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Cancer as a potential sequela of COVID-19 — should we modify 3D cell culture models accordingly? 癌症是 COVID-19 的潜在后遗症--我们是否应相应修改三维细胞培养模型?
Q3 Agricultural and Biological Sciences Pub Date : 2023-11-30 DOI: 10.21638/spbu03.2023.307
Elena Petersen, Darya Chudakova, D. Erdyneeva, Dulamsuren Zorigt, E. Shabalina, Pavel Karalkin, Igor Reshetov
COVID-19 pandemic was caused by SARS-CoV-2, a novel virus from the family Coronaviridae, firstly identified in Wuhan, China in 2019. COVID-19 remains one of the main challenges of healthcare, given growing numbers of people with COVID-19 in anamnesis, and given the long-lasting consequences and complications of this disease. Cancer is one of the most common diseases in the world, thus a big part of the population is affected by both COVID-19 and cancer. In this succinct review we refer to several recent works expressing a view that COVID-19 might be oncogenic, and describe molecular mechanisms of such phenomena. Next, we describe several tumorigenic changes in the tissue microenvironment as COVID-19 sequelae, which can potentially affect cancer pathogenesis and response of a tumor to therapy. 3D cell culture models are a “golden standard” of in vitro studies in translational oncology. To the best of our knowledge, 3D cell culture systems to study tumor behavior in the tissue microenvironment affected by COVID-19 have not been developed yet. We propose several actionable steps which can be taken to modify existing 3D cell culture models accordingly, to address the needs of translational oncology in the COVID-19 post-pandemic times.
COVID-19 大流行是由 SARS-CoV-2 引起的,SARS-CoV-2 是一种新型冠状病毒科病毒,于 2019 年在中国武汉首次发现。COVID-19 仍是医疗保健领域的主要挑战之一,因为 COVID-19 的病例越来越多,而且这种疾病会带来长期的后果和并发症。癌症是世界上最常见的疾病之一,因此很大一部分人同时受到 COVID-19 和癌症的影响。在这篇简明扼要的综述中,我们提到了最近发表的几篇关于 COVID-19 可能致癌的文章,并描述了这种现象的分子机制。接下来,我们将描述作为 COVID-19 后遗症的组织微环境中的几种致癌变化,这些变化可能会影响癌症的发病机制和肿瘤对治疗的反应。三维细胞培养模型是转化肿瘤学体外研究的 "黄金标准"。据我们所知,用于研究受 COVID-19 影响的组织微环境中肿瘤行为的三维细胞培养系统尚未开发出来。我们建议采取几个可行的步骤,对现有的三维细胞培养模型进行相应的修改,以满足 COVID-19 后大流行时期转化肿瘤学的需求。
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引用次数: 0
The preparation of samples for studying neutrophils without their isolation 无需分离即可制备用于研究中性粒细胞的样本
Q3 Agricultural and Biological Sciences Pub Date : 2023-11-30 DOI: 10.21638/spbu03.2023.302
Natalia Fedorova, Alexey Sokolov, A. Trashkov, Elena Varfolomeeva
Neutrophils are the primary cells of the innate immune system. They destroy pathogenic microorganisms carrying out the oxidative stress mechanism through phagocytosis and extracellular traps (NETs). There is no doubt about the need to study neutrophils’ functional activity, but there are some methodological problems with the nativity of conditions in such experiments. The aim of this work is to propose a method of preparing a sample of peripheral blood neutrophils for study by spectral-optical methods (such as confocal microscopy) providing an increased level of the nativity of the conditions.
中性粒细胞是先天性免疫系统的主要细胞。它们通过吞噬作用和细胞外捕获器(NET)在氧化应激机制下消灭病原微生物。研究中性粒细胞功能活动的必要性毋庸置疑,但在此类实验中,由于条件的天然性,存在一些方法上的问题。这项工作的目的是提出一种制备外周血中性粒细胞样本的方法,以便通过光谱光学方法(如共聚焦显微镜)进行研究,从而提高条件的天然性水平。
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引用次数: 0
Ethanolic extract of Aloe arborescens stimulates neonatal rat calvarial cells proliferation, migration and osteogenic differentiation 芦荟乙醇提取物可刺激新生大鼠钙化细胞增殖、迁移和成骨分化
Q3 Agricultural and Biological Sciences Pub Date : 2023-11-30 DOI: 10.21638/spbu03.2023.303
Nicolás Olegario Blanco, Valeria Gili, J. Laiuppa, Graciela Edith Santillán
The medicinal plant Aloe arborescens Miller has chemical compounds that could stimulate the activity of bone-forming cells, but no studies have been found in this regard. We evaluated the effects of different dilutions of aqueous (1/10; 1/100; 1/1000) or ethanolic (1/1000; 1/2000; 1/5000) extracts of parenchyma from A. arborescens on the viability, proliferation, migration and osteogenic differentiation of primary cell cultures from neonatal rat calvaria. In none of the conditions studied did cell viability decrease (p ≤ 0.01). Furthermore, the 1/5000 ethanolic extract dilution showed a positive effect on cell viability at 48 h and 72 h and the latter was correlated with a 27 % (p ≤ 0.01) increase in cell proliferation. Ethanolic extract significantly stimulated cell migration and cultured mineralization with respect to control, showing the maximal effect at a dilution 1/5000. Together, the results show that the A. arborescens extracts do not have toxic effects. In addition, ethanolic extract stimulates proliferation, migration and osteogenic differentiation of rat calvarial cells, suggesting a potential bone anabolic action.
药用植物芦荟(Aloe arborescens Miller)含有可刺激骨形成细胞活性的化学物质,但尚未发现这方面的研究。我们评估了不同稀释度的芦荟实质水提取物(1/10;1/100;1/1000)或乙醇提取物(1/1000;1/2000;1/5000)对新生大鼠小腿原代细胞培养物的活力、增殖、迁移和成骨分化的影响。在所有研究条件下,细胞活力均未下降(p ≤ 0.01)。此外,1/5000 乙醇提取物稀释液在 48 小时和 72 小时内对细胞存活率有积极影响,后者与细胞增殖增加 27 %(p ≤ 0.01)相关。与对照组相比,乙醇提取物能明显促进细胞迁移和培养矿化,在稀释度为 1/5000 时效果最大。这些结果表明,A. arborescens 提取物没有毒性作用。此外,乙醇提取物还能刺激大鼠腓肠肌细胞的增殖、迁移和成骨分化,表明其具有潜在的骨合成作用。
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引用次数: 0
Genetic Collections of St. Petersburg University 圣彼得堡大学遗传学收藏馆
Q3 Agricultural and Biological Sciences Pub Date : 2023-11-30 DOI: 10.21638/spbu03.2023.308
Elena Andreeva, M. Burlakovskiy, Irina Buzovkina, Elena Chekunova, Irina E. Dodueva, Elena Golubkova, A. Matveenko, Andrew Rumyantsev, Natalia Tsvetkova, S. Zadorsky, Anton Nizhnikov
Bioresource collections represent a unique source of biological diversity for research in genetics and related disciplines. The Department of Genetics and Biotechnology of St. Petersburg State University is the oldest department of genetics in Russia, founded in 1919. Throughout the entire period of development, the geneticists of St. Petersburg University have collected unique forms of plants, animals and microorganisms, on which their research was based. Many of these studies including regulation of translation termination in yeast, amyloids and prions of different organisms, genetic mapping of valuable morphological and biochemical traits to create first rye chromosome maps, and several aspects of transcription regulation in plants, had a significant novelty. The most active accumulation of collections of genetic resources at St. Petersburg State University started in the 1950-1970s when important scientific directions in the genetics of microorganisms, plants and animals, many of which continue today, were established at the department. Genetic collections are actively used in educational work for teaching dozens of educational courses. Currently, the interdisciplinary genetic collections of St. Petersburg State University consist of seven sections including genetic collections of rye, radish, garden pea, Chlamydomonas algae, Saccharomyces yeast and plasmids, Komagataella yeast, Drosophila fly. This review describes in detail the collections of the Department of Genetics and Biotechnology of St. Petersburg State University and discusses their current state, application and development prospects.
生物资源收藏是遗传学和相关学科研究的独特生物多样性来源。圣彼得堡国立大学遗传学和生物技术系成立于 1919 年,是俄罗斯历史最悠久的遗传学系。在整个发展过程中,圣彼得堡大学的遗传学家们收集了独特形态的植物、动物和微生物,并以此为基础开展研究。其中许多研究,包括酵母翻译终止的调控、不同生物的淀粉样蛋白和朊病毒、宝贵的形态和生化性状的基因图谱绘制,以创建第一个黑麦染色体图谱,以及植物转录调控的几个方面,都具有显著的新颖性。圣彼得堡国立大学最活跃的遗传资源收藏积累始于 1950-1970 年代,当时该系确立了微生物、植物和动物遗传学方面的重要科学方向,其中许多方向一直延续至今。在教育工作中,遗传学藏品被积极用于数十门教育课程的教学。目前,圣彼得堡国立大学的跨学科遗传学藏品由七个部分组成,包括黑麦、萝卜、园豌豆、衣藻、酵母和质粒、驹形酵母、果蝇的遗传学藏品。本综述详细介绍了圣彼得堡国立大学遗传学和生物技术系的藏品,并讨论了它们的现状、应用和发展前景。
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引用次数: 0
Changes in Mn availability and soil acidity in Albic Retisol limed with dolomite screenings of various sizes and doses: a long-term microfield experiment in the north-western Russia 用不同大小和剂量的白云石筛分物进行石灰化的阿尔卑斯壤土中锰的可用性和土壤酸度的变化:在俄罗斯西北部进行的一项长期微田间试验
Q3 Agricultural and Biological Sciences Pub Date : 2023-11-30 DOI: 10.21638/spbu03.2023.301
A. Litvinovich, A. Lavrishchev, Vladimir M. Bure, Vladimir Miladinović, E. Saljnikov
Liming of acidic soils is associated with various processes in the soil, including the availability of nutrients for plants. The vector and extent of these changes depend, inter alia, on the type of lime material and the doses used. Particularly, excessive liming can trigger a deficiency of manganese for crops. A long-term microfield experiment (13 test years) was carried out on Albic Retisols reclaimed with the dolomite particles of various sizes as a by-product of stone processing quarries. Ten treatments including various sized dolomite particles and their combinations, as well as traditional limestone flour on the background of NPK fertilizer were studied for the changes in soil acidity indicators (exchangeable acidity, pH; hydrolytic acidity, Hy; total acidity, Htot) and manganese availability. The amount of acidic components passing into the extract of 1N KCl was insufficient to reveal a dependency between the exchangeable acidity and the content of mobile manganese in the soil of most treatments limed with dolomite particles of various sizes. However, the relationship between the content of mobile Mn in soils and the value of hydrolytic acidity was proved by paired linear regressions. Regardless of the dose and size of dolomite particles added, the soil was highly and moderately supplied with plant available manganese during the entire study period.
酸性土壤的石灰化与土壤中的各种过程有关,包括植物养分的可用性。这些变化的载体和程度主要取决于石灰材料的类型和使用剂量。特别是,过度施用石灰会导致作物缺锰。一项长期的微田间试验(13 个试验年)是在使用石材加工采石场的副产品--各种规格的白云石颗粒开垦的阿尔卑斯 Retisols 上进行的。研究了十种处理方法,包括不同大小的白云石颗粒及其组合,以及在氮磷钾肥料背景下的传统石灰石粉,以了解土壤酸度指标(可交换酸度,pH;水解酸度,Hy;总酸度,Htot)和锰可用性的变化。在用不同大小的白云石颗粒施肥的大多数处理中,进入 1N 氯化钾提取物的酸性成分的数量不足以揭示可交换酸度与土壤中移动锰含量之间的关系。然而,成对线性回归证明了土壤中移动锰含量与水解酸度值之间的关系。在整个研究期间,无论白云石颗粒的添加剂量和大小如何,土壤中植物可利用的锰含量都很高或适中。
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引用次数: 0
Sex-dependent effects of chronic intermittent ethanol treatment in Wistar rats 慢性间歇乙醇处理对 Wistar 大鼠的性别依赖性影响
Q3 Agricultural and Biological Sciences Pub Date : 2023-11-30 DOI: 10.21638/spbu03.2023.304
Mariia Dorofeikova, Irina Antonova, G. Gromova, Anastasia Veraksa, Elena Filatova, Alexei Egorov
Multiple behavioral parameters are associated with vulnerability to alcohol dependence, but the contribution of such factors as cognitive flexibility and sex differences still require further clarification. Our goal was to assess how sex differences mediate relationships between cognitive flexibility, affective state, hierarchy and ethanol preference before and after chronic intermittent ethanol (CIE) exposure in Wistar rats. The results of our study revealed sex-dependent changes in ethanol preference and locomotor activity throughout the time. Anxiety levels were sex-dependently affected by ethanol exposure with a dramatic decrease in ethanol-exposed females. Similarly, striatal tyrosine hydroxylase expression was affected by CIE in a sex-dependent manner, with ethanol-exposed females having higher expression than males. Baseline cognitive flexibility was better in rats with higher locomotor activity and lower anxiety levels but did not correlate with ethanol preference in rats. Taken together, these results are important for understanding sex-dependent alterations in brain and behavior induced by alcohol. This provides important insights into understanding sex differences in the risk for mental disorders.
多种行为参数与酒精依赖的易感性有关,但认知灵活性和性别差异等因素的贡献仍有待进一步澄清。我们的目的是评估性别差异如何介导 Wistar 大鼠在慢性间歇性乙醇(CIE)暴露前后的认知灵活性、情感状态、层次结构和乙醇偏好之间的关系。我们的研究结果表明,乙醇偏好和运动活动在整个过程中的变化与性别有关。焦虑水平受乙醇暴露的影响与性别有关,暴露于乙醇的雌性大鼠焦虑水平急剧下降。同样,纹状体酪氨酸羟化酶的表达受 CIE 的影响也与性别有关,暴露于乙醇的雌性纹状体酪氨酸羟化酶的表达高于雄性。运动活性较高和焦虑水平较低的大鼠的基线认知灵活性较好,但与大鼠的乙醇偏好无关。综上所述,这些结果对于理解酒精诱导的大脑和行为的性别依赖性改变非常重要。这为了解精神障碍风险中的性别差异提供了重要启示。
{"title":"Sex-dependent effects of chronic intermittent ethanol treatment in Wistar rats","authors":"Mariia Dorofeikova, Irina Antonova, G. Gromova, Anastasia Veraksa, Elena Filatova, Alexei Egorov","doi":"10.21638/spbu03.2023.304","DOIUrl":"https://doi.org/10.21638/spbu03.2023.304","url":null,"abstract":"Multiple behavioral parameters are associated with vulnerability to alcohol dependence, but the contribution of such factors as cognitive flexibility and sex differences still require further clarification. Our goal was to assess how sex differences mediate relationships between cognitive flexibility, affective state, hierarchy and ethanol preference before and after chronic intermittent ethanol (CIE) exposure in Wistar rats. The results of our study revealed sex-dependent changes in ethanol preference and locomotor activity throughout the time. Anxiety levels were sex-dependently affected by ethanol exposure with a dramatic decrease in ethanol-exposed females. Similarly, striatal tyrosine hydroxylase expression was affected by CIE in a sex-dependent manner, with ethanol-exposed females having higher expression than males. Baseline cognitive flexibility was better in rats with higher locomotor activity and lower anxiety levels but did not correlate with ethanol preference in rats. Taken together, these results are important for understanding sex-dependent alterations in brain and behavior induced by alcohol. This provides important insights into understanding sex differences in the risk for mental disorders.","PeriodicalId":8998,"journal":{"name":"Biological Communications","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-11-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139201958","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Agreement of bioimpedance analysis and ultrasound scanning for fat mass, fat free mass and body fat percentage evaluation in the group of adult women 生物阻抗分析和超声波扫描在评估成年女性群体的脂肪量、无脂肪量和体脂率方面的一致性
Q3 Agricultural and Biological Sciences Pub Date : 2023-11-30 DOI: 10.21638/spbu03.2023.305
Elvira A Bondareva, Olga Parfenteva, Aleksandra Vasileva, Nikolay Kulemin, A. Gadzhiakhmedova, Olga Kovaleva, N. Khromov-Borisov
The study aims to perform an agreement analysis of bioimpedance (BIA) results obtained using АВС-02 “Medas” (Russia) and ultrasound scanning (US) using BodyMetrixTM (USA) for fat mass, fat free mass and body fat percentage in the group of females from Moscow. The study was performed with 180 female subjects 18-67 years of age. The agreement analysis conducted in the whole sample revealed a low level of agreement in estimating body fat percentage (CCC = 0.70 0.76 0.81) and fat free mass (CCC = 0.86 0.89 0.91), but agreement can be described as medium in estimating fat mass (CCC = 0.90 0.92 0.94). Then we adjusted the prediction equations and the agreement analysis was conducted again. Adjusted prediction equations improved the level of agreement to medium when estimating body fat percentage and fat free mass. Thus, the proposed equations can be used for the translation of body composition results obtained by US into the BIA data.
本研究旨在对使用АВС-02 "Medas"(俄罗斯)获得的生物阻抗(BIA)结果和使用BodyMetrixTM(美国)进行的超声波扫描(US)结果进行一致性分析,以了解莫斯科女性群体的脂肪量、无脂肪量和体脂率。研究对象为 180 名 18-67 岁的女性。对整个样本进行的一致性分析表明,在估算体脂百分比(CCC = 0.70 0.76 0.81)和无脂肪质量(CCC = 0.86 0.89 0.91)时,一致性水平较低,但在估算脂肪质量(CCC = 0.90 0.92 0.94)时,一致性可谓中等。然后,我们调整了预测方程,并再次进行了一致性分析。调整后的预测方程在估计体脂率和无脂质量时将一致性提高到了中等水平。因此,所提出的方程可用于将 US 得出的身体成分结果转化为 BIA 数据。
{"title":"Agreement of bioimpedance analysis and ultrasound scanning for fat mass, fat free mass and body fat percentage evaluation in the group of adult women","authors":"Elvira A Bondareva, Olga Parfenteva, Aleksandra Vasileva, Nikolay Kulemin, A. Gadzhiakhmedova, Olga Kovaleva, N. Khromov-Borisov","doi":"10.21638/spbu03.2023.305","DOIUrl":"https://doi.org/10.21638/spbu03.2023.305","url":null,"abstract":"The study aims to perform an agreement analysis of bioimpedance (BIA) results obtained using АВС-02 “Medas” (Russia) and ultrasound scanning (US) using BodyMetrixTM (USA) for fat mass, fat free mass and body fat percentage in the group of females from Moscow. The study was performed with 180 female subjects 18-67 years of age. The agreement analysis conducted in the whole sample revealed a low level of agreement in estimating body fat percentage (CCC = 0.70 0.76 0.81) and fat free mass (CCC = 0.86 0.89 0.91), but agreement can be described as medium in estimating fat mass (CCC = 0.90 0.92 0.94). Then we adjusted the prediction equations and the agreement analysis was conducted again. Adjusted prediction equations improved the level of agreement to medium when estimating body fat percentage and fat free mass. Thus, the proposed equations can be used for the translation of body composition results obtained by US into the BIA data.","PeriodicalId":8998,"journal":{"name":"Biological Communications","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-11-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139196518","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Natural habitats for the stable existence of wild boars (Sus scrofa) in the North 北方野猪(Sus scrofa)稳定生存的自然栖息地
Q3 Agricultural and Biological Sciences Pub Date : 2023-11-30 DOI: 10.21638/spbu03.2023.306
Igor Popov, Dmitry Starikov
Wild boar Sus scrofa populations are actively expanding northwards. Their presence in the North is closely tied to anthropogenic activities, as wild boars are either fed, or find food and suitable habitat at farmlands. However, the nature reserves of the northern part of Russia show that wild boars are able to survive on their own even in a completely natural environment. In the taiga zone, there are habitats providing for their survival in winter: wetlands in mires and around large water bodies, and dense spruce forests. Continued northwards expansion of wild boar range is likely if pressure from hunting is reduced. Modelling based on climatic variables also shows that they could potentially inhabit vast areas in the North. The existence of wild boars in the North is interrelated with other species of relatively large mammals (beavers and roe deer) and partly supports the idea of “Pleistocene rewilding” in a boreal environment, i.e., the potential to increase the variety and numbers of megafauna representatives.
野猪的数量正在积极向北扩展。野猪在北方的出现与人类活动密切相关,因为野猪要么在农田里得到喂养,要么在农田里找到食物和合适的栖息地。不过,俄罗斯北部的自然保护区表明,即使在完全自然的环境中,野猪也能独立生存。在针叶林区,有为野猪提供冬季生存条件的栖息地:沼泽和大型水体周围的湿地,以及茂密的云杉林。如果减少狩猎压力,野猪的活动范围有可能继续向北扩展。根据气候变量建立的模型也显示,野猪有可能栖息在北方的广大地区。北方野猪的存在与其他相对大型的哺乳动物物种(河狸和狍子)相互关联,并部分支持了北方环境中的 "更新世野化 "理念,即增加巨型动物代表的种类和数量的潜力。
{"title":"Natural habitats for the stable existence of wild boars (Sus scrofa) in the North","authors":"Igor Popov, Dmitry Starikov","doi":"10.21638/spbu03.2023.306","DOIUrl":"https://doi.org/10.21638/spbu03.2023.306","url":null,"abstract":"Wild boar Sus scrofa populations are actively expanding northwards. Their presence in the North is closely tied to anthropogenic activities, as wild boars are either fed, or find food and suitable habitat at farmlands. However, the nature reserves of the northern part of Russia show that wild boars are able to survive on their own even in a completely natural environment. In the taiga zone, there are habitats providing for their survival in winter: wetlands in mires and around large water bodies, and dense spruce forests. Continued northwards expansion of wild boar range is likely if pressure from hunting is reduced. Modelling based on climatic variables also shows that they could potentially inhabit vast areas in the North. The existence of wild boars in the North is interrelated with other species of relatively large mammals (beavers and roe deer) and partly supports the idea of “Pleistocene rewilding” in a boreal environment, i.e., the potential to increase the variety and numbers of megafauna representatives.","PeriodicalId":8998,"journal":{"name":"Biological Communications","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-11-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139196669","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Risk screening of non-native suckermouth armoured catfishes Pterygoplichthys spp. in the River Dinh (Vietnam) using two related decision-support tools 使用两种相关决策支持工具筛选越南定河非本地吸嘴甲鲶(Pterygoplichthys sp.)的风险
Q3 Agricultural and Biological Sciences Pub Date : 2023-06-27 DOI: 10.21638/spbu03.2023.206
Dmitry D. Zworykin, Thị Hải Yến Đinh
The invasion risk of non-native suckermouth catfishes Pterygoplichthys spp. in the River Dinh (Vietnam) was evaluated using two related decision-support tools, namely the Fish Invasiveness Screening Kit (FISK) and the Aquatic Species Invasiveness Screening Kit (AS-ISK). The results of screenings obtained independently by three assessors using both toolkits were evaluated for consistency, and compared with a screening study previously obtained for Vietnam as a wide risk assessment area. Both FISK and AS-ISK based screenings were shown to provide adequate and potentially important outcomes in terms of risk of invasiveness and were consistent amongst assessors and between toolkits. The invasion risk of Pterygoplichthys in the River Ding was found to be high and the traits of these fishes that influence their success of becoming established were considered. Some aspects in the use of FISK and AS-ISK as decision-support tools as part of the overall risk analysis of species’ invasions for large risk assessment areas are discussed.
采用鱼类入侵性筛查试剂盒(FISK)和水生物种入侵性筛查试剂盒(AS-ISK)两种相关决策支持工具,对越南定河外来吸血鲶鱼(Pterygoplichthys spp.)的入侵风险进行了评估。对三名评估人员使用两种工具包独立获得的筛查结果进行一致性评估,并与先前在越南获得的筛查研究进行比较,该研究是一个广泛的风险评估领域。基于FISK和AS-ISK的筛查在侵入性风险方面提供了充分和潜在重要的结果,并且在评估者和工具包之间是一致的。研究发现,丁河翼鱼的入侵风险较高,并对影响其成功定居的性状进行了分析。讨论了将FISK和as - isk作为决策支持工具,作为大型风险评估区物种入侵总体风险分析的一部分的一些方面。
{"title":"Risk screening of non-native suckermouth armoured catfishes Pterygoplichthys spp. in the River Dinh (Vietnam) using two related decision-support tools","authors":"Dmitry D. Zworykin, Thị Hải Yến Đinh","doi":"10.21638/spbu03.2023.206","DOIUrl":"https://doi.org/10.21638/spbu03.2023.206","url":null,"abstract":"The invasion risk of non-native suckermouth catfishes Pterygoplichthys spp. in the River Dinh (Vietnam) was evaluated using two related decision-support tools, namely the Fish Invasiveness Screening Kit (FISK) and the Aquatic Species Invasiveness Screening Kit (AS-ISK). The results of screenings obtained independently by three assessors using both toolkits were evaluated for consistency, and compared with a screening study previously obtained for Vietnam as a wide risk assessment area. Both FISK and AS-ISK based screenings were shown to provide adequate and potentially important outcomes in terms of risk of invasiveness and were consistent amongst assessors and between toolkits. The invasion risk of Pterygoplichthys in the River Ding was found to be high and the traits of these fishes that influence their success of becoming established were considered. Some aspects in the use of FISK and AS-ISK as decision-support tools as part of the overall risk analysis of species’ invasions for large risk assessment areas are discussed.","PeriodicalId":8998,"journal":{"name":"Biological Communications","volume":null,"pages":null},"PeriodicalIF":0.0,"publicationDate":"2023-06-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"43844315","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
引用次数: 0
Structural organization of epidermis in leaves of extant species of Gnetum L. (Gnetales) and Middle Jurassic Bennettitales 现存麻属植物和中侏罗世麻属植物叶片表皮的结构组织
Q3 Agricultural and Biological Sciences Pub Date : 2023-06-27 DOI: 10.21638/spbu03.2023.205
I. Bogdanova, L. Kartseva
An attempt to assess the identification and evaluation of correlations between the epidermal characters of leaves in Bennettitales and the comparison of the results with the structural organization of leaves of extant plants has been carried out. Epidermis of four species of Gnetum L. and four species of Jurassic Bennettitales (Nilssoniopteris Nathorst and Ptilophyllum Morris) was studied. The coefficients of variation for epidermal characters Gnetum range from 6.4 to 24.0 %, Ptilophyllum — 15.7–63.5 % and Nilssoniopteris — 18.0–39.9 %. In both groups of plants, the sinuosity of the tangential cell walls of the epidermal cells in the upper and lower epidermis is a stable character (Cv ≤ 18.0 %). In the Ptilophyllum and Gnetum, the length of stomata demonstrates a low level of variability (Cv ≤ 16.8 %). A significant range of the coefficients of variation in both Gnetum and Bennettitales show the number of epidermal cells per 1 mm2 of the upper and lower epidermis (17.5 % ≤ Сv ≤ 31.9 %), the area of the epidermal cells in the upper and lower epidermis (21.2 % ≤ Сv ≤ 63.5 %) and the number of stomata per 1 mm2 of epidermis (29.3 % ≤ Сv ≤ 39.9 %). Similarities in the correlation structure of the epidermal characters are revealed in correlations between sinuosity of the tangential cell walls of the epidermis, the number of stomata per 1 mm2 and their size; the length of stomata and the number of epidermal cells; stomatal index and the number of epidermal cells of the epidermis. In Gnetum, the number of differentiated stomata correlates with the number of aborted stomata. In N. angustifolia and P. caucasicum, the number of stomata correlates with the number of papillae per 1 mm2. Similarities in correlational structure of epidermis in Nilssoniopteris, Ptilophyllum, and Gnetum could be ecological adaptations or ontogenetic characters, such as the development of stomata that has been described by other researchers.
对Bennettitales叶片表皮特征之间的相关性进行了鉴定和评估,并将结果与现存植物叶片的结构组织进行了比较。对4种Gnetum L.和4种Jurassic Bennettales(Nilsoniopteris Nathorst和Ptilophyllum Morris)的表皮进行了研究。表皮性状Gnetum变异系数为6.4~24.0%,Ptilophyllum变异系数为15.7~63.5%,Nilsonopteris变异系数为18.0~39.9%。在这两组植物中,上表皮和下表皮的表皮细胞切向细胞壁的弯曲度是一个稳定的特征(Cv≤18.0%)。在Ptilophyllum和Gnetum中,气孔长度表现出低水平的变异性(Cv≤16.8%)。Gnetum和Bennettitales的显著变异系数范围显示了上下表皮每1mm2的表皮细胞数量(17.5%≤Сv≤31.9%)、上下表皮的表皮细胞面积(21.2%≤Сv≤63.5%)和每1mm2表皮的气孔数量(29.3%≤Сv≥39.9%)。表皮特征的相关结构的相似性表现在表皮切向细胞壁的弯曲度、每1mm2气孔的数量及其大小之间的相关性;气孔的长度和表皮细胞的数量;气孔指数和表皮的表皮细胞数量。在Gnetum中,分化的气孔数量与流产的气孔数量相关。在N.angustifolia和P.caucasicum中,气孔的数量与每1mm2的乳头数量相关。Nilsoniopteris、Ptilophyllum和Gnetum表皮相关结构的相似性可能是生态适应或个体发生特征,例如其他研究人员描述的气孔发育。
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引用次数: 0
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