Analyzing the biosynthetic potential of antimicrobial-producing actinobacteria originating from Indonesia

Q4 Environmental Science Indonesian Journal of Biotechnology Pub Date : 2021-09-30 DOI:10.22146/ijbiotech.65239

A. Utami, Pamela Apriliana, Yudi Kusnadi, D. S. Zilda, Z. Ilmiah, P. Lisdiyanti, S. Setyahadi, A. Uria

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Abstract

We investigated the biosynthetic potential of soil-associated actinobacteria originating from Indonesia, identified as Streptomyces luridus and as Streptomyces luteosporeus. Antimicrobial assays indicated inhibitory activity by both strains against the pathogen Pseudomonas aeruginosa, with S. luteosporeus particularly inhibiting the growth of Bacillus subtilis. PCR-amplification, cloning, and sequencing of ketosynthase (KS) domains of type I modular polyketide (PKS-I) and adenylation (AD) domains of non-ribosomal peptide synthetase (NRPS) indicated the diversity of KS and AD domains derived from both Indonesian Streptomyces. Further phylogenetic analysis showed that KS domains from the subclass cis-AT PKS can be classified as being a part of a loading module or an extension module, along with their predicted substrate specificity. The results suggest that both strains are a potential source of novel biosynthetic pathways. This genetic analysis approach can be used as a fast guide to obtain insight into natural product biosynthetic gene diversity in microorganisms.

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印度尼西亚产抗菌放线菌的生物合成潜力分析

我们研究了原产于印度尼西亚的土壤相关放线菌的生物合成潜力，该放线菌被鉴定为鲁氏链霉菌和黄孢链霉菌。抗菌试验表明，这两种菌株都对病原体铜绿假单胞菌具有抑制活性，其中黄曲霉尤其能抑制枯草芽孢杆菌的生长。I型模块化聚酮（PKS-I）的酮合酶（KS）结构域和非核糖体肽合成酶（NRPS）的腺苷酸化（AD）结构域的PCR扩增、克隆和测序表明来源于两种印度尼西亚链霉菌的KS和AD结构域的多样性。进一步的系统发育分析表明，来自顺式AT PKS亚类的KS结构域可以被归类为负载模块或扩展模块的一部分，以及它们预测的底物特异性。结果表明，这两种菌株都是新的生物合成途径的潜在来源。这种遗传分析方法可以作为快速指南，深入了解微生物中天然产物生物合成基因的多样性。

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