Chinese Medicine before treatment were predictors of using Chinese Medicine. While the desire for fertility preservation was related to the use of Western Medicine, the desire for pregnancy was related to the use of Chinese Medicine.
CONCLUSION
Various characteristics guided the women's decisions to use Western or Chinese Medicine. Health care providers should learn patients' preferences for fertility preservation and provide appropriate advice, referring them to safe providers of their desired method of fertility treatment.
{"title":"Factors associated with seeking western or Chinese medical treatment for fertility among women with breast cancer in Taiwan","authors":"Ping-Ho Chen , Sheng-Miauh Huang , Jerry Cheng-Yen Lai , Chen-Jei Tai , Li-Yin Chien , Yun-Hsiang Lee","doi":"10.1016/S0254-6272(18)30990-7","DOIUrl":"10.1016/S0254-6272(18)30990-7","url":null,"abstract":"<div><h3>OBJECTIVE</h3><p>To examine and describe the use of Western and Chinese Medicine for pregnancy preparation among women with breast cancer after cancer-related treatment in Taiwan.</p></div><div><h3>METHODS</h3><p>Women of reproductive age (20-49 years) women who were treated for breast cancer from January 2011 through June 2015 in a Taiwanese city participated in the study. Of 306 eligible study participants, interviews were completed in 197 (64%). Participants were asked about sociode-mographic variables, disease and treatment characteristics, their desire for fertility, and their use of Western or Chinese Medicine.</p></div><div><h3>RESULTS</h3><p>The proportions of women who planned to use Western or Chinese Medicine to help achieve pregnancy were 17.3% and 14.7%, respectively. The result of binary logistic regression showed that current employment and lack of children were factors predictive of the use of Western Medicine. Younger age and the use of Chinese Medicine before treatment were predictors of using Chinese Medicine. While the desire for fertility preservation was related to the use of Western Medicine, the desire for pregnancy was related to the use of Chinese Medicine.</p></div><div><h3>CONCLUSION</h3><p>Various characteristics guided the women's decisions to use Western or Chinese Medicine. Health care providers should learn patients' preferences for fertility preservation and provide appropriate advice, referring them to safe providers of their desired method of fertility treatment.</p></div>","PeriodicalId":17513,"journal":{"name":"Journal of Traditional Chinese Medicine","volume":null,"pages":null},"PeriodicalIF":2.6,"publicationDate":"2018-12-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/S0254-6272(18)30990-7","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"48899950","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2018-12-15DOI: 10.1016/S0254-6272(18)30984-1
Zhong Ruihua, Xie Shuwu, Li Guoting, Yang Wenjie, Guo Xiangjie, Li Zhao, Zhou Jieyun, Ma Aying, Zhu Yan
OBJECTIVE
To investigate the hormone-like activities of Kuntai capsule (KTC) in the uteri of ovariectomized rats and immature rabbits.
METHODS
Following bilateral ovariectomy, rats were randomly divided into six groups including sham group, control group, estradiol valerate group, KTC 0.24, 0.6, and 1.5 g/kg groups. The rats were treated with 0.5% CMC-Na, estradiol valerate and KTC (0.24, 0.6, and 1.5 g/kg), respectively for 28 consecutive days. Then the estrous cycle, uterine changes and pathological changes were examined. Serum levels of estradiol (E2), and progesterone (P4) were measured by enzyme-linked immunosorbent assay (ELISA). Protein levels of estradiol receptor (ER), progesterone receptor (PR), vascular endothelial growth factor (VEGF), proliferating cell nuclear antigen (PCNA) and nuclear-associated antigen-67 (Ki-67) in uterine tissues were detected by western blot. Immature rabbits were estrogen-primed prior to intragastric administration with KTC for 6 d consecutively. Then, the uteri underwent hematoxylin-eosin staining to observe endometrial transformation.
RESULTS
Compared with the control group, 0.6 and 1.5 g/kg KTC markedly decreased the uterine organ coefficient and endometrial thickness (P < 0.05). The serum level of P4 was increased in the KTC 0.6 g/kg group (P < 0.05). There were no significant variations in the serum level of E2 in the KTC groups compared with the control group. ERβ, but not ERα, was markedly upregulated after KTC administration (P < 0.05). Furthermore, 1.5 g/kg KTC significantly decreased the protein level of PRA (P < 0.05) and 0.6 g/kg KTC increased the protein level of PRB in the uteri (P < 0.05). VEGF was highly expressed after treatment with 0.24 and 0.6 g/kg KTC, and Ki-67 was markedly reduced in ovariectomized rats treated with 1.5 g/kg KTC. No difference was found in the expression of PCNA. KTC 0.24 and 0.6 g/kg promoted endometrial transformation in immature rabbit uteri.
CONCLUSION
KTC does not demonstrate obvious estrogen-like effect on uteri after ovariectomy, but it does exhibit weak progestogen-like effect, by which mechanism of action is yet to be further investigated.
{"title":"Hormone-like activities of Kuntai capsule in the uteri of ovariectomized rats and immature rabbits","authors":"Zhong Ruihua, Xie Shuwu, Li Guoting, Yang Wenjie, Guo Xiangjie, Li Zhao, Zhou Jieyun, Ma Aying, Zhu Yan","doi":"10.1016/S0254-6272(18)30984-1","DOIUrl":"10.1016/S0254-6272(18)30984-1","url":null,"abstract":"<div><h3>OBJECTIVE</h3><p>To investigate the hormone-like activities of Kuntai capsule (KTC) in the uteri of ovariectomized rats and immature rabbits.</p></div><div><h3>METHODS</h3><p>Following bilateral ovariectomy, rats were randomly divided into six groups including sham group, control group, estradiol valerate group, KTC 0.24, 0.6, and 1.5 g/kg groups. The rats were treated with 0.5% CMC-Na, estradiol valerate and KTC (0.24, 0.6, and 1.5 g/kg), respectively for 28 consecutive days. Then the estrous cycle, uterine changes and pathological changes were examined. Serum levels of estradiol (E2), and progesterone (P4) were measured by enzyme-linked immunosorbent assay (ELISA). Protein levels of estradiol receptor (ER), progesterone receptor (PR), vascular endothelial growth factor (VEGF), proliferating cell nuclear antigen (PCNA) and nuclear-associated antigen-67 (Ki-67) in uterine tissues were detected by western blot. Immature rabbits were estrogen-primed prior to intragastric administration with KTC for 6 d consecutively. Then, the uteri underwent hematoxylin-eosin staining to observe endometrial transformation.</p></div><div><h3>RESULTS</h3><p>Compared with the control group, 0.6 and 1.5 g/kg KTC markedly decreased the uterine organ coefficient and endometrial thickness (<em>P</em> < 0.05). The serum level of P4 was increased in the KTC 0.6 g/kg group (<em>P</em> < 0.05). There were no significant variations in the serum level of E2 in the KTC groups compared with the control group. ERβ, but not ERα, was markedly upregulated after KTC administration (<em>P</em> < 0.05). Furthermore, 1.5 g/kg KTC significantly decreased the protein level of PRA (<em>P</em> < 0.05) and 0.6 g/kg KTC increased the protein level of PRB in the uteri (<em>P</em> < 0.05). VEGF was highly expressed after treatment with 0.24 and 0.6 g/kg KTC, and Ki-67 was markedly reduced in ovariectomized rats treated with 1.5 g/kg KTC. No difference was found in the expression of PCNA. KTC 0.24 and 0.6 g/kg promoted endometrial transformation in immature rabbit uteri.</p></div><div><h3>CONCLUSION</h3><p>KTC does not demonstrate obvious estrogen-like effect on uteri after ovariectomy, but it does exhibit weak progestogen-like effect, by which mechanism of action is yet to be further investigated.</p></div>","PeriodicalId":17513,"journal":{"name":"Journal of Traditional Chinese Medicine","volume":null,"pages":null},"PeriodicalIF":2.6,"publicationDate":"2018-12-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/S0254-6272(18)30984-1","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"48108788","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2018-12-15DOI: 10.1016/S0254-6272(18)30980-4
Zhang Ying , Tie Minghui , Bi Feng , Wang Ke
OBJECTIVE
To investigate whether the Tan II A could improve the distribution and anti-tumor efficacy of Pegylated Liposomal Doxorubicin (PLD) via normalizing the structure and function of vasculature in Hepa1-6 hepatoma mice model.
METHODS
Hepa1-6 hepatoma-bearing mice were treated with Tan II A for 14 d. Distribution and anti-tumor efficacy of PLD, and the structure and function of the tumor vasculature were evaluated using various techniques.
RESULTS
Tan II A significantly reduced the micro-vessel density (MVD). After Tan II A treatment, the tumor vascular walls were better structured, as the increased coverage of the pericytes and the promoted contact of the basement membrane and endothelial cell. Functional tests showed that tumor hypoxia was improved and the exudation amount of Evans blue in the parenchyma of the tumor decreased. In addition, mice treated with Tan II A had greater PLD penetration distance intratumorally. Furthermore, combined therapy of Tan II A and PLD significantly inhibited tumor growth.
CONCLUSION
This study suggests that Tan II A helps normalizing the tumor vasculature and has therapeutic potential in increasing the distribution of chemotherapy drug in the tumor.
目的探讨Tan II A是否能通过调节Hepa1-6肝癌小鼠的血管结构和功能，改善聚乙二醇化脂质体阿霉素(PLD)的分布及抗肿瘤作用。方法用Tan II A治疗shepa1 -6肝癌小鼠14 d，采用多种方法观察PLD的分布、抗肿瘤效果以及肿瘤血管的结构和功能。结果stanⅱA显著降低微血管密度(MVD)。经TanⅱA治疗后，肿瘤血管壁结构改善，周细胞覆盖率增加，基底膜与内皮细胞接触增强。功能检查显示肿瘤缺氧改善，肿瘤实质内埃文斯蓝渗出量减少。此外，用Tan II A治疗的小鼠瘤内PLD穿透距离更大。此外，TanⅱA与PLD联合治疗可显著抑制肿瘤生长。结论TanⅱA有助于肿瘤血管的正常化，在增加化疗药物在肿瘤中的分布方面具有治疗潜力。
{"title":"Tanshinone II A improves distribution and anti-tumor efficacy of pegylated liposomal doxorubicin via normalizing the structure and function of tumor vasculature in hepa1-6 hepatoma mice model","authors":"Zhang Ying , Tie Minghui , Bi Feng , Wang Ke","doi":"10.1016/S0254-6272(18)30980-4","DOIUrl":"10.1016/S0254-6272(18)30980-4","url":null,"abstract":"<div><h3>OBJECTIVE</h3><p>To investigate whether the Tan II A could improve the distribution and anti-tumor efficacy of Pegylated Liposomal Doxorubicin (PLD) <em>via</em> normalizing the structure and function of vasculature in Hepa1-6 hepatoma mice model.</p></div><div><h3>METHODS</h3><p>Hepa1-6 hepatoma-bearing mice were treated with Tan II A for 14 d. Distribution and anti-tumor efficacy of PLD, and the structure and function of the tumor vasculature were evaluated using various techniques.</p></div><div><h3>RESULTS</h3><p>Tan II A significantly reduced the micro-vessel density (MVD). After Tan II A treatment, the tumor vascular walls were better structured, as the increased coverage of the pericytes and the promoted contact of the basement membrane and endothelial cell. Functional tests showed that tumor hypoxia was improved and the exudation amount of Evans blue in the parenchyma of the tumor decreased. In addition, mice treated with Tan II A had greater PLD penetration distance intratumorally. Furthermore, combined therapy of Tan II A and PLD significantly inhibited tumor growth.</p></div><div><h3>CONCLUSION</h3><p>This study suggests that Tan II A helps normalizing the tumor vasculature and has therapeutic potential in increasing the distribution of chemotherapy drug in the tumor.</p></div>","PeriodicalId":17513,"journal":{"name":"Journal of Traditional Chinese Medicine","volume":null,"pages":null},"PeriodicalIF":2.6,"publicationDate":"2018-12-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/S0254-6272(18)30980-4","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"48714493","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2018-12-15DOI: 10.1016/S0254-6272(18)30993-2
Zhang Zhijie , Liu Shaoming , Zhang Yueyang , Yang Jingzhe , Kong Tao , Wang Chengli , Ning Peng , Chen Xiaochao , Li Xuesong , Jia Yusen , Chen Xiaojun
OBJECTIVE
To explore the features of Traditional Chinese Medicine (TCM) syndromes in male infertility using computer-based analyses.
METHODS
Latent class analysis was used to analyze the TCM syndrome data from 813 patients with male infertility and establish a latent tree model.
RESULTS
A latent tree model with a Bayesian information criterion score of – 11 263 was created. This model revealed that the characteristics of basic TCM syndromes in patients with male infertility were kidney Yang deficiency, kidney Qi deficiency, spleen Yang deficiency, liver Qi stagnation, Qi stagnation and blood stasis, and dump-heat; moreover, most patients with male infertility had complex syndromes (spleen-kidney Yang deficiency and liver Qi stagnation) rather than simple single syndromes.
CONCLUSION
The hidden tree model analysis revealed the objective and quantitative complex relationships between the TCM symptoms of male infertility, and obtained the quantification and objective evidence of TCM syndromes in male infertility.
{"title":"Traditional Chinese Medicine syndrome elements of male infertility revealed by latent tree model analysis","authors":"Zhang Zhijie , Liu Shaoming , Zhang Yueyang , Yang Jingzhe , Kong Tao , Wang Chengli , Ning Peng , Chen Xiaochao , Li Xuesong , Jia Yusen , Chen Xiaojun","doi":"10.1016/S0254-6272(18)30993-2","DOIUrl":"10.1016/S0254-6272(18)30993-2","url":null,"abstract":"<div><h3>OBJECTIVE</h3><p>To explore the features of Traditional Chinese Medicine (TCM) syndromes in male infertility using computer-based analyses.</p></div><div><h3>METHODS</h3><p>Latent class analysis was used to analyze the TCM syndrome data from 813 patients with male infertility and establish a latent tree model.</p></div><div><h3>RESULTS</h3><p>A latent tree model with a Bayesian information criterion score of – 11 263 was created. This model revealed that the characteristics of basic TCM syndromes in patients with male infertility were kidney <em>Yang</em> deficiency, kidney <em>Qi</em> deficiency, spleen <em>Yang</em> deficiency, liver <em>Qi</em> stagnation, <em>Qi</em> stagnation and blood stasis, and dump-heat; moreover, most patients with male infertility had complex syndromes (spleen-kidney <em>Yang</em> deficiency and liver <em>Qi</em> stagnation) rather than simple single syndromes.</p></div><div><h3>CONCLUSION</h3><p>The hidden tree model analysis revealed the objective and quantitative complex relationships between the TCM symptoms of male infertility, and obtained the quantification and objective evidence of TCM syndromes in male infertility.</p></div>","PeriodicalId":17513,"journal":{"name":"Journal of Traditional Chinese Medicine","volume":null,"pages":null},"PeriodicalIF":2.6,"publicationDate":"2018-12-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/S0254-6272(18)30993-2","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"47198979","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2018-12-15DOI: 10.1016/S0254-6272(18)30983-X
Chen Su , Wan Ying , Liu Xiangming , Pan Xinxin
OBJECTIVE
To investigate whether the effect of loureirin B plus capsaicin on tetrodotoxin-resistant (TTX-R) sodium channel.
METHODS
By using whole-cell patch-clamp recordings, in acutely isolated dorsal root ganglion (DRG) neurons, the combined effects of loureirin B and capsaicin on TTX-R sodium channel were observed. Based on the data, the interaction between loureirin B and capsaicin in their modulation on TTX-R sodium channel was assessed.
RESULTS
Loureirin B could not induce transient inward TRPV1 current. Capsazepine, a transient receptor potential vanilloid l (TRPV1) antagonist, could not attenuate the block of 0.64 mmol/L loureirin B on TTX-R sodium channel. There was no significant difference (P > 0.05) between IC50 of loureirin B (0.37 mmol/L) on TTX-R sodium channel in capsaicin-sensitive DRG neurons and that (0.38 mmol/L) in capsaicin-insensitive DRG neurons. However, there was a significant difference (P < 0.05) between the IC50 of capsaicin (0.28 μmol/L) on TTX-R sodium channel in capsaicin-sensitive DRG neurons and that (52.24 μmol/L) in capsaicin-insensitive DRG neurons. Four combinations composed of various concentrations of loureirin B and capsaicin could all inhibit TTX-R sodium currents but have different interactions between loureirin B and capsaicin.
CONCLUSION
Loureirin B plus capsaicin could produce double blockage on TRPV1 and modulation on TTX-R sodium channel. The action of loureirin B on TTX-R sodium channel was independent of TRPV1 but similar with that of capsaicin on TTX-R sodium channel in capsaicin-insensitive DRG neurons.
{"title":"Inhibitive effect of loureirin B plus capsaicin on tetrodotoxin-resistant sodium channel","authors":"Chen Su , Wan Ying , Liu Xiangming , Pan Xinxin","doi":"10.1016/S0254-6272(18)30983-X","DOIUrl":"10.1016/S0254-6272(18)30983-X","url":null,"abstract":"<div><h3>OBJECTIVE</h3><p>To investigate whether the effect of loureirin B plus capsaicin on tetrodotoxin-resistant (TTX-R) sodium channel.</p></div><div><h3>METHODS</h3><p>By using whole-cell patch-clamp recordings, in acutely isolated dorsal root ganglion (DRG) neurons, the combined effects of loureirin B and capsaicin on TTX-R sodium channel were observed. Based on the data, the interaction between loureirin B and capsaicin in their modulation on TTX-R sodium channel was assessed.</p></div><div><h3>RESULTS</h3><p>Loureirin B could not induce transient inward TRPV1 current. Capsazepine, a transient receptor potential vanilloid l (TRPV1) antagonist, could not attenuate the block of 0.64 mmol/L loureirin B on TTX-R sodium channel. There was no significant difference (<em>P</em> > 0.05) between IC<sub>50</sub> of loureirin B (0.37 mmol/L) on TTX-R sodium channel in capsaicin-sensitive DRG neurons and that (0.38 mmol/L) in capsaicin-insensitive DRG neurons. However, there was a significant difference (<em>P</em> < 0.05) between the IC<sub>50</sub> of capsaicin (0.28 μmol/L) on TTX-R sodium channel in capsaicin-sensitive DRG neurons and that (52.24 μmol/L) in capsaicin-insensitive DRG neurons. Four combinations composed of various concentrations of loureirin B and capsaicin could all inhibit TTX-R sodium currents but have different interactions between loureirin B and capsaicin.</p></div><div><h3>CONCLUSION</h3><p>Loureirin B plus capsaicin could produce double blockage on TRPV1 and modulation on TTX-R sodium channel. The action of loureirin B on TTX-R sodium channel was independent of TRPV1 but similar with that of capsaicin on TTX-R sodium channel in capsaicin-insensitive DRG neurons.</p></div>","PeriodicalId":17513,"journal":{"name":"Journal of Traditional Chinese Medicine","volume":null,"pages":null},"PeriodicalIF":2.6,"publicationDate":"2018-12-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/S0254-6272(18)30983-X","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"47259914","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2018-12-15DOI: 10.1016/S0254-6272(18)30979-8
Jung Joo Yoon , Byung Hyuk Han , Eun Sik Choi , Seung Namgung , Da Hye Jeong , Song Nan Jin , Yun Jung Lee , Dae Gill Kang , Ho Sub Lee
OBJECTIVE
To define the effects of Xanthoceras sorbifolia (EXS) on vascular inflammation and the mechanisms in endothelial cells.
METHODS
Vascular protective effects of an ethanol extract of seeds from EXS (1-50 μg/mL) against tumor necrosis factor-α (TNF-α)-induced vascular inflammation were examined in human umbilical vein endothelial cells (HUVECs).
RESULTS
EXS significantly decreased TNF-α-induced expression of cell adhesion molecules, such as intracellular adhesion molecule-1, vascular cell adhesion molecule-1, and endothelial cell selectin, in a dose-dependent manner. Pre-treatment with EXS significantly inhibited translocation and transcriptional activity of nuclear factor-κB (NF-κB) increased by TNF-α. EXS also significantly inhibited formation of intracellular reactive oxygen species (ROS). Moreover, the vascular protective effects of EXS were linked to up-regulation of heme oxygenase-1 (HO-1) and nuclear factor E2-related factor-2 (Nrf-2) expression. EXS-induced HO-1 expression was significantly decreased in SnPP (HO-1 inhibitor)- and HO-1 siRNA-treated cells, whereas an increase was found in cobalt protoporphyrin IX (CoPP) (HO-1 inducer)-treated cells. In addition, pretreatment with EXS increased HO-1 and Nrf-2 expression under TNF-α stimulation with or without N-acetyl-L-cysteine. Furthermore, the inhibitory effects of EXS on TNF-α-induced vascular inflammation were partially reversed in SnPP- and of HO-1 siRNA-treated cells but increased by CoPP.
CONCLUSION
These results suggest that EXS may have important implications for prevention of vascular complications associated with vascular inflammation by inhibition of the NF-κB/ROS pathway and activation of the Nrf-2/HO-1 pathway.
{"title":"Involvement of heme oxygenase-1 induction in anti-vascular inflammation effects of Xanthoceras sorbifolia in human umbilical vein endothelial cells","authors":"Jung Joo Yoon , Byung Hyuk Han , Eun Sik Choi , Seung Namgung , Da Hye Jeong , Song Nan Jin , Yun Jung Lee , Dae Gill Kang , Ho Sub Lee","doi":"10.1016/S0254-6272(18)30979-8","DOIUrl":"10.1016/S0254-6272(18)30979-8","url":null,"abstract":"<div><h3>OBJECTIVE</h3><p>To define the effects of Xanthoceras sorbifolia (EXS) on vascular inflammation and the mechanisms in endothelial cells.</p></div><div><h3>METHODS</h3><p>Vascular protective effects of an ethanol extract of seeds from EXS (1-50 μg/mL) against tumor necrosis factor-α (TNF-α)-induced vascular inflammation were examined in human umbilical vein endothelial cells (HUVECs).</p></div><div><h3>RESULTS</h3><p>EXS significantly decreased TNF-α-induced expression of cell adhesion molecules, such as intracellular adhesion molecule-1, vascular cell adhesion molecule-1, and endothelial cell selectin, in a dose-dependent manner. Pre-treatment with EXS significantly inhibited translocation and transcriptional activity of nuclear factor-κB (NF-κB) increased by TNF-α. EXS also significantly inhibited formation of intracellular reactive oxygen species (ROS). Moreover, the vascular protective effects of EXS were linked to up-regulation of heme oxygenase-1 (HO-1) and nuclear factor E2-related factor-2 (Nrf-2) expression. EXS-induced HO-1 expression was significantly decreased in SnPP (HO-1 inhibitor)- and HO-1 siRNA-treated cells, whereas an increase was found in cobalt protoporphyrin IX (CoPP) (HO-1 inducer)-treated cells. In addition, pretreatment with EXS increased HO-1 and Nrf-2 expression under TNF-α stimulation with or without N-acetyl-L-cysteine. Furthermore, the inhibitory effects of EXS on TNF-α-induced vascular inflammation were partially reversed in SnPP- and of HO-1 siRNA-treated cells but increased by CoPP.</p></div><div><h3>CONCLUSION</h3><p>These results suggest that EXS may have important implications for prevention of vascular complications associated with vascular inflammation by inhibition of the NF-κB/ROS pathway and activation of the Nrf-2/HO-1 pathway.</p></div>","PeriodicalId":17513,"journal":{"name":"Journal of Traditional Chinese Medicine","volume":null,"pages":null},"PeriodicalIF":2.6,"publicationDate":"2018-12-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/S0254-6272(18)30979-8","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"42460618","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2018-12-15DOI: 10.1016/S0254-6272(18)30982-8
Song Pengfei , Lin Zhigang , Zhu Qingguang , Jiang Shichao , Zhang Hao , Cheng Yanbin , Sun Wuquan , Wu Zhiwei , Zhou Xin , Lü Zhizhen , Fang Min
OBJECTIVE
To demonstrate the analgesic effect of Tuina mainly from mechanically sensitive ion channels in peripheral myelinated nerve fibers.
METHODS
A total of 40 healthy and pathogen-free adult male Sprague-Dawley rats were used in the study [weight: (220.0 ± 1.4) g, Shanghai Slac Laboratory Animal Co., Ltd., Shanghai, China; license No. Shanghai ICP 05033115]. The rats were housed in cages with free access to water and food in a temperature-controlled room [(22 ± 1) °C] and 12-h/12-h light-dark cycle. Thirty-two rats were randomly divided into five groups: naive, sham, chronic compression of dorsal root ganglion (CCD), Tuina (7 d) and Tuina (21 d). CCD rat model was established via unilateral DRG compression by “L” liked steel bar. Chinese Tuina treatment was accepted once per day. Behavior monitoring of paw withdrawal threshold (PWT) and paw withdrawal latency (PWL) were tested. The expression of Piezo1 and Piezo2 in myelinated nerve fiber were analyzed by immunohistochemistry and Western-blotting.
RESULTS
There was a high expression of Piezo2 and a low expression of Piezo1 in the naive and CCD groups. In contrast, the expression of Piezo2 was down regulated and Piezo1 was increased after a period of Tuina. There was significant difference (P ≤ 0.05) between the groups.
CONCLUSION
Our findings suggest that Tuina therapy can increase the expression of Piezo2 and decrease the expression of Piezo1 in the test rats. The different changes in the expressions of Piezo1 and Piezo2 may play an important role in alleviating CCD-induced allodynia and hyperalgesia.
{"title":"Possible mechanism underlying analgesic effect of Tuina in rats may involve piezo mechanosensitive channels within dorsal root ganglia axon","authors":"Song Pengfei , Lin Zhigang , Zhu Qingguang , Jiang Shichao , Zhang Hao , Cheng Yanbin , Sun Wuquan , Wu Zhiwei , Zhou Xin , Lü Zhizhen , Fang Min","doi":"10.1016/S0254-6272(18)30982-8","DOIUrl":"10.1016/S0254-6272(18)30982-8","url":null,"abstract":"<div><h3>OBJECTIVE</h3><p>To demonstrate the analgesic effect of Tuina mainly from mechanically sensitive ion channels in peripheral myelinated nerve fibers.</p></div><div><h3>METHODS</h3><p>A total of 40 healthy and pathogen-free adult male Sprague-Dawley rats were used in the study [weight: (220.0 ± 1.4) g, Shanghai Slac Laboratory Animal Co., Ltd., Shanghai, China; license No. Shanghai ICP 05033115]. The rats were housed in cages with free access to water and food in a temperature-controlled room [(22 ± 1) °C] and 12-h/12-h light-dark cycle. Thirty-two rats were randomly divided into five groups: naive, sham, chronic compression of dorsal root ganglion (CCD), Tuina (7 d) and Tuina (21 d). CCD rat model was established <em>via</em> unilateral DRG compression by “L” liked steel bar. Chinese Tuina treatment was accepted once per day. Behavior monitoring of paw withdrawal threshold (PWT) and paw withdrawal latency (PWL) were tested. The expression of Piezo1 and Piezo2 in myelinated nerve fiber were analyzed by immunohistochemistry and Western-blotting.</p></div><div><h3>RESULTS</h3><p>There was a high expression of Piezo2 and a low expression of Piezo1 in the naive and CCD groups. In contrast, the expression of Piezo2 was down regulated and Piezo1 was increased after a period of Tuina. There was significant difference (<em>P</em> ≤ 0.05) between the groups.</p></div><div><h3>CONCLUSION</h3><p>Our findings suggest that Tuina therapy can increase the expression of Piezo2 and decrease the expression of Piezo1 in the test rats. The different changes in the expressions of Piezo1 and Piezo2 may play an important role in alleviating CCD-induced allodynia and hyperalgesia.</p></div>","PeriodicalId":17513,"journal":{"name":"Journal of Traditional Chinese Medicine","volume":null,"pages":null},"PeriodicalIF":2.6,"publicationDate":"2018-12-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/S0254-6272(18)30982-8","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"43339975","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2018-12-15DOI: 10.1016/S0254-6272(18)30991-9
Wang Chao , Wei Yun , Zhang Lin , Song Jia , Liu Ni , Fu Yanling
OBJECTIVE
To explore the blood flow differences in cun-kou (radial) artery and anterior tibial artery between normal people and patients with chronic gastritis
METHODS
Using doppler ultrasonography, blood flow charts [peak systolic velocity (VP), maximum blood flow velocity in diastole (VD), mean blood flow velocity (VM), pulse index (PI), resistance index (RI), blood vessel diameter (D), vessel volume (SV), circulation blood flow periodic time (ET)] measured by at cun-kou (radial) artery and anterior tibial artery in normal group (n = 30) and chronic gastritis group (n = 30) in department of ultrasound, Beijing Anzhen hospital, capital medical university.
RESULTS
In the doppler flow charts of the normal group, there were statistically significant differences in VD, VM, D, RI, SV, ET between cun-kou artery and anterior tibial artery (P < 0.05), and there were more statistically significant differences in PI between cun-kou artery and anterior tibial artery (P < 0.01). In the comparison of doppler flow charts in the chronic gastritis group, there were statistically significant differences in VD, VM, D, RI, SV, ET between cun-kou artery and anterior tibial artery (P < 0.05), and there were more statistically significant differences in PI between cun-kou artery and anterior tibial artery (P < 0.01). In the comparison of doppler flow charts between normal group and chronic gastritis group, there were statistically significant differences in SV, ET between normal group and chronic gastritis group (P < 0.05), and there were more statistically significant differences in PI between normal group and chronic gastritis group (P <0.01).
CONCLUSION
Based on the differences in doppler flow charts between the normal group and the chronic gastritis group, the doppler flow charts of cun-kou artery and anterior tibial artery were not only proved to be significantly different, but also provided quantitative objective indexes for the study of cun-kou artery and anterior tibial artery doppler flow charts of normal people and chronic gastritis patients. This study also proves that the doppler flow chart of the anterior tibial pulse is of great significance for the diagnosis of chronic gastritis.
{"title":"Blood flow differences in cun-kou (radial) artery and anterior tibial artery: normal people vs patients with chronic gastritis","authors":"Wang Chao , Wei Yun , Zhang Lin , Song Jia , Liu Ni , Fu Yanling","doi":"10.1016/S0254-6272(18)30991-9","DOIUrl":"10.1016/S0254-6272(18)30991-9","url":null,"abstract":"<div><h3>OBJECTIVE</h3><p>To explore the blood flow differences in cun-kou (radial) artery and anterior tibial artery between normal people and patients with chronic gastritis</p></div><div><h3>METHODS</h3><p>Using doppler ultrasonography, blood flow charts [peak systolic velocity (VP), maximum blood flow velocity in diastole (VD), mean blood flow velocity (VM), pulse index (PI), resistance index (RI), blood vessel diameter (D), vessel volume (SV), circulation blood flow periodic time (ET)] measured by at cun-kou (radial) artery and anterior tibial artery in normal group (<em>n</em> = 30) and chronic gastritis group (<em>n</em> = 30) in department of ultrasound, Beijing Anzhen hospital, capital medical university.</p></div><div><h3>RESULTS</h3><p>In the doppler flow charts of the normal group, there were statistically significant differences in VD, VM, D, RI, SV, ET between cun-kou artery and anterior tibial artery (<em>P</em> < 0.05), and there were more statistically significant differences in PI between cun-kou artery and anterior tibial artery (<em>P</em> < 0.01). In the comparison of doppler flow charts in the chronic gastritis group, there were statistically significant differences in VD, VM, D, RI, SV, ET between cun-kou artery and anterior tibial artery (<em>P</em> < 0.05), and there were more statistically significant differences in PI between cun-kou artery and anterior tibial artery (<em>P</em> < 0.01). In the comparison of doppler flow charts between normal group and chronic gastritis group, there were statistically significant differences in SV, ET between normal group and chronic gastritis group (<em>P</em> < 0.05), and there were more statistically significant differences in PI between normal group and chronic gastritis group (<em>P</em> <0.01).</p></div><div><h3>CONCLUSION</h3><p>Based on the differences in doppler flow charts between the normal group and the chronic gastritis group, the doppler flow charts of cun-kou artery and anterior tibial artery were not only proved to be significantly different, but also provided quantitative objective indexes for the study of cun-kou artery and anterior tibial artery doppler flow charts of normal people and chronic gastritis patients. This study also proves that the doppler flow chart of the anterior tibial pulse is of great significance for the diagnosis of chronic gastritis.</p></div>","PeriodicalId":17513,"journal":{"name":"Journal of Traditional Chinese Medicine","volume":null,"pages":null},"PeriodicalIF":2.6,"publicationDate":"2018-12-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1016/S0254-6272(18)30991-9","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"48828974","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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