{"title":"Presence of Senecavirus A in pork sold in the United States","authors":"Vlad Petrovan, Ying Fang, R. Rowland","doi":"10.54846/jshap/1107","DOIUrl":null,"url":null,"abstract":"Objective: To estimate the prevalence and concentration of Senecavirus A (SVA) in meat sold at retail. Materials and methods: A total of 190 meat samples derived from 25 processing locations in 13 states were purchased through retail sources. The presence of virus in samples of muscle obtained from each package was assessed by polymerase chain reaction (PCR) amplification of SVA nucleic acid. A standard curve was constructed to estimate the concentration of viable virus in PCR-positive samples. Results: Two of the 190 meat samples (1.1%) were positive for SVA nucleic acid, but negative for virus by virus isolation. The amount of virus in the PCR-positive samples was estimated to be less than 14 virions/g of muscle. Implications: The low prevalence of SVA in the 190 retail-meat samples analyzed in this study, combined with a low concentration of SVA nucleic acid in the two SVA-positive samples, suggest a low risk for transmitting SVA through retail meat.","PeriodicalId":17095,"journal":{"name":"Journal of Swine Health and Production","volume":"1 1","pages":""},"PeriodicalIF":0.7000,"publicationDate":"2019-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Journal of Swine Health and Production","FirstCategoryId":"97","ListUrlMain":"https://doi.org/10.54846/jshap/1107","RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q3","JCRName":"Agricultural and Biological Sciences","Score":null,"Total":0}
引用次数: 0
Abstract
Objective: To estimate the prevalence and concentration of Senecavirus A (SVA) in meat sold at retail. Materials and methods: A total of 190 meat samples derived from 25 processing locations in 13 states were purchased through retail sources. The presence of virus in samples of muscle obtained from each package was assessed by polymerase chain reaction (PCR) amplification of SVA nucleic acid. A standard curve was constructed to estimate the concentration of viable virus in PCR-positive samples. Results: Two of the 190 meat samples (1.1%) were positive for SVA nucleic acid, but negative for virus by virus isolation. The amount of virus in the PCR-positive samples was estimated to be less than 14 virions/g of muscle. Implications: The low prevalence of SVA in the 190 retail-meat samples analyzed in this study, combined with a low concentration of SVA nucleic acid in the two SVA-positive samples, suggest a low risk for transmitting SVA through retail meat.
期刊介绍：
The Journal of Swine Health & Production (JSHAP) is an open-access and peer-reviewed journal published by the American Association of Swine Veterinarians (AASV) since 1993. The aim of the journal is the timely publication of peer-reviewed papers with a scope that encompasses the many domains of applied swine health and production, including the diagnosis, treatment, management, prevention and eradication of swine diseases, welfare & behavior, nutrition, public health, epidemiology, food safety, biosecurity, pharmaceuticals, antimicrobial use and resistance, reproduction, growth, systems flow, economics, and facility design. The journal provides a platform for researchers, veterinary practitioners, academics, and students to share their work with an international audience. The journal publishes information that contains an applied and practical focus and presents scientific information that is accessible to the busy veterinary practitioner as well as to the research and academic community. Hence, manuscripts with an applied focus are considered for publication, and the journal publishes original research, brief communications, case reports/series, literature reviews, commentaries, diagnostic notes, production tools, and practice tips. All manuscripts submitted to the Journal of Swine Health & Production are peer-reviewed.
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