Insulinomimetic Effect of Citrullus Colocynthis Roots in STZ Challenged Rat Model

Q4 Pharmacology, Toxicology and Pharmaceutics Iranian Journal of Pharmaceutical Sciences Pub Date : 2018-07-01 DOI:10.22034/IJPS.2018.65235.1324
Sireesha Kalva, N. Fatima, R. Nerella, S. Samreen
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引用次数: 2

Abstract

Abstract Diabetes mellitus is a metabolic disease characterized by hyperglycemia resulting from either defect in insulin secretion, insulin action, or both. Postprandial hyperglycemia is a prime characteristic of diabetes mellitus and has been a focus in the therapy for diabetes. One of the therapeutic approaches which involve decreasing hyperglycemia aims at lowering blood glucose by decreasing insulin resistance, raising insulin sensitivity at the tissues and inhibiting the carbohydrate absorbance in the intestine. Plants contain different chemical constituents with potential for insulin mimetic action, decrease in insulin resistance and of α-amylase inhibitory activity may be used as therapeutic agents in the treatment of diabetes mellitus. The present study investigates the hypoglycemic activity and insulinomimetic action of aqueous and ethanolic extracts of Citrullus colocynthis by invitro α-amylase enzyme inhibition and by histopathological studies in streptozotocin (STZ) induced diabetic rats. Male Wistar rats weighing about 180- 250g were taken and divided into fifteen groups. Diabetes was induced by giving streptozotocin (STZ) (30-50mg/kg) intraperitoneally. Rats that showed blood glucose levels > 250mg/dl were selected for the study. Metformin (45mg/kg) was given as standard oral hypoglycemic agent. The aqueous and ethanolic extracts of Citrullus colocynthis (AECC and EECC) at 100mg/kg, 200mg/kg and 300mg/kg were administered to the normal and diabetic rats. The invitro α – amylase inhibitory activity was done by spectrophotometric method. Blood glucose levels were measured by glucose oxidase method. Estimation of glycogen in the lever was carried out with anthrone method. Liver and pancreas were isolated and subjected to histopathological studies. Serum insulin was monitored through chemiluminescence assay. Oral administration of both extracts showed significant inhibition of α-amylase enzyme in-vitro and decrease in blood glucose also. Glycogen and Insulin levels too were found to increase in extract treated groups which attributed for its insulinomimetic activity. The findings revealed that Citrullus colocynthis possess a very strong anti-hyperglycemic potential justifying the use of the drug for the treatment of diabetes mellitus.
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罗布麻根对STZ挑战大鼠模型的胰岛素模拟作用
糖尿病是一种以高血糖为特征的代谢性疾病,由胰岛素分泌缺陷、胰岛素作用缺陷或两者兼有引起。餐后高血糖是糖尿病的主要特征,一直是糖尿病治疗的热点。降低高血糖的治疗方法之一是通过降低胰岛素抵抗、提高组织胰岛素敏感性和抑制肠道碳水化合物吸收来降低血糖。植物中含有多种具有胰岛素模拟作用、降低胰岛素抵抗和α-淀粉酶抑制活性的化学成分,可作为治疗糖尿病的药物。本研究通过对链脲佐菌素(STZ)诱导的糖尿病大鼠α-淀粉酶的体外抑制和组织病理学研究,探讨了枸橼水提液和醇提液对糖尿病大鼠的降糖活性和胰岛素模拟作用。取体重180 ~ 250g的雄性Wistar大鼠,分为15组。腹腔注射链脲佐菌素(STZ) (30 ~ 50mg/kg)诱导糖尿病。选择血糖水平为bb0 ~ 250mg/dl的大鼠进行研究。二甲双胍(45mg/kg)作为标准口服降糖药。分别以100mg/kg、200mg/kg和300mg/kg剂量给药于正常大鼠和糖尿病大鼠。用分光光度法测定其体外α -淀粉酶抑制活性。葡萄糖氧化酶法测定血糖水平。用蒽酮法测定杠杆中的糖原。肝脏和胰腺分离并进行组织病理学研究。用化学发光法监测血清胰岛素。口服两种提取物均能显著抑制α-淀粉酶,降低血糖。糖原和胰岛素水平也被发现在提取物处理组增加,这归因于其胰岛素模拟活性。研究结果表明,西葫芦具有很强的抗高血糖潜能,证明该药物用于治疗糖尿病是合理的。
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来源期刊
Iranian Journal of Pharmaceutical Sciences
Iranian Journal of Pharmaceutical Sciences Pharmacology, Toxicology and Pharmaceutics-Pharmaceutical Science
CiteScore
0.50
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期刊介绍: Iranian Journal of Pharmaceutical Sciences (IJPS) is an open access, internationally peer-reviewed journal that seeks to publish research articles in different pharmaceutical sciences subdivisions: pharmacology and toxicology, nanotechnology, pharmaceutics, natural products, biotechnology, pharmaceutical chemistry, clinical pharmacy and other pharmacy related topics. Each issue of the journal contents 16 outstanding research articles in area of pharmaceutical sciences plus an editorial written by the IJPS editors on one of the most up to date advances topics in pharmacy. All articles published by IJPS would be permanently accessible online freely without any subscription charges. Authors of the published articles have granted the right to use and disseminate their article to third parties.
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