COI gene-based mitochondrial DNA variation of horseshoe crab (Tachypleus gigas) reveals high genetic variation and occurrence of distinct populations in the Bay of Bengal, India, and its comparison with other populations
{"title":"COI gene-based mitochondrial DNA variation of horseshoe crab (Tachypleus gigas) reveals high genetic variation and occurrence of distinct populations in the Bay of Bengal, India, and its comparison with other populations","authors":"Sanatan Tudu, Sandeep Kumar Gupta, Bisnu Prasad Dash","doi":"10.1111/maec.12701","DOIUrl":null,"url":null,"abstract":"<p>Horseshoe crab (<i>Tachypleus gigas</i>) is an archaic group of marine species which plays a vital role in the saline ecosystem. We attempted to develop a baseline database about the ecology, phylogeography, and genetic variation among the horseshoe crab population from Odisha, India. We collected 152 samples of horseshoe crab from the coastal area of the Bay of Bengal. The generated cytochrome <i>c</i> oxidase subunit <i>I</i> gene (<i>COI</i>) sequences of <i>T</i>.<i> gigas</i> were compared with the GenBank sequences of five populations from Bangladesh, Thailand, Singapore, South China, and Malaysia. A total of 33 unique haplotypes were observed in six populations of <i>T</i>.<i> gigas</i>. Pairwise <i>F</i>-statistic distance (<i>F</i><sub>ST</sub>) between South China-India, Malaysia-India, Singapore-India, Thailand-India, and Bangladesh-India were 0.021, 0.020, 0.017, 0.015, and 0.008, respectively. It indicated that the Indian and Bangladesh populations were genetically close and distinct from the other four populations. Furthermore, the migrant per generation (Nm) was 0.16, which indicated a low gene flow among <i>T</i>.<i> gigas</i> populations. The haplotype diversity (<i>H</i>d) and nucleotide diversity (<i>π</i>) were 0.7482 and 0.00313, respectively. It signifies the ecological importance of the Indian population that would help in formulating the future strategy for the conservation of horseshoe crab.</p>","PeriodicalId":49883,"journal":{"name":"Marine Ecology-An Evolutionary Perspective","volume":"43 2","pages":""},"PeriodicalIF":1.5000,"publicationDate":"2022-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Marine Ecology-An Evolutionary Perspective","FirstCategoryId":"99","ListUrlMain":"https://onlinelibrary.wiley.com/doi/10.1111/maec.12701","RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q3","JCRName":"MARINE & FRESHWATER BIOLOGY","Score":null,"Total":0}
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Abstract
Horseshoe crab (Tachypleus gigas) is an archaic group of marine species which plays a vital role in the saline ecosystem. We attempted to develop a baseline database about the ecology, phylogeography, and genetic variation among the horseshoe crab population from Odisha, India. We collected 152 samples of horseshoe crab from the coastal area of the Bay of Bengal. The generated cytochrome c oxidase subunit I gene (COI) sequences of T. gigas were compared with the GenBank sequences of five populations from Bangladesh, Thailand, Singapore, South China, and Malaysia. A total of 33 unique haplotypes were observed in six populations of T. gigas. Pairwise F-statistic distance (FST) between South China-India, Malaysia-India, Singapore-India, Thailand-India, and Bangladesh-India were 0.021, 0.020, 0.017, 0.015, and 0.008, respectively. It indicated that the Indian and Bangladesh populations were genetically close and distinct from the other four populations. Furthermore, the migrant per generation (Nm) was 0.16, which indicated a low gene flow among T. gigas populations. The haplotype diversity (Hd) and nucleotide diversity (π) were 0.7482 and 0.00313, respectively. It signifies the ecological importance of the Indian population that would help in formulating the future strategy for the conservation of horseshoe crab.
期刊介绍：
Marine Ecology publishes original contributions on the structure and dynamics of marine benthic and pelagic ecosystems, communities and populations, and on the critical links between ecology and the evolution of marine organisms.
The journal prioritizes contributions elucidating fundamental aspects of species interaction and adaptation to the environment through integration of information from various organizational levels (molecules to ecosystems) and different disciplines (molecular biology, genetics, biochemistry, physiology, marine biology, natural history, geography, oceanography, palaeontology and modelling) as viewed from an ecological perspective. The journal also focuses on population genetic processes, evolution of life histories, morphological traits and behaviour, historical ecology and biogeography, macro-ecology and seascape ecology, palaeo-ecological reconstruction, and ecological changes due to introduction of new biota, human pressure or environmental change.
Most applied marine science, including fisheries biology, aquaculture, natural-products chemistry, toxicology, and local pollution studies lie outside the scope of the journal. Papers should address ecological questions that would be of interest to a worldwide readership of ecologists; papers of mostly local interest, including descriptions of flora and fauna, taxonomic descriptions, and range extensions will not be considered.
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