{"title":"Guava seed polysaccharides ameliorate the inflammatory status in PC-3 xenograft mice","authors":"Hsiao-Chien Lin , Jin-Yuarn Lin","doi":"10.1016/j.fhfh.2022.100068","DOIUrl":null,"url":null,"abstract":"<div><p>This work evaluates the effects of guava seed polysaccharide (GSPS) on inflammatory status in the experiment mice under PC-3 human prostate cancer stress for 4 weeks. Dietary control (DC group, 0 mg GSPS/kg BW/day), 50 (GL group), 250 (GM group) as well as 500 mg GSPS/kg BW/day (GH group) by gavage, positive control (PC group, 1 mg paclitaxel/week, i.p.) and non-treatment control (NTC group, normal mice without treatment) were designed in the experiment. Changes in tumor weights, serum non-specific antibody titers, Th1/Th2 cytokines secreted by splenocytes and pro-/anti-inflammatory cytokines secreted by peritoneal macrophages from the experiment mice were measured. As a result, GH group significantly (<em>P</em> < 0.05) decreased serum (IgM+IgA)/IgG ratio, but GL and GM groups slightly increased (IgM+IgA)/IgG ratios compared to that of DC group, demonstrating a differential effect on the serum antibody profile. Even though GSPS administration just slightly (<em>P</em> > 0.05) enhanced the effect to decrease tumor weights, there was a significantly (<em>P</em> < 0.05) negative correlation between tumor weights and (IgM+IgA)/IgG antibody titer ratios. GSPS administrations enhanced a Th2-inclined immune balance in splenocytes and anti-inflammatory responses in peritoneal macrophages in the experiment mice. Pro-inflammatory cytokine secretions by peritoneal macrophages were positively correlated with PC-3 tumor weights <em>in vivo</em>, inferring that anti-inflammatory cytokine secretions increased <em>in vivo</em> may decrease PC-3 tumor weight. Taken together, our results evidence that GSPS administrations ameliorated the inflammatory status in the experiment mice under PC-3 prostate cancer stress via regulating Th1/Th2 and pro-/anti-inflammatory cytokine secretion profiles toward Th2-inclined and anti-inflammatory immunity.</p></div>","PeriodicalId":12385,"journal":{"name":"Food Hydrocolloids for Health","volume":"2 ","pages":"Article 100068"},"PeriodicalIF":4.6000,"publicationDate":"2022-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.sciencedirect.com/science/article/pii/S2667025922000152/pdfft?md5=94ec3fec3d304b3235ad47794f5395ca&pid=1-s2.0-S2667025922000152-main.pdf","citationCount":"3","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Food Hydrocolloids for Health","FirstCategoryId":"1085","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S2667025922000152","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"CHEMISTRY, APPLIED","Score":null,"Total":0}
引用次数: 3
Abstract
This work evaluates the effects of guava seed polysaccharide (GSPS) on inflammatory status in the experiment mice under PC-3 human prostate cancer stress for 4 weeks. Dietary control (DC group, 0 mg GSPS/kg BW/day), 50 (GL group), 250 (GM group) as well as 500 mg GSPS/kg BW/day (GH group) by gavage, positive control (PC group, 1 mg paclitaxel/week, i.p.) and non-treatment control (NTC group, normal mice without treatment) were designed in the experiment. Changes in tumor weights, serum non-specific antibody titers, Th1/Th2 cytokines secreted by splenocytes and pro-/anti-inflammatory cytokines secreted by peritoneal macrophages from the experiment mice were measured. As a result, GH group significantly (P < 0.05) decreased serum (IgM+IgA)/IgG ratio, but GL and GM groups slightly increased (IgM+IgA)/IgG ratios compared to that of DC group, demonstrating a differential effect on the serum antibody profile. Even though GSPS administration just slightly (P > 0.05) enhanced the effect to decrease tumor weights, there was a significantly (P < 0.05) negative correlation between tumor weights and (IgM+IgA)/IgG antibody titer ratios. GSPS administrations enhanced a Th2-inclined immune balance in splenocytes and anti-inflammatory responses in peritoneal macrophages in the experiment mice. Pro-inflammatory cytokine secretions by peritoneal macrophages were positively correlated with PC-3 tumor weights in vivo, inferring that anti-inflammatory cytokine secretions increased in vivo may decrease PC-3 tumor weight. Taken together, our results evidence that GSPS administrations ameliorated the inflammatory status in the experiment mice under PC-3 prostate cancer stress via regulating Th1/Th2 and pro-/anti-inflammatory cytokine secretion profiles toward Th2-inclined and anti-inflammatory immunity.