Stability determination of candidate reference genes in cucumber plants subjected to stresses from Phytophthora melonis

Abstract

Stable reference genes are indispensable for ensuring the fi delity of determined gene expression levels. However, the expression levels of reference genes are unable to remain constant under all possible experimental conditions. Therefore, the stability determination of reference genes is necessary in an experimental system set. In the preset study, the stability of nine cucumber candidate reference genes (CsACT, CsUBQ, CsEF1α, CsCYP, CsαTU, CsCACS, CsTIP41, CsYSL8 and CsHEL) subjected to stresses from Phytophthora melonis (P. melonis) were determined using four different analysis methods, including Delta Ct, BestKeeper, NormFinder and GeNorm. The study results revealed that CsUBQ and CsCYP were the most stable genes suitable as internal control in cucumber plants under attack by P. melonis condition. Research Article Stability determination of candidate reference genes in cucumber plants subjected to stresses from Phytophthora melonis Rui Wang, Hu Du, Qingmin Jin, Yu’e Lin, Yujuan Zhong, Chunpeng Yao, Xiaomei Xu and Tingquan Wu* Vegetable Research Institute, Guangdong Academy of Agricultural Sciences/ Guangdong Key Laboratory for New Technology Research of Vegetables, Guangzhou, 510640, China *Address for Correspondence: Tingquan Wu, Vegetable Research Institute, Guangdong Academy of Agricultural Sciences/ Guangdong Key Laboratory for New Technology Research of Vegetables, Guangzhou, 510640, China, Tel: +86 20 38469301; Email: tingquanwu@sina.com Submitted: 29 March 2019 Approved: 12 April 2019 Published: 15 April 2019 Copyright: © 2019 Wang R, et al. This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited