Production of pea hull soluble fiber-derived oligosaccharides using subcritical water with carboxylic acids

IF 3.4 3区工程技术 Q2 CHEMISTRY, PHYSICAL Journal of Supercritical Fluids Pub Date : 2021-12-01 DOI:10.1016/j.supflu.2021.105349

Carla S. Valdivieso Ramirez, Feral Temelli, Marleny D.A. Saldaña

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引用次数: 11

Abstract

This study aimed to evaluate a two-stage sequential approach based on subcritical water technology and the use of citric and malic acids to first extract soluble polysaccharides from pea hull fiber and then hydrolyze them into oligosaccharides. Subcritical water extraction with aqueous citric acid (120 °C/50 bar/30 min) yielded 11.8% pea soluble polysaccharides comprised of pectic fragments (17.4% galacturonic acid) with an esterification degree of 50.9%. Pectic fragments were estimated as rhamnogalacturonan-I, xylogalacturonan and xyloglucan. The HPSEC/RID and HILIC-ELSD analyses showed that subcritical water hydrolysis of pectic fragments into oligosaccharides was favored by aqueous citric and malic acids (0.2%) at 100 bar between 125 °C and 155 °C. The yields of released gluco-oligosaccharides (2–6 DP) were 6.1% and 20.4% at 125 °C and 135 °C/100 bar/120 min, respectively. The main hydrolysis mechanism exhibited a stepwise pattern, where rhamnogalacturonan-I side chains were cleaved first followed by their breakdown into reduced molecular weight compounds and oligosaccharides.

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利用亚临界水和羧酸生产豌豆壳可溶性纤维低聚糖

本研究以亚临界水技术为基础，采用柠檬酸和苹果酸为原料，先从豌豆壳纤维中提取可溶性多糖，再将其水解为低聚糖。用柠檬酸水溶液(120°C/50 bar/30 min)进行亚临界水萃取，得到由果胶片段(17.4%半乳糖醛酸)组成的11.8%豌豆可溶性多糖，酯化度为50.9%。果胶片段估计为鼠李糖半乳葡聚糖- 1、木半乳葡聚糖和木葡聚糖。HPSEC/RID和HILIC-ELSD分析表明，在125°C和155°C之间100 bar的温度下，柠檬酸和苹果酸(0.2%)有利于果胶片段亚临界水水解成低聚糖。在125°C和135°C/100 bar/120 min条件下，低聚糖(2-6 DP)的提取率分别为6.1%和20.4%。鼠李糖半乳糖醛酸- 1的主要水解机制为阶梯式，侧链首先被裂解，然后分解为低分子量化合物和低聚糖。

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来源期刊

Journal of Supercritical Fluids 工程技术-工程：化工

CiteScore

7.60

自引率

10.30%

发文量

236

审稿时长

56 days

期刊介绍： The Journal of Supercritical Fluids is an international journal devoted to the fundamental and applied aspects of supercritical fluids and processes. Its aim is to provide a focused platform for academic and industrial researchers to report their findings and to have ready access to the advances in this rapidly growing field. Its coverage is multidisciplinary and includes both basic and applied topics. Thermodynamics and phase equilibria, reaction kinetics and rate processes, thermal and transport properties, and all topics related to processing such as separations (extraction, fractionation, purification, chromatography) nucleation and impregnation are within the scope. Accounts of specific engineering applications such as those encountered in food, fuel, natural products, minerals, pharmaceuticals and polymer industries are included. Topics related to high pressure equipment design, analytical techniques, sensors, and process control methodologies are also within the scope of the journal.