Mey Fann Lee PhD, Yi Hsing Chen MD, PhD, Yu-Wen Chu PhD, Ping Cheng Hsieh MS, Nancy M. Wang PhD
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引用次数: 0
Abstract
Background
Many plant food allergens have been implicated in cross-reactivities and may cause repetitive pan-allergenic exposures, which increase the severity of patients’ allergic symptoms. Identification of allergenic protein levels in food allows for more precise dietary advice. This study aimed to determine LTP, profilin, and PR-10 in the 15 most popular fruits in Taiwan.
Methods
cDNA encoding profilin (Sola l 1), nonspecific lipid transfer protein (nsLTP, Sola l 3), and pathogenesis-related protein (PR-10, Sola l 4) were cloned into the pQE30, expressed in Escherichia coli, and purified. Extracts of the most popular fruits, including apple, banana, carrot, celery, dragon fruit, hami melon, jujube, kiwi, mango, melon, orange, papaya, peach, persimmon, and tomato, in Taiwan were prepared in PBS. The allergenic protein levels in these extracts were determined by immuno-dot blotting using rabbit anti-rSola l 1, 3, and 4 polyclonal antibodies.
Results
Among all 15 fruit extracts tested, peach and mango contained the highest levels of profilin, nsLTP, and PR-10. Papaya, melon, and tomato contained low but detectable levels of the three pan-allergens.
Conclusion
Our data suggest that peach and mango contain the highest levels of important pan-allergens among the 15 common fruits tested, which agrees with epidemiological data.
背景许多植物性食物过敏原与交叉反应有关，可能导致反复的泛致敏暴露，从而增加患者过敏症状的严重程度。识别食物中的致敏蛋白水平可以提供更精确的饮食建议。本研究旨在检测台湾15种最常见水果中的LTP、profilin和PR-10。方法将编码profilin（Sola l 1）、非特异性脂质转移蛋白（nsLTP，Sola l 3）和病原体相关蛋白（PR-10，Sola 1 4）的DNA克隆到pQE30中，在大肠杆菌中表达并纯化。在PBS中制备了台湾最受欢迎的水果提取物，包括苹果、香蕉、胡萝卜、芹菜、火龙果、哈密瓜、大枣、猕猴桃、芒果、甜瓜、桔子、木瓜、桃、柿子和番茄。使用兔抗rSola l 1、3和4多克隆抗体通过免疫斑点印迹法测定这些提取物中的致敏蛋白水平。结果在所检测的15种水果提取物中，桃和芒果的profilin、nsLTP和PR-10含量最高。木瓜、甜瓜和番茄含有较低但可检测到的三种泛过敏原。结论我们的数据表明，在测试的15种常见水果中，桃和芒果含有最高水平的重要泛过敏原，这与流行病学数据一致。
期刊介绍：
Allergo Journal International is the official Journal of the German Society for Applied Allergology (AeDA) and the Austrian Society for Allergology and Immunology (ÖGAI). The journal is a forum for the communication and exchange of ideas concerning the various aspects of allergy (including related fields such as clinical immunology and environmental medicine) and promotes German allergy research in an international context. The aim of Allergo Journal International is to provide state of the art information for all medical and scientific disciplines that deal with allergic, immunological and environmental diseases. Allergo Journal International publishes original articles, reviews, short communications, case reports, and letters to the editor. The articles cover topics such as allergic, immunological and environmental diseases, the latest developments in diagnosis and therapy as well as current research work concerning antigens and allergens and aspects related to occupational and environmental medicine. In addition, it publishes clinical guidelines and position papers approved by expert panels of the German, Austrian and Swiss Allergy Societies.
All submissions are reviewed in single-blind fashion by at least two reviewers.
Originally, the journal started as a German journal called Allergo Journal back in 1992. Throughout the years, English articles amounted to a considerable portion in Allergo Journal. This was one of the reasons to extract the scientific content and publish it in a separate journal. Hence, Allergo Journal International was born and now is the international continuation of the original German journal. Nowadays, all original content is published in Allergo Journal International first. Later, selected manuscripts will be translated and published in German and included in Allergo Journal.
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