Protein corona formation on epigallocatechin gallate-Au nanoparticles suppressed tumor accumulation

Q3 Materials Science JCIS open Pub Date : 2023-04-01 DOI:10.1016/j.jciso.2023.100074

Chihiro Wakayama , Sachiko Inubushi , Tomonari Kunihisa , Sachiko Mizumoto , Motoi Baba , Hirokazu Tanino , Ik Sung Cho , Tooru Ooya

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Abstract

Metal nanoparticles (NPs), such as gold NPs (AuNPs), are particularly sensitive to X-rays, and thus specific accumulation of AuNPs in a tumor would allow radiotherapy with low energy X-rays and reduced side effects. AuNPs can be generated using HAuCl₄ and the natural polyphenol epigallocatechin-3-gallate (EGCG) in the presence of citrate. Here, we generated EGCG-AuNPs in the presence of several additives and examined the accumulation of these NPs in mouse tumors following intravenous administration. EGCG-AuNPs 15 nm in diameter in the presence of sodium alginate accumulated more in tumors compared to 40-nm-diameter EGCG-AuNPs. Furthermore, the results of in vitro cellular uptake and serum protein absorption studies suggest that adsorption of 15–16 kDa serum proteins to EGCG-AuNPs suppresses accumulation in tumors. Thus, tendency to adsorb specific proteins on EGCG-AuNPs surface should be tailored for enhancing their accumulation in tumors.

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表没食子儿茶素没食子酸-金纳米粒子的蛋白冠形成抑制肿瘤积聚

金属纳米粒子(NPs)，如金纳米粒子(AuNPs)，对x射线特别敏感，因此在肿瘤中特异性积累AuNPs将允许使用低能x射线进行放射治疗，并减少副作用。在柠檬酸盐存在下，利用HAuCl4和天然多酚表没食子儿茶素-3-没食子酸酯(EGCG)可以生成AuNPs。在这里，我们在几种添加剂的存在下产生了EGCG-AuNPs，并检测了静脉给药后这些NPs在小鼠肿瘤中的积累。与直径为40纳米的EGCG-AuNPs相比，直径为15纳米的EGCG-AuNPs在海藻酸钠存在下在肿瘤中积累更多。此外，体外细胞摄取和血清蛋白吸收研究结果表明，15-16 kDa血清蛋白吸附于EGCG-AuNPs可抑制肿瘤中的积累。因此，在EGCG-AuNPs表面吸附特定蛋白的倾向应该被调整，以增强其在肿瘤中的积累。

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