Isolation, culturing and 3D bioprinting equine myoblasts

Q3 Agricultural and Biological Sciences Biological Communications Pub Date : 2022-10-10 DOI:10.21638/spbu03.2022.302
Alexandr Aimaletdinov, M. Abyzova, I. Kurilov, Alina Yuferova, Catrin Rutland, A. Rizvanov, E. Zakirova
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引用次数: 1

Abstract

Isolating and culturing myoblasts is essential for techniques such as tissue regeneration and in vitro meat production. This research describes a protocol to isolate primary myoblasts from skeletal muscle of an adult horse. The equine primary myoblasts expressed markers specific to myoblasts and had multipotent potential capabilities with differentiation into chondrocytes, adipocytes and osteoblasts in vitro. The horse myoblasts did not adhere to Cytodex 3 and grew poorly on CultiSpher-S microcarriers during in vitro cultivation. Our studies showed that the use of GelMa bioink and ionic cross-linking did not have negative effects on cell proliferation at the beginning of cultivation. However, cells showed reduced proliferative activity by day 40 following in vitro culturing. The population of primary equine myoblasts obtained from an adult individual, and propagated on microcarriers and bioink, did not meet the requirements of the regenerative veterinary and manufacturing meat in vitro regarding the quantity and quality of the cells required. Nonetheless, further optimization of the cell scaling up process, including both microcarriers and/or the bioreactor program and bioprinting, is still important.
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马成肌细胞的分离、培养和3D生物打印
分离和培养成肌细胞对于组织再生和体外肉类生产等技术至关重要。这项研究描述了一种从成年马骨骼肌中分离原代成肌细胞的方案。马原代成肌细胞表达成肌细胞特异性的标志物,并具有在体外分化为软骨细胞、脂肪细胞和成骨细胞的多能潜能。马成肌细胞不粘附于Cytodex 3,并且在体外培养过程中在CultiSpher-S微载体上生长不良。我们的研究表明,在培养开始时,使用GelMa生物墨水和离子交联对细胞增殖没有负面影响。然而,在体外培养后第40天,细胞显示出增殖活性降低。从成年个体获得并在微载体和生物墨水上繁殖的原代马成肌细胞群体在所需细胞的数量和质量方面不符合再生兽医和体外制造肉类的要求。尽管如此,进一步优化细胞放大过程,包括微载体和/或生物反应器程序和生物打印,仍然很重要。
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来源期刊
Biological Communications
Biological Communications Agricultural and Biological Sciences-Agricultural and Biological Sciences (all)
CiteScore
1.70
自引率
0.00%
发文量
21
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