Monoclonal antibody-based immunoassay for the specific quantification of licochalcone A: an active chalcone in licorice

IF 1.7 3区农林科学 Q3 CHEMISTRY, APPLIED Food and Agricultural Immunology Pub Date : 2022-04-04 DOI:10.1080/09540105.2022.2053948

S. Krittanai, Piriyakorn Pichetpongtorn, S. Sakamoto, W. Putalun

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引用次数: 6

Abstract

ABSTRACT Licochalcone A (LicoA), which is found in the root of Chinese licorice (Glycyrrhiza inflata Batalin), has been reported as an effective anti-microbial and anti-inflammation and was approved for the treatment of rosacea and acne in clinical therapeutic. To develop a monoclonal antibody against LicoA, a mannich reaction hapten conjugate was used for immunization, followed by the hybridoma technique. Enzyme-linked immunosorbent assay (ELISA) for the quantitative determination of LicoA was developed using a constructed antibody. The assay validation results were highly specific for the target compound, but minimally cross-reactive with the structure-related substances. After optimal conditions, the detection limit was 4.32 ng/mL and the quantification limit was 6.84–107.21 ng/mL. As a result, the developed ELISA was applied to determine the concentration of LicoA in raw licorice and marketed samples. This assay will aid in the quality control of licorice and derived products, as it can be used to identify plant species and determine bioactive markers.

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甘草查尔酮A:甘草中的一种活性查尔酮

甘草查尔酮A (LicoA)是中国甘草(Glycyrrhiza inflata Batalin)根中发现的一种有效的抗微生物和抗炎症物质，已被批准用于治疗酒渣鼻和痤疮的临床治疗。采用曼尼希反应半抗原偶联物进行免疫，然后采用杂交瘤技术制备抗LicoA单克隆抗体。建立了酶联免疫吸附法(ELISA)，利用构建的抗体定量测定LicoA。实验验证结果对目标化合物具有高度特异性，但与结构相关物质的交叉反应最小。优化条件后，检测限为4.32 ng/mL，定量限为6.84 ~ 107.21 ng/mL。结果表明，所建立的酶联免疫吸附法可用于测定生甘草和市售甘草样品中LicoA的浓度。该试验将有助于甘草及其衍生产品的质量控制，因为它可用于识别植物物种和确定生物活性标记物。

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来源期刊

Food and Agricultural Immunology 农林科学-毒理学

CiteScore

5.30

自引率

6.70%

发文量

审稿时长

2 months

期刊介绍： Food and Agricultural Immunology is an international open access journal publishing original immunological research with applications in food, agricultural, environmental and veterinary science. Submissions describing the use of immunological techniques and methods are particularly welcomed. The journal aims to expand our understanding of the interactions at the interface of food and immune systems including studies on: -Development of diagnostic systems – all types of ligand-based assays, e.g. antibody, aptamer -Application of ligand-based assays for the detection or identification of molecules of interest in food science, agricultural research, veterinary investigations and clinical systems relating to food allergy or sensitivity to agricultural chemicals -Effects of food on the immune system -Studies on allergy and allergic reactions -Investigations into food allergies -Development of allergen-free food systems -Development of novel assay formats -Applications of assay systems to the monitoring of food items in relation to safety and labelling -Food quality issues, e.g. speciation, adulteration and contamination -Comparisons between different analytical techniques The journal publishes research and review articles and is essential reading for food scientists, immunologists and all those concerned with the interaction between food and immune systems.