{"title":"Nerine Latent Virus Diagnosed in Hippeastrum Bulbs in Korea","authors":"Eun Song Heo, Sun Hee Choi","doi":"10.7235/HORT.20210045","DOIUrl":null,"url":null,"abstract":"Amaryllis (Hippeastrum hybridum) bulbs at the dormant stage were used to investigate contagious virus retention. A DNA fragment containing 612 bp was amplified by reverse-transcription polymerase chain reaction with nerine latent virus (NeLV)-specific primers from amaryllis bulbs and characterized by nucleotide sequencing. Nucleotide sequences of the partial region of the NeLV coat protein gene were determined, and the sequences shared 93.9% to 98.5% identity with other NeLV isolates derived from various Amaryllidaceae species. In the phylogenetic analysis, amaryllis isolates from ‘Alfresco’ and ‘Red Lion’ cultivars used in this study were grouped with other NeLV isolates and distinctively separated from other carlavirus species. In this study, NeLV was found in amaryllis bulbs from the two cultivars. Additional key words: Amaryllidaceae, amaryllis, carlavirus, flower bulb, quarantine","PeriodicalId":17858,"journal":{"name":"Korean Journal of Horticultural Science & Technology","volume":"1 1","pages":""},"PeriodicalIF":1.0000,"publicationDate":"2021-08-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"1","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Korean Journal of Horticultural Science & Technology","FirstCategoryId":"97","ListUrlMain":"https://doi.org/10.7235/HORT.20210045","RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q3","JCRName":"HORTICULTURE","Score":null,"Total":0}
引用次数: 1
Abstract
Amaryllis (Hippeastrum hybridum) bulbs at the dormant stage were used to investigate contagious virus retention. A DNA fragment containing 612 bp was amplified by reverse-transcription polymerase chain reaction with nerine latent virus (NeLV)-specific primers from amaryllis bulbs and characterized by nucleotide sequencing. Nucleotide sequences of the partial region of the NeLV coat protein gene were determined, and the sequences shared 93.9% to 98.5% identity with other NeLV isolates derived from various Amaryllidaceae species. In the phylogenetic analysis, amaryllis isolates from ‘Alfresco’ and ‘Red Lion’ cultivars used in this study were grouped with other NeLV isolates and distinctively separated from other carlavirus species. In this study, NeLV was found in amaryllis bulbs from the two cultivars. Additional key words: Amaryllidaceae, amaryllis, carlavirus, flower bulb, quarantine
期刊介绍：
Horticultural Science and Technology (abbr. Hortic. Sci. Technol., herein ‘HST’; ISSN, 1226-8763), one of the two official journals of the Korean Society for Horticultural Science (KSHS), was launched in 1998 to provides scientific and professional publication on technology and sciences of horticultural area. As an international journal, HST is published in English and Korean, bimonthly on the last day of even number months, and indexed in ‘SCIE’, ‘SCOPUS’ and ‘CABI’. The HST is devoted for the publication of technical and academic papers and review articles on such arears as cultivation physiology, protected horticulture, postharvest technology, genetics and breeding, tissue culture and biotechnology, and other related to vegetables, fruit, ornamental, and herbal plants.
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