Protective effects of Jiayan Kangtai granules on autoimmune thyroiditis in a rat model by modulating Th17/Treg cell balance

IF 2 4区 医学 Q2 INTEGRATIVE & COMPLEMENTARY MEDICINE Journal of Traditional Chinese Medicine Pub Date : 2018-06-01 DOI:10.1016/S0254-6272(18)30628-9
Hou Yi , Wang Tieshan , Guo Xiangyu , Sun Wen , Guo Xuan , Wu Lili , Qin Lingling , Zhang Chengfei , Liu Tonghua
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引用次数: 5

Abstract

OBJECTIVE

To investigate the protective effects of Jiayan Kangtai (JYKT) granules, consisting of 9 Chinese herbs, in a rat model of autoimmune thyroiditis (AIT), and the possible underlying mechanism.

METHODS

Female Lewis rats (6–8 weeks) were randomly apportioned to 5 groups of 10, including a normal control. AIT was induced in the untreated AIT-model group, and rats treated subsequently with daily low, medium, or high dose JYKT granules. After 12 weeks, plasma levels of thyroid autoantibodies and morphological changes in the thyroid were detected by enzyme-linked immunosorbent assay and histological examination, respectively. The presence of interleukin (IL)-6, IL23p19, and IL-2 in thyroid tissue was assessed by immunohistochemical staining. The percentages of T helper (Th)17 cells and regulatory T cells (Tregs) in the peripheral blood were analyzed by flow cytometry. Relevant levels of cytokines and proteins were examined via bead-based multiplex flow cytometry and ELISA, respectively. Expressions of genes and proteins regulated by Th17 cells and Tregs were shown by real-time PCR and Western blot.

RESULTS

Compared to the control, AIT-model rats had higher plasma concentrations of thyroid autoantibodies. The high-dose JYKT rats showed significantly lower levels of thyroid autoantibodies compared with the AIT model group. Rats in the AIT-JYKT groups also had fewer thyroid lesions and less lymphocytic infiltration, a lower percentage of Th17 cells, and a higher percentage of Tregs, compared with the AIT-model. Rats given high-dose JYKT had a significantly lower Th17/Treg ratio compared with the AIT model. Differences in plasma cytokine concentrations and relevant gene and protein expressions in the spleens of JYKT-treated rats and the AIT group suggested an association between JYKT treatment and lower Th17 cell percentage and higher Treg activity.

CONCLUSION

JYKT treatment appeared to be protective against AIT in rats, possibly via the regulation of the Th17 cell/Treg imbalance in AIT.

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佳炎康泰颗粒通过调节Th17/Treg细胞平衡对自身免疫性甲状腺炎模型大鼠的保护作用
目的探讨9味中药复方佳炎康泰颗粒对自身免疫性甲状腺炎(AIT)大鼠模型的保护作用及其可能的机制。方法6 ~ 8周龄雌性Lewis大鼠随机分为5组,每组10只,其中正常对照组1只。未处理AIT模型组大鼠诱导AIT,随后每日给予低、中、高剂量JYKT颗粒。12周后,分别用酶联免疫吸附法和组织学检查检测血浆甲状腺自身抗体水平和甲状腺形态学变化。免疫组化染色检测甲状腺组织中白细胞介素(IL)-6、IL- 23p19和IL-2的表达。流式细胞术检测外周血辅助性T细胞(Th)17和调节性T细胞(Tregs)的百分比。通过流式细胞术和酶联免疫吸附法分别检测细胞因子和蛋白的相关水平。实时荧光定量PCR和Western blot检测Th17细胞和Tregs调控的基因和蛋白的表达情况。结果与对照组相比,ait模型大鼠血浆甲状腺自身抗体浓度升高。与AIT模型组相比,高剂量JYKT大鼠甲状腺自身抗体水平明显降低。与ait模型相比,AIT-JYKT组大鼠甲状腺病变较少,淋巴细胞浸润较少,Th17细胞百分比较低,treg细胞百分比较高。与AIT模型相比,高剂量JYKT大鼠Th17/Treg比值明显降低。JYKT处理大鼠和AIT组血浆细胞因子浓度及脾脏相关基因和蛋白表达的差异表明,JYKT处理与较低的Th17细胞百分比和较高的Treg活性有关。结论jykt对大鼠AIT具有保护作用,可能是通过调节AIT中Th17细胞/Treg失衡来实现的。
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来源期刊
Journal of Traditional Chinese Medicine
Journal of Traditional Chinese Medicine INTEGRATIVE & COMPLEMENTARY MEDICINE-
CiteScore
2.40
自引率
3.80%
发文量
32269
审稿时长
2 months
期刊介绍: Journal of Traditional Chinese Medicine(JTCM) is devoted to clinical and theortical research on the use of acupuncture and Oriental medicine. The main columns include Clinical Observations, Basic Investigations, Reviews, Questions and Answers, an Expert''s Forum, and Discussions of Clinical Cases. Its key topics include acupuncture and electro-acupuncture, herbal medicine, homeopathy, masseotherapy, mind-body therapies, palliative care, and other CAM therapies.
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