Pub Date : 2021-10-01DOI: 10.22034/IJPS.2020.105389.1540
K. Kumar, Gurunath Surampalli, Y. Rao, M. Ajitha
Objective: This study was planned to formulate, characterize and evaluate to establish the bioavailability of gastroretentive mucoadhesive dosage of atenolol in human subjects with possible in-vitro-in-vivo correlation. Method: In this investigation gastroretentive mucoadhesive dosage of Atenolol was formulated using HPMCK4M, chitosan and Isabgul husk by wet granulation technique. The prepared tablets were subjected to physical evaluation, in-vitro drug release and in-vivo X-ray studies, followed by the pharmacokinetic study in human volunteers. Results: All the prepared tablets showed physicochemical properties within the limits and good in-vitro mucoadhesion. Formulation F2 was selected based on the in-vitro characteristics and in-vivo radiographic studies by replacing part of the drug by adding barium sulphate. From the radiographic studies it was found that the F2 could be successfully retained in stomach for more than 6 hours. Pharmacokinetic studies showed a significant improvement in AUC0-14; 6414.93 ± 58.221 ng.h/mL (p < 0.05) when compared to reference AUC0-14; 4752.18 ± 76.759 ng.h/mL in healthy human volunteers with good invitro-invivo correlation. Conclusion: Based on in-vitro characteristics and in-vivo radiographic studies, F2 was selected as optimized gastroretentive mucoadhesive dosage form with improved bioavailability for better patient compliance and disease management.
{"title":"Formulation, characterization and evaluation to establish the bioavailability of gastroretentive mucoadhesive dosage of atenolol in human subjects with possible in-vitro-in-vivo correlation","authors":"K. Kumar, Gurunath Surampalli, Y. Rao, M. Ajitha","doi":"10.22034/IJPS.2020.105389.1540","DOIUrl":"https://doi.org/10.22034/IJPS.2020.105389.1540","url":null,"abstract":"Objective: This study was planned to formulate, characterize and evaluate to establish the bioavailability of gastroretentive mucoadhesive dosage of atenolol in human subjects with possible in-vitro-in-vivo correlation. Method: In this investigation gastroretentive mucoadhesive dosage of Atenolol was formulated using HPMCK4M, chitosan and Isabgul husk by wet granulation technique. The prepared tablets were subjected to physical evaluation, in-vitro drug release and in-vivo X-ray studies, followed by the pharmacokinetic study in human volunteers. Results: All the prepared tablets showed physicochemical properties within the limits and good in-vitro mucoadhesion. Formulation F2 was selected based on the in-vitro characteristics and in-vivo radiographic studies by replacing part of the drug by adding barium sulphate. From the radiographic studies it was found that the F2 could be successfully retained in stomach for more than 6 hours. Pharmacokinetic studies showed a significant improvement in AUC0-14; 6414.93 ± 58.221 ng.h/mL (p < 0.05) when compared to reference AUC0-14; 4752.18 ± 76.759 ng.h/mL in healthy human volunteers with good invitro-invivo correlation. Conclusion: Based on in-vitro characteristics and in-vivo radiographic studies, F2 was selected as optimized gastroretentive mucoadhesive dosage form with improved bioavailability for better patient compliance and disease management.","PeriodicalId":14582,"journal":{"name":"Iranian Journal of Pharmaceutical Sciences","volume":"16 1","pages":"53-70"},"PeriodicalIF":0.0,"publicationDate":"2021-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"48940938","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2021-10-01DOI: 10.22034/IJPS.2020.121318.1639
I. Semerjyan, G. Semerjyan, H. Semerjyan, A. Trchounian
The present study was aimed to evaluate the antibacterial potential of ethanol, methanol, acetone and aqueous extracts of some bryophytes common in Armenia (Mnium spinosum (Voit) Schwaegr, Brachythecium salebrosum (Web. et Mohr) B.S.G., Thuidiumrecognitum (Hedw) Lindb and Dicranum scoparium (Hedw). Antibacterial activity was determined using agar-well diffusion method against five bacterial species (Escherichia coli, Bacillus subtilis, Staphylococcus aureus, Salmonella enterica and S. typhimirium). The results showed that the bryophytes D. scoparium and B. salebrosum possessed high antibacterial activity in methanol extracts, whereas M. spinosum - in acetone extract. Antibacterial activity against S. aureus was comparably weaker. Ethanol extract of T. recognitum had greater antibacterial activity than the extract of M. spinosum. Of all those tested for antibacterial activity bryophytes in vitro extracts of D. scoparium have yielded the most promising results. Antibacterial activity might be caused by a high content of flavonoids in bryophytes determined. Thus, the studied bryophytes might be used to develop new antibacterial agents.
{"title":"Antibacterial Properties and Flavonoids Content of Some Mosses Common in Armenia","authors":"I. Semerjyan, G. Semerjyan, H. Semerjyan, A. Trchounian","doi":"10.22034/IJPS.2020.121318.1639","DOIUrl":"https://doi.org/10.22034/IJPS.2020.121318.1639","url":null,"abstract":"The present study was aimed to evaluate the antibacterial potential of ethanol, methanol, acetone and aqueous extracts of some bryophytes common in Armenia (Mnium spinosum (Voit) Schwaegr, Brachythecium salebrosum (Web. et Mohr) B.S.G., Thuidiumrecognitum (Hedw) Lindb and Dicranum scoparium (Hedw). Antibacterial activity was determined using agar-well diffusion method against five bacterial species (Escherichia coli, Bacillus subtilis, Staphylococcus aureus, Salmonella enterica and S. typhimirium). The results showed that the bryophytes D. scoparium and B. salebrosum possessed high antibacterial activity in methanol extracts, whereas M. spinosum - in acetone extract. Antibacterial activity against S. aureus was comparably weaker. Ethanol extract of T. recognitum had greater antibacterial activity than the extract of M. spinosum. Of all those tested for antibacterial activity bryophytes in vitro extracts of D. scoparium have yielded the most promising results. Antibacterial activity might be caused by a high content of flavonoids in bryophytes determined. Thus, the studied bryophytes might be used to develop new antibacterial agents.","PeriodicalId":14582,"journal":{"name":"Iranian Journal of Pharmaceutical Sciences","volume":"16 1","pages":"31-42"},"PeriodicalIF":0.0,"publicationDate":"2021-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"43609170","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2021-10-01DOI: 10.22034/IJPS.2020.122139.1644
Seyed Reza Naji Esfahani, Masoud Sadeghi-Dinani, F. Moazen, F. Shafiee
Breast cancer is the most common malignancy among American women and the second leading cause of cancer death after the lung cancer. For this reason, trying to find new drugs for the treatment of this disease is essential. The aim of the present study was to evaluate the cytotoxic effects of three cinnamic acid derivatives isolated from Allium ampeloprasum var. Persicum including Caffeoyl tyramine, Feruloyl tyramine, and Persicoimidate on two breast cancer cell lines, MCF-7 and BT-474. Evaluation the cytotoxic effects of mentioned purified compounds against MCF-7 and BT-474 cells was performed using MTT assay and their IC50 was determined. Finally, flow cytometery analysis on MCF-7 cells was performed and used to determine the cell death mechanism of investigated compounds. The results exhibited that the cytotoxic effects of all three compounds against breast cancer cell lines was concentration-dependent. The IC50 of Caffeoyl tyramine, Feruloyl tyramine, and Persicoimidate was determined to be as 73, 56, and 40 μg/ml for MCF-7 and 100, 54, and 37 μg/ml for BT-474 cells, respectively. So, Perscicoimidate showed the most potent cytotoxic effects against two breast cancer cells. Finally, flow cytometery analysis showed that Persicoimidate caused approximately 48% of apoptosis in concentration of 40 μg/ml. According to the tyrosine kinase inhibitory activity of cinnamic acid derivatives, these compounds has the potential of being cancer drug candidates for complementary studies on breast tumors with highly expression of EGF receptor. However, evaluation of anti-cancer effects of these compounds against other breast cancer cell lines is suggested.
{"title":"Persicoimidate isolated from Allium ampeloprasum Subsp. Persicum with Apoptotic effects against Breast cancer cell lines","authors":"Seyed Reza Naji Esfahani, Masoud Sadeghi-Dinani, F. Moazen, F. Shafiee","doi":"10.22034/IJPS.2020.122139.1644","DOIUrl":"https://doi.org/10.22034/IJPS.2020.122139.1644","url":null,"abstract":"Breast cancer is the most common malignancy among American women and the second leading cause of cancer death after the lung cancer. For this reason, trying to find new drugs for the treatment of this disease is essential. The aim of the present study was to evaluate the cytotoxic effects of three cinnamic acid derivatives isolated from Allium ampeloprasum var. Persicum including Caffeoyl tyramine, Feruloyl tyramine, and Persicoimidate on two breast cancer cell lines, MCF-7 and BT-474. Evaluation the cytotoxic effects of mentioned purified compounds against MCF-7 and BT-474 cells was performed using MTT assay and their IC50 was determined. Finally, flow cytometery analysis on MCF-7 cells was performed and used to determine the cell death mechanism of investigated compounds. The results exhibited that the cytotoxic effects of all three compounds against breast cancer cell lines was concentration-dependent. The IC50 of Caffeoyl tyramine, Feruloyl tyramine, and Persicoimidate was determined to be as 73, 56, and 40 μg/ml for MCF-7 and 100, 54, and 37 μg/ml for BT-474 cells, respectively. So, Perscicoimidate showed the most potent cytotoxic effects against two breast cancer cells. Finally, flow cytometery analysis showed that Persicoimidate caused approximately 48% of apoptosis in concentration of 40 μg/ml. According to the tyrosine kinase inhibitory activity of cinnamic acid derivatives, these compounds has the potential of being cancer drug candidates for complementary studies on breast tumors with highly expression of EGF receptor. However, evaluation of anti-cancer effects of these compounds against other breast cancer cell lines is suggested.","PeriodicalId":14582,"journal":{"name":"Iranian Journal of Pharmaceutical Sciences","volume":"16 1","pages":"43-52"},"PeriodicalIF":0.0,"publicationDate":"2021-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"49252507","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2021-10-01DOI: 10.22034/IJPS.2020.111775.1591
Roshanak Zarringhalami, P. Hanachi, Reihaneh Ramezani Tamijani
Nowadays medicinal plants have been considered as the complementary medicine in cancer treatment by researchers. Some plants possess chemical compounds which are able to inhibit the growth of cancer cells or even eliminate them through apoptosis or necrosis. In current study anticancer effect of Tilia dasystyla and Polygonatum orientale Desf extracts on AGS and SKOV-3 cancer cell lines were investigated. The cytotoxic effect of Tilia dasystyla and Polygonatum orientale Desf extracts on AGS and SKOV-3 has not been reported so far. Cancer cell lines were treated with different concentrations (100-5000 µg/ml) of T. dasystyla and P. orientale Desf methanol extracts for 24, 48, 72 hours. Cell viability of AGS and SKOV-3 cancer cells were evaluated by MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide) method. Results of the study indicate that, extracts of T. dasystyla and P. orientale Desf showed cytotoxic effect on AGS and SKOV-3 cancer cell lines. The lowest IC50 value of AGS and SKOV-3 cell lines was about 1.02 ± 0.01 mg /ml and 1.14 ± 0.17 mg / ml respectively. T. dasystyla and P. orientale Desf extracts showed cytotoxic effect on AGS and SKOV-3 cancer cell lines in time- and dose-dependent manner. Full potential of the extracts, as an option for cancer treatment, is yet to be determined by further studies on animal models and subsequent trials.
{"title":"Cytotoxic effect of Tilia dasystyla and Polygonatum orientale Desf extracts on AGS and SKOV-3 cancer cell lines","authors":"Roshanak Zarringhalami, P. Hanachi, Reihaneh Ramezani Tamijani","doi":"10.22034/IJPS.2020.111775.1591","DOIUrl":"https://doi.org/10.22034/IJPS.2020.111775.1591","url":null,"abstract":"Nowadays medicinal plants have been considered as the complementary medicine in cancer treatment by researchers. Some plants possess chemical compounds which are able to inhibit the growth of cancer cells or even eliminate them through apoptosis or necrosis. In current study anticancer effect of Tilia dasystyla and Polygonatum orientale Desf extracts on AGS and SKOV-3 cancer cell lines were investigated. The cytotoxic effect of Tilia dasystyla and Polygonatum orientale Desf extracts on AGS and SKOV-3 has not been reported so far. Cancer cell lines were treated with different concentrations (100-5000 µg/ml) of T. dasystyla and P. orientale Desf methanol extracts for 24, 48, 72 hours. Cell viability of AGS and SKOV-3 cancer cells were evaluated by MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide) method. Results of the study indicate that, extracts of T. dasystyla and P. orientale Desf showed cytotoxic effect on AGS and SKOV-3 cancer cell lines. The lowest IC50 value of AGS and SKOV-3 cell lines was about 1.02 ± 0.01 mg /ml and 1.14 ± 0.17 mg / ml respectively. T. dasystyla and P. orientale Desf extracts showed cytotoxic effect on AGS and SKOV-3 cancer cell lines in time- and dose-dependent manner. Full potential of the extracts, as an option for cancer treatment, is yet to be determined by further studies on animal models and subsequent trials.","PeriodicalId":14582,"journal":{"name":"Iranian Journal of Pharmaceutical Sciences","volume":"16 1","pages":"9-16"},"PeriodicalIF":0.0,"publicationDate":"2021-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"42481067","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2021-01-01DOI: 10.22034/IJPS.2020.109154.1567
M. Farnoud, M. Mehrpooya, Mohammad Mehdi Mahboobian, Y. Mohammadi, M. Mohammadi
Drug-drug interactions (DDIs) result from the simultaneous consumption of two or more drugs that alter the patient’s response to the initial drug. The treatment regimen in patients with kidney disease is very diverse and may be associated with several diseases that increases the risk of DDIs. This study was carried out to investigate the DDIs incidence in patients with chronic kidney disease (CKD) in the nephrology ward. This descriptive-analytical study was performed in a 4-month period in 2017. The patients’ information such as age, sex, list of drugs during hospitalization, and kidney disease stage were recorded from patients’ medical records. Drug-drug interactions were extracted using LexiComp Online. In this study 48.55% of patients were male, 51.45% were female and 53.2% of patients were in stage 5 of kidney disease. There was a significant correlation between the incidence of drug-drug interactions with stage 5 of disease (P=0.02). The highest number of interactions was categorized as type C and interaction between atorvastatin and pantoprazole was the most frequent interaction. The maximum range of prescription drugs was between 6 and 10 items by 49.7% of patients. There was a significant correlation between the incidence of drug-drug interactions and the number of prescribed drugs (P=0.03). Drug-drug interactions are common in patients with chronic kidney disease. Based on the results, the number of prescribed drugs and the stage of the disease is effective in drug-drug interactions incidence. It is possible to reduce drug complications and increase the life span of patients by recognizing drug-drug interactions.
{"title":"Evaluation of drug-drug interactions in chronic kidney disease patients: A single-center experience","authors":"M. Farnoud, M. Mehrpooya, Mohammad Mehdi Mahboobian, Y. Mohammadi, M. Mohammadi","doi":"10.22034/IJPS.2020.109154.1567","DOIUrl":"https://doi.org/10.22034/IJPS.2020.109154.1567","url":null,"abstract":"Drug-drug interactions (DDIs) result from the simultaneous consumption of two or more drugs that alter the patient’s response to the initial drug. The treatment regimen in patients with kidney disease is very diverse and may be associated with several diseases that increases the risk of DDIs. This study was carried out to investigate the DDIs incidence in patients with chronic kidney disease (CKD) in the nephrology ward. This descriptive-analytical study was performed in a 4-month period in 2017. The patients’ information such as age, sex, list of drugs during hospitalization, and kidney disease stage were recorded from patients’ medical records. Drug-drug interactions were extracted using LexiComp Online. In this study 48.55% of patients were male, 51.45% were female and 53.2% of patients were in stage 5 of kidney disease. There was a significant correlation between the incidence of drug-drug interactions with stage 5 of disease (P=0.02). The highest number of interactions was categorized as type C and interaction between atorvastatin and pantoprazole was the most frequent interaction. The maximum range of prescription drugs was between 6 and 10 items by 49.7% of patients. There was a significant correlation between the incidence of drug-drug interactions and the number of prescribed drugs (P=0.03). Drug-drug interactions are common in patients with chronic kidney disease. Based on the results, the number of prescribed drugs and the stage of the disease is effective in drug-drug interactions incidence. It is possible to reduce drug complications and increase the life span of patients by recognizing drug-drug interactions.","PeriodicalId":14582,"journal":{"name":"Iranian Journal of Pharmaceutical Sciences","volume":"16 1","pages":"81-92"},"PeriodicalIF":0.0,"publicationDate":"2021-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"68028265","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2021-01-01DOI: 10.22034/IJPS.2020.121635.1642
R. Mohebbati, Y. Kamkar-Del, F. Kazemi, H. Rakhshandeh, M. Shafei
Centella asiatica L. (C.asiatica) is a plant with a hypotensive effect. Since this effect has several mechanism(s), therefore in the current study we investigated the effects of hydroalcoholic extract of C.asiatica on cardiovascular parameters in acute hypertensive rats. Animals were divided into four groups; 1) control, 2) Angiotensin II (AngII) that received (50 ng/kg) intravenously (i.v.), 3 and 4) two groups of C.asiatica extract (CA100, and CA200 mg/kg). In treated groups, 30 min after injection of the extract, AngII was injected. Cardiovascular parameters were recorded by the power lab system after the cannulation of the femoral artery. The maximum changes (∆) of systolic blood pressure (SBP), mean arterial pressure (MAP) and heart rate (HR) were calculated and used for statistical analysis. In the AngII group, ΔSBP and ΔMAP significantly increased compared to the control group. The HR also decreased compared to the control group but it was not significant. In CA100+AngII group ΔSBP and ΔMAP significantly decreased compared to the AngII. The use of these doses of CA with AngІІ compared to AngІІ alone had a significant effect on the HR. In CA200+AngII group ΔSBP and ΔMAP significantly decreased compared to the AngII. The change of HR in this dose was not significant to the AngII. In a recent study, we concluded that C.asiatica decreased the cardiovascular responses in hypertensive rats received AngII. Also between two doses of C.asiatica did not observe any significant difference. Therefore, one of the most important hypotensive mechanism(s) of the considered plant can be the effect on Renin-angiotensin system.
{"title":"Hypotensive effect of Centella asiatica L. extract in acute Angiotensin II-induced hypertension","authors":"R. Mohebbati, Y. Kamkar-Del, F. Kazemi, H. Rakhshandeh, M. Shafei","doi":"10.22034/IJPS.2020.121635.1642","DOIUrl":"https://doi.org/10.22034/IJPS.2020.121635.1642","url":null,"abstract":"Centella asiatica L. (C.asiatica) is a plant with a hypotensive effect. Since this effect has several mechanism(s), therefore in the current study we investigated the effects of hydroalcoholic extract of C.asiatica on cardiovascular parameters in acute hypertensive rats. Animals were divided into four groups; 1) control, 2) Angiotensin II (AngII) that received (50 ng/kg) intravenously (i.v.), 3 and 4) two groups of C.asiatica extract (CA100, and CA200 mg/kg). In treated groups, 30 min after injection of the extract, AngII was injected. Cardiovascular parameters were recorded by the power lab system after the cannulation of the femoral artery. The maximum changes (∆) of systolic blood pressure (SBP), mean arterial pressure (MAP) and heart rate (HR) were calculated and used for statistical analysis. In the AngII group, ΔSBP and ΔMAP significantly increased compared to the control group. The HR also decreased compared to the control group but it was not significant. In CA100+AngII group ΔSBP and ΔMAP significantly decreased compared to the AngII. The use of these doses of CA with AngІІ compared to AngІІ alone had a significant effect on the HR. In CA200+AngII group ΔSBP and ΔMAP significantly decreased compared to the AngII. The change of HR in this dose was not significant to the AngII. In a recent study, we concluded that C.asiatica decreased the cardiovascular responses in hypertensive rats received AngII. Also between two doses of C.asiatica did not observe any significant difference. Therefore, one of the most important hypotensive mechanism(s) of the considered plant can be the effect on Renin-angiotensin system.","PeriodicalId":14582,"journal":{"name":"Iranian Journal of Pharmaceutical Sciences","volume":"16 1","pages":"71-80"},"PeriodicalIF":0.0,"publicationDate":"2021-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"68028228","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2021-01-01DOI: 10.22034/IJPS.2021.522481.1779
Alfina Ansafovna Nizamova, E. Galiakhmetova, K. Mochalov, D. Bokov, Golrokh Farnam, N. Kudashkina
Inflammatory reactions of active radicals play a primary role in many vital circumstances, and if they are not neutralized and persist in the body, many diseases will occur. Antioxidants are the very critical resources available in the body to eliminate these free radicals. Medicinal herbs have been used extensively in the treatment of diseases for centuries. Gynostemma Pentaphyllum is grown in many different locations of Europe and Asia is an adaptogenic plant that is rich in antioxidants. During this study, the antioxidant effects of Gynostemma Pentaphyllum extract are evaluated using two different methods of adrenaline autooxidation and Chemiluminescence, both patented in Russia. Our results show that the organic solvent is more suitable for the extraction of these antioxidants with the ratio of 70% ethanolic extract being yield in more extraction. This preliminary work might open a new door for future studies on the antioxidant effects of this herb grown in Russia for the treatment of inflammations and other diseases with such pathology.
{"title":"The Determination of Antioxidant Activity of Ethanol Extracts of Gynostemma Pentaphyllum","authors":"Alfina Ansafovna Nizamova, E. Galiakhmetova, K. Mochalov, D. Bokov, Golrokh Farnam, N. Kudashkina","doi":"10.22034/IJPS.2021.522481.1779","DOIUrl":"https://doi.org/10.22034/IJPS.2021.522481.1779","url":null,"abstract":"Inflammatory reactions of active radicals play a primary role in many vital circumstances, and if they are not neutralized and persist in the body, many diseases will occur. Antioxidants are the very critical resources available in the body to eliminate these free radicals. Medicinal herbs have been used extensively in the treatment of diseases for centuries. Gynostemma Pentaphyllum is grown in many different locations of Europe and Asia is an adaptogenic plant that is rich in antioxidants. During this study, the antioxidant effects of Gynostemma Pentaphyllum extract are evaluated using two different methods of adrenaline autooxidation and Chemiluminescence, both patented in Russia. Our results show that the organic solvent is more suitable for the extraction of these antioxidants with the ratio of 70% ethanolic extract being yield in more extraction. This preliminary work might open a new door for future studies on the antioxidant effects of this herb grown in Russia for the treatment of inflammations and other diseases with such pathology.","PeriodicalId":14582,"journal":{"name":"Iranian Journal of Pharmaceutical Sciences","volume":"17 1","pages":"91-98"},"PeriodicalIF":0.0,"publicationDate":"2021-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"68029569","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2021-01-01DOI: 10.22034/IJPS.2020.119862.1630
A. Derakhshani, M. Moosavi, S. Nasseri, E. Miri-Moghaddam, H. Safarpour, B. Baradaran
DMD is the prototype of muscular dystrophy in childhood. As there is currently no absolute treatment, the present study aimed to propose a new drug repositioning for DMD therapy. A microarray dataset of 16 DMD and 6 control samples were analyzed and 208 differentially expressed genes were screened. Weighted gene co-expression network analysis (WGCNA) algorithm, applied to obtain co-expressed gene networks for the establishment of transcriptional modules related to clinical and demographic data of DMD patients. Results indicated that a maximum of 11 co-expression modules is present in datasets with a varying number of genes. Turquoise module with 3334 genes was strongly correlated with collagen fibril organization as a positive regulator in DMD pathogenesis (r=0.98, p-value=2/00E-15) through which other DMD related hub-genes were identified as COL1A1, FZD10, COL1A2, CRISPLD1, FMO1, COL5A1, COL3A1, COL5A2, TP53I3, PLAGL1, RIPK2, SBF1, MLXIP, CFAP46, and TYRP1. Drug repositioning of the turquoise module identified some candidate drugs which are not presently approved for the treatment of DMD. The targets in the turquoise module indicate some drugs can greatly affect DMD's structure and function as COL1A1 with 42 fold changes was the most up-regulated gene. Furthermore, drug repositioning introduced Zoledronic acid as a potent antagonist for COL1A1.
{"title":"Systematic mining of gene co-expression network suggests a new drug repositioning for the effective treatment of Duchenne Muscular Dystrophy","authors":"A. Derakhshani, M. Moosavi, S. Nasseri, E. Miri-Moghaddam, H. Safarpour, B. Baradaran","doi":"10.22034/IJPS.2020.119862.1630","DOIUrl":"https://doi.org/10.22034/IJPS.2020.119862.1630","url":null,"abstract":"DMD is the prototype of muscular dystrophy in childhood. As there is currently no absolute treatment, the present study aimed to propose a new drug repositioning for DMD therapy. A microarray dataset of 16 DMD and 6 control samples were analyzed and 208 differentially expressed genes were screened. Weighted gene co-expression network analysis (WGCNA) algorithm, applied to obtain co-expressed gene networks for the establishment of transcriptional modules related to clinical and demographic data of DMD patients. Results indicated that a maximum of 11 co-expression modules is present in datasets with a varying number of genes. Turquoise module with 3334 genes was strongly correlated with collagen fibril organization as a positive regulator in DMD pathogenesis (r=0.98, p-value=2/00E-15) through which other DMD related hub-genes were identified as COL1A1, FZD10, COL1A2, CRISPLD1, FMO1, COL5A1, COL3A1, COL5A2, TP53I3, PLAGL1, RIPK2, SBF1, MLXIP, CFAP46, and TYRP1. Drug repositioning of the turquoise module identified some candidate drugs which are not presently approved for the treatment of DMD. The targets in the turquoise module indicate some drugs can greatly affect DMD's structure and function as COL1A1 with 42 fold changes was the most up-regulated gene. Furthermore, drug repositioning introduced Zoledronic acid as a potent antagonist for COL1A1.","PeriodicalId":14582,"journal":{"name":"Iranian Journal of Pharmaceutical Sciences","volume":"17 1","pages":"1-18"},"PeriodicalIF":0.0,"publicationDate":"2021-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"68028117","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2021-01-01DOI: 10.22034/IJPS.2020.130501.1685
F. Samiei, Enayatollah Seydi, Faezeh Dousti, A. Hayati, F. Shirazi, Jalal Pourahmad Jaktaji
The unique properties of carbon nanotubes (CNTs) have led to their use in various fields. But, the toxicity of CNTs has been reported in biological and environmental systems. The aim of this research is to study the effect of multi-wall carbon nanotubes (MWCNTs) through inhalation chamber on the mitochondrial damage and oxidative stress using the mitochondria obtained from the skin and heart. Rats were exposed to 5 mg/m3 of MWCNTs (10 nm) aerosol for 5 hours /day, 5 days/week for 2 weeks in a whole-body exposure chamber. After 2-weeks exposure, Heart and skin mitochondria were evaluated for evaluation of toxicity parameters. The results showed that nanoparticles significantly decreased mitochondrial succinate dehydrogenase (SDH) activity and increased the level of reactive oxygen species (ROS), collapse in mitochondria membrane potential (MMP), swelling in mitochondria, and cytochrome release. In conclusion, we suggested that 5 mg/m3 of MWCNTs (10 nm) induce ROS mediated cytotoxicity by directly targeting mitochondria in both skin and heart tissue.
{"title":"Assessment of the Skin and Heart Tissue Damage Following Inhalation of Carbon Nanotubes in Wistar Rats Using Isolated Mitochondria","authors":"F. Samiei, Enayatollah Seydi, Faezeh Dousti, A. Hayati, F. Shirazi, Jalal Pourahmad Jaktaji","doi":"10.22034/IJPS.2020.130501.1685","DOIUrl":"https://doi.org/10.22034/IJPS.2020.130501.1685","url":null,"abstract":"The unique properties of carbon nanotubes (CNTs) have led to their use in various fields. But, the toxicity of CNTs has been reported in biological and environmental systems. The aim of this research is to study the effect of multi-wall carbon nanotubes (MWCNTs) through inhalation chamber on the mitochondrial damage and oxidative stress using the mitochondria obtained from the skin and heart. Rats were exposed to 5 mg/m3 of MWCNTs (10 nm) aerosol for 5 hours /day, 5 days/week for 2 weeks in a whole-body exposure chamber. After 2-weeks exposure, Heart and skin mitochondria were evaluated for evaluation of toxicity parameters. The results showed that nanoparticles significantly decreased mitochondrial succinate dehydrogenase (SDH) activity and increased the level of reactive oxygen species (ROS), collapse in mitochondria membrane potential (MMP), swelling in mitochondria, and cytochrome release. In conclusion, we suggested that 5 mg/m3 of MWCNTs (10 nm) induce ROS mediated cytotoxicity by directly targeting mitochondria in both skin and heart tissue.","PeriodicalId":14582,"journal":{"name":"Iranian Journal of Pharmaceutical Sciences","volume":"17 1","pages":"69-78"},"PeriodicalIF":0.0,"publicationDate":"2021-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"68028071","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2021-01-01DOI: 10.22034/IJPS.2020.121834.1643
Siti Rofida
Natural medicine products usually consist of two to five kinds of plants that have synergistic biological activity. The responsible constituents for the therapeutic activity in plants often involve diverse chemical components. The combination of ethanol extracts of Annona squamosa L. (EEAS) and Persea americana M. (EEPA) has been shown to have antihyperlipidemic activity. To ensure the correctness and consistency of the EEAS and EEPAs combination as the antihyperlipidemic treatments, it is necessary to characterize the plant’s secondary metabolites. This study aimed to obtain the profiles of the secondary metabolites of Annona squamosa L. and Persea americana M. using the TLC-densitometry technique. Extract samples were applied to TLC plates and screened using densitometry at 254 nm. The obtained chromatogram peak was measured at the wavelength range of 200–700 nm to obtain the maximum wavelength of each peak. The results showed that EEAS had 5 peaks with rf values of 0.00, 0.61, 0.67, 0.73, and 0.87. The TLC-densitometry analysis results of EEPA showed eight peaks with rf values of 0.00, 0.11, 0.19, 0.49, 0.56, 0.71, 0.77, and 0.90. The extract chromatograms spectra showed that 2–3 peaks at the wavelength range of 300-350 nm. Chromatogram TLC-densitometry profile data on EEAS and EEPA can be used as the indicators in the raw material combinations standardization process of EEAS and EEPA as natural medicine products to treat antihyperlipidemic.
{"title":"Profile of TLC-densitometry Annona squamosa and Persea americana as Antihyperlipidemic Medicinal Raw Material","authors":"Siti Rofida","doi":"10.22034/IJPS.2020.121834.1643","DOIUrl":"https://doi.org/10.22034/IJPS.2020.121834.1643","url":null,"abstract":"Natural medicine products usually consist of two to five kinds of plants that have synergistic biological activity. The responsible constituents for the therapeutic activity in plants often involve diverse chemical components. The combination of ethanol extracts of Annona squamosa L. (EEAS) and Persea americana M. (EEPA) has been shown to have antihyperlipidemic activity. To ensure the correctness and consistency of the EEAS and EEPAs combination as the antihyperlipidemic treatments, it is necessary to characterize the plant’s secondary metabolites. This study aimed to obtain the profiles of the secondary metabolites of Annona squamosa L. and Persea americana M. using the TLC-densitometry technique. Extract samples were applied to TLC plates and screened using densitometry at 254 nm. The obtained chromatogram peak was measured at the wavelength range of 200–700 nm to obtain the maximum wavelength of each peak. The results showed that EEAS had 5 peaks with rf values of 0.00, 0.61, 0.67, 0.73, and 0.87. The TLC-densitometry analysis results of EEPA showed eight peaks with rf values of 0.00, 0.11, 0.19, 0.49, 0.56, 0.71, 0.77, and 0.90. The extract chromatograms spectra showed that 2–3 peaks at the wavelength range of 300-350 nm. Chromatogram TLC-densitometry profile data on EEAS and EEPA can be used as the indicators in the raw material combinations standardization process of EEAS and EEPA as natural medicine products to treat antihyperlipidemic.","PeriodicalId":14582,"journal":{"name":"Iranian Journal of Pharmaceutical Sciences","volume":"16 1","pages":"1-8"},"PeriodicalIF":0.0,"publicationDate":"2021-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"68028253","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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