Application of bacteriophage cocktail to control multi-drug resistant Pseudomonas aeruginosa

Abstract

Multi–drug resistant Pseudomonas aeruginosa , a significant pathogen threats the public health with high mortality. The potential of phage cocktail was designed to lysis various bacterial sources. The candidate phage was isolated from soil, river water, tap water, food and human stool which belongs to Siphoviridae and Podoviridae family. The results identified that phage cocktails inhibited, lysed multi–drug resistant Pseudomonas aeruginosa in 30 minutes with 3 to 4 log CFU reduction. In addition, these cocktails showed effectiveness to bacterial strains isolated from wide sources including environment, food, and human. This renewed approach is contributed to overcome the dramatical increase of antibiotic resistance.