Antifungal activity of Cinnamomum zeylanicum bark natural extract subjected to xylophagous fungi

Guilherme Valcorte, E. Santini, Maiara Talgatti, Laura Hoffmann de Oliveira, A. G. D. Silveira
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Abstract

The search for natural wood preservatives is becoming increasingly evident due to industrial preservatives presenting several environmental restrictions, such as soil, water and ecosystems contamination. Therefore, the objective of the present study is to evaluate the antifungal activity of Cinnamomum zeylanicum bark natural extract subjected to rotting fungi. In order to obtain the aqueous extract, two methods were performed with different concentrations, 50 and 100 g L -1 , using Pycnoporus sanguineus e Gloeophyllum trabeum, mycelium. In the first, we kept the C. Zeylanicum bark with distilled water for 1 h in water bath and, in the second, the same concentrations were used, however, this was kept in a recipient for 24 h. Then, the extract was subjected to sterilization along with the BDA medium in autoclave at 120oC for 20 min, they were then put in petri dishes for later evaluation of the mycelium root growth compared with the control. The fungi Pycnoporus sanguineus presented higher fungal activity, obtaining total inhibition of all treatments and extract concentrations, however, for the fungi Gloeophyllum trabeum, the 100 g L -1 concentration warmed in water bath obtained a better result than the others. Therefore, it can be concluded that the Cinnamomum zeylanicum aqueous extract presented inhibitory potential. Considering this, we suggest that more studies with this thematic should be carried out, seeking to discover new alternatives for wood preservatives that are less damaging to the environment and to mankind.
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桂皮天然提取物对食木真菌的抗真菌活性
由于工业防腐剂存在一些环境限制,如土壤、水和生态系统污染,对天然木材防腐剂的搜索越来越明显。因此,本研究的目的是评价桂皮天然提取物对腐烂真菌的抗真菌活性。为了获得水性提取物,采用不同浓度(50和100g L-1)的红脓杆菌(Pycnoporus sangeus e Gloeophyllum trabeum)菌丝体进行了两种方法。在第一种方法中,我们用蒸馏水将泽兰树皮在水浴中保存1小时,在第二种方法中使用相同的浓度,然而,将其在接受者中保存24小时。然后,将提取物与BDA培养基一起在120℃的高压釜中灭菌20分钟,然后将它们放入培养皿中,以便稍后与对照相比评估菌丝体根的生长。血红碧萝孢具有较高的真菌活性,在所有处理和提取物浓度下都能获得完全的抑制作用,而对于大叶藻来说,在水浴中加热100g L-1的浓度比其他浓度的效果更好。因此,可以得出结论,桂皮水提取物具有抑制潜力。考虑到这一点,我们建议开展更多关于这一主题的研究,寻求发现对环境和人类危害较小的木材防腐剂的新替代品。
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