Guava seed polysaccharides ameliorate the inflammatory status in PC-3 xenograft mice

IF 4.6 Q1 CHEMISTRY, APPLIED Food Hydrocolloids for Health Pub Date : 2022-12-01 DOI:10.1016/j.fhfh.2022.100068
Hsiao-Chien Lin , Jin-Yuarn Lin
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引用次数: 3

Abstract

This work evaluates the effects of guava seed polysaccharide (GSPS) on inflammatory status in the experiment mice under PC-3 human prostate cancer stress for 4 weeks. Dietary control (DC group, 0 mg GSPS/kg BW/day), 50 (GL group), 250 (GM group) as well as 500 mg GSPS/kg BW/day (GH group) by gavage, positive control (PC group, 1 mg paclitaxel/week, i.p.) and non-treatment control (NTC group, normal mice without treatment) were designed in the experiment. Changes in tumor weights, serum non-specific antibody titers, Th1/Th2 cytokines secreted by splenocytes and pro-/anti-inflammatory cytokines secreted by peritoneal macrophages from the experiment mice were measured. As a result, GH group significantly (P < 0.05) decreased serum (IgM+IgA)/IgG ratio, but GL and GM groups slightly increased (IgM+IgA)/IgG ratios compared to that of DC group, demonstrating a differential effect on the serum antibody profile. Even though GSPS administration just slightly (P > 0.05) enhanced the effect to decrease tumor weights, there was a significantly (P < 0.05) negative correlation between tumor weights and (IgM+IgA)/IgG antibody titer ratios. GSPS administrations enhanced a Th2-inclined immune balance in splenocytes and anti-inflammatory responses in peritoneal macrophages in the experiment mice. Pro-inflammatory cytokine secretions by peritoneal macrophages were positively correlated with PC-3 tumor weights in vivo, inferring that anti-inflammatory cytokine secretions increased in vivo may decrease PC-3 tumor weight. Taken together, our results evidence that GSPS administrations ameliorated the inflammatory status in the experiment mice under PC-3 prostate cancer stress via regulating Th1/Th2 and pro-/anti-inflammatory cytokine secretion profiles toward Th2-inclined and anti-inflammatory immunity.

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番石榴籽多糖可改善PC-3异种移植小鼠的炎症状态
本研究评价番石榴籽多糖(GSPS)对PC-3人前列腺癌应激4周实验小鼠炎症状态的影响。试验设饲粮灌胃对照组(DC组,0 mg GSPS/kg BW/d)、50 mg GSPS/kg BW/d (GL组)、250 mg GSPS/kg BW/d (GH组)、阳性对照组(PC组,1 mg紫杉醇/周,ig)和非处理对照组(NTC组,未处理的正常小鼠)。测定小鼠肿瘤重量、血清非特异性抗体滴度、脾细胞分泌的Th1/Th2细胞因子和腹膜巨噬细胞分泌的促炎/抗炎细胞因子的变化。结果,GH组显著(P <0.05)降低血清(IgM+IgA)/IgG比值,但与DC组相比,GL和GM组略有升高(IgM+IgA)/IgG比值,说明对血清抗体分布的影响是不同的。即使GSPS管理只是稍微(P >0.05)增强了降低肿瘤重量的效果,显著(P <(0.05)肿瘤质量与(IgM+IgA)/IgG抗体滴度比呈负相关。GSPS增强了实验小鼠脾细胞中th2倾斜的免疫平衡和腹膜巨噬细胞的抗炎反应。腹膜巨噬细胞分泌促炎细胞因子与体内PC-3肿瘤重量呈正相关,推测体内抗炎细胞因子分泌增加可能降低PC-3肿瘤重量。综上所述,我们的研究结果表明,GSPS通过调节Th1/Th2和促/抗炎细胞因子分泌谱,改善PC-3前列腺癌应激小鼠的炎症状态,从而促进Th2倾斜和抗炎免疫。
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61 days
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