CFD modelling of a wave-mixed bioreactor with complex geometry and two degrees of freedom motion

IF 2.5 Q3 BIOTECHNOLOGY & APPLIED MICROBIOLOGY Frontiers in chemical engineering Pub Date : 2022-10-20 DOI:10.3389/fceng.2022.1021416
S. Seidel, R. Maschke, M. Kraume, R. Eibl, D. Eibl
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引用次数: 6

Abstract

Optimizing bioprocesses requires an in-depth understanding, from a bioengineering perspective, of the cultivation systems used. A bioengineering characterization is typically performed via experimental or numerical methods, which are particularly well-established for stirred bioreactors. For unstirred, non-rigid systems such as wave-mixed bioreactors, numerical methods prove to be problematic, as often only simplified geometries and motions can be assumed. In this work, a general approach for the numerical characterization of non-stirred cultivation systems is demonstrated using the CELL-tainer bioreactor with two degree of freedom motion as an example. In a first step, the motion is recorded via motion capturing, and a 3D model of the culture bag geometry is generated via 3D-scanning. Subsequently, the bioreactor is characterized with respect to mixing time, and oxygen transfer rate, as well as specific power input and temporal Kolmogorov length scale distribution. The results demonstrate that the CELL-tainer with two degrees of freedom outperforms classic wave-mixed bioreactors in terms of oxygen transport. In addition, it was shown that in the cell culture version of the CELL-tainer, the critical Kolmogorov length is not surpassed in any simulation.
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具有复杂几何形状和二自由度运动的波浪混合生物反应器的CFD建模
优化生物工艺需要从生物工程的角度深入了解所使用的培养系统。生物工程表征通常通过实验或数值方法进行,这对于搅拌生物反应器来说是特别成熟的。对于未扰动的非刚性系统,如波浪混合生物反应器,数值方法被证明是有问题的,因为通常只能假设简化的几何形状和运动。在这项工作中,以具有两个自由度运动的CELL容器生物反应器为例,展示了一种对非搅拌培养系统进行数值表征的通用方法。在第一步骤中,通过运动捕捉来记录运动,并且通过3D扫描来生成培养袋几何形状的3D模型。随后,对生物反应器的混合时间、氧转移速率、比功率输入和时间上的Kolmogorov长度尺度分布进行表征。结果表明,具有两个自由度的CELL容器在氧气输送方面优于经典的波浪混合生物反应器。此外,研究表明,在细胞培养版本的cell容器中,在任何模拟中都不会超过临界Kolmogorov长度。
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来源期刊
CiteScore
3.50
自引率
0.00%
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0
审稿时长
13 weeks
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