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Green synthesis of silver nanoparticles from mulberry leaf through hot melt extrusion: Enhanced antioxidant, antibacterial, anti-inflammatory, antidiabetic, and anticancer properties 通过热熔挤压从桑叶中绿色合成银纳米粒子:增强抗氧化、抗菌、抗炎、抗糖尿病和抗癌特性
IF 4.6 Q1 CHEMISTRY, APPLIED Pub Date : 2024-10-10 DOI: 10.1016/j.fhfh.2024.100184
In this study, mulberry leaf (ML) extract, along with ML processed by hot melt extrusion (HME) (HML), and ML processed by HME with biopolymer (HMLE), were utilized for the eco-friendly synthesis of silver nanoparticles (AgNPs). The physicochemical properties of AgNPs synthesized from ML, HML, and HMLE extracts were characterized using UV–Vis spectrophotometry, zeta potential analysis, transmission electron microscopy (TEM), dynamic light scattering (DLS), energy dispersive spectroscopy (EDS), X-ray diffraction (XRD), and Fourier transform infrared spectroscopy (FT-IR). HME processing enhanced the active ingredients in the ML extract and increased the reduction efficiency of Ag ions. In the ABTS radical scavenging activity assay, AgNPs synthesized from HMLE (F3) exhibited the highest antioxidant activity with the lowest IC50 value. F3 also demonstrated the lowest minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) against Escherichia coli and Staphylococcus aureus. In the disk diffusion test, F3 showed the largest zone of inhibition, indicating the highest antimicrobial activity. In the anti-inflammatory assay using albumin inhibition, F3 achieved the highest inhibition rate, followed by the HMLE extract. In contrast, the extract group exhibited no activity in the antidiabetic test, while the AgNPs group showed the highest antidiabetic activity in F3. Additionally, F3 demonstrated the most potent anticancer activity against breast cancer cells, resulting in the lowest cell viability. Overall, this study suggests that AgNPs synthesized using ML extract enhanced via HME processing exhibit superior physiological activities compared to those synthesized using unprocessed ML extract.
本研究利用桑叶(ML)提取物、经热熔挤压(HME)处理的 ML(HML)和经 HME 与生物聚合物(HMLE)处理的 ML(HML),以生态友好的方式合成银纳米粒子(AgNPs)。利用紫外可见分光光度法、zeta 电位分析、透射电子显微镜(TEM)、动态光散射(DLS)、能量色散光谱(EDS)、X 射线衍射(XRD)和傅立叶变换红外光谱(FT-IR)对用 ML、HML 和 HMLE 提取物合成的 AgNPs 的理化性质进行了表征。HME 处理增强了 ML 提取物中的活性成分,并提高了银离子的还原效率。在 ABTS 自由基清除活性实验中,由 HMLE(F3)合成的 AgNPs 表现出最高的抗氧化活性和最低的 IC50 值。F3 对大肠杆菌和金黄色葡萄球菌的最低抑菌浓度(MIC)和最低杀菌浓度(MBC)也最低。在盘扩散试验中,F3 的抑菌区最大,表明其抗菌活性最高。在使用白蛋白抑制的抗炎试验中,F3 的抑制率最高,其次是 HMLE 提取物。相比之下,提取物组在抗糖尿病试验中没有表现出活性,而 AgNPs 组在 F3 中表现出最高的抗糖尿病活性。此外,F3 对乳腺癌细胞的抗癌活性最强,细胞存活率最低。总之,这项研究表明,与使用未加工的 ML 提取物合成的 AgNPs 相比,使用通过 HME 加工增强的 ML 提取物合成的 AgNPs 具有更高的生理活性。
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引用次数: 0
Silkworm pupae protein-based film incorporated with Catharanthus roseus leaf extract-based nanoparticles enhanced the lipid stability and microbial quality of cheddar cheese 基于蚕蛹蛋白的薄膜与基于长春花叶提取物的纳米颗粒相结合,提高了切达干酪的脂质稳定性和微生物质量
IF 4.6 Q1 CHEMISTRY, APPLIED Pub Date : 2024-08-06 DOI: 10.1016/j.fhfh.2024.100183

The study aimed to develop a silkworm pupae protein-based film for enhancing the lipid oxidative and microbial stability of cheddar cheese. The bioactive properties were imparted to the silkworm pupae protein-based film using an optimum level (2.0%) of Catharanthus roseus leaf extract-based nanoparticles (Cat-Ros-NPs) synthesised following a green method. The cheese samples were packaged within the treated film (T2, containing 2.0% Cat-Ros-NPs) and compared with control samples [control (cheese samples without any film), T0 (cheese samples within the films without any bioactive agent) and T1 {cheese samples within the films containing 2.0% C. roseus leaf extract (Cat-Ros-Ext)}] during 90 days trial (4±1 °C). The addition of bioactive agents (Cat-Ros-Ext or Cat-Ros-NPs) increased the thickness (µm) as well as density (g/ml) of the film, thereby decreasing the transmittance (%), solubility (%), moisture content (%), and water-vapour transmission rate (mg/mt2). Both the bioactive agents increased the redness (a*) and yellowness (b*) whereas decreased the brightness (L*) of the film. The films enhanced the antioxidant and antimicrobial properties of the enclosed cheese samples during storage and the highest values were recorded for the samples packed within T2 films. The cheese samples packaged within T2 and T1 films showed significantly lower values for lipid oxidation and microbial counts. This positive effect of the films (T2 and T1) was also recorded on protein oxidation (total-carbonyl content) after day 30 and sensory quality after day 60. Our results indicate the successful use of silkworm pupae protein for the development of bioactive packaging for cheddar cheese.

该研究旨在开发一种基于蚕蛹蛋白的薄膜,以提高切达干酪的脂质氧化稳定性和微生物稳定性。蚕蛹蛋白薄膜的生物活性特性是通过采用绿色方法合成的最佳含量(2.0%)的长春花叶提取物纳米粒子(Cat-Ros-NPs)来实现的。在 90 天的试验期间(4±1 °C),将奶酪样品包装在经过处理的薄膜中(T2,含有 2.0% 的 Cat-Ros-NPs),并与对照样品[对照组(不含任何薄膜的奶酪样品)、T0(不含任何生物活性剂的薄膜中的奶酪样品)和 T1{含有 2.0% 的蔷薇叶提取物(Cat-Ros-Ext)的薄膜中的奶酪样品}]进行比较。添加生物活性剂(Cat-Ros-Ext 或 Cat-Ros-NPs)增加了薄膜的厚度(微米)和密度(克/毫升),从而降低了透光率(%)、溶解度(%)、含水量(%)和水蒸气透过率(毫克/平方米)。两种生物活性剂都增加了薄膜的红度(a*)和黄度(b*),同时降低了亮度(L*)。薄膜增强了贮藏期间封闭奶酪样品的抗氧化和抗菌性能,T2 薄膜中包装的样品的抗氧化和抗菌性能值最高。包装在 T2 和 T1 薄膜中的奶酪样品的脂质氧化值和微生物数量都明显较低。薄膜(T2 和 T1)对第 30 天后的蛋白质氧化(总羰基含量)和第 60 天后的感官质量也有积极影响。我们的研究结果表明,蚕蛹蛋白可成功用于开发切达奶酪的生物活性包装。
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引用次数: 0
Re-processing of pharmaceutical herb residues using isolated probiotics from plant sources and their beneficial effects on diarrhea 利用从植物中分离的益生菌再加工药草残留物及其对腹泻的有益作用
IF 4.6 Q1 CHEMISTRY, APPLIED Pub Date : 2024-06-20 DOI: 10.1016/j.fhfh.2024.100181
Samima Yeasmin , Abu Naser Md Nayeem , Anjumanara khatun , ABM Ashraful , Muhsi Faiaz , Shumaia Parvin , Most. Afia Akhtar , Md Aziz Abdur Rahman , Md Abu Reza , Athanasios Alexiou , Marios Papadakis , Nermeen N. Welson , Mohamed H. Mahmoud , Gaber El-Saber Batiha , Ronok Zahan

This study aims to use hot aqueous extract of herbal residue (HRE) to enhance bacterial growth and possess anti-diarrheal effects. In this study, lactobacillus species L. brevis (SAM-1), Lactobacillus plantarum (SAM-2), and Lactobacillus herbinensis (SAM-3) were isolated from date palm sap (collected in winter season). Square Pharmaceuticals PLC, Bangladesh provided herbal residues ‘Adovas’ which is non-sedating herbal cough syrup with sixteen common herbs including Adhatoda vasica. In our observation, HRE increased the number of colonies in MRS media. In the anti-diarrheal study by castor oil and magnesium sulphate-induced diarrheal mouse model, SAM-1 and 2 with or without HRE showed almost similar results. After initial morphological characterization, tests such as resistance to low pH, bile salt and survival capability in gastric simulated fluid (GSF) were performed to confirm them as a probiotic candidate. All three isolates were gram-positive bacteria and could grow in a mesophilic range of temperatures. The isolates were catalase-negative and were able to coagulate milk after overnight incubation. As the isolates exhibited resistance to low pH and could tolerate bile salts, they may survive in the stomach and intestine, thus making them a promising probiotic candidate. The isolated probiotics and HRE inhibited diarrheal and restored the body's electrolytes. Interestingly, SAM-2 showed higher efficacy than the standard drug (Loperamide), while SAM-1 showed a similar effect and SAM-3, had less effect than Loperamide. The findings suggest that probiotics and herbal residue could contribute to diarrhoeal disease prevention, which might be an alternative to a synthetic standard drug (Loperamide).

本研究旨在利用草药残渣的热水溶液提取物(HRE)来促进细菌生长,并具有止泻作用。本研究从枣椰树汁液(冬季采集)中分离出乳酸杆菌 L. brevis(SAM-1)、植物乳杆菌 Lactobacillus plantarum(SAM-2)和草本乳杆菌 Lactobacillus herbinensis(SAM-3)。孟加拉国 Square Pharmaceuticals PLC 公司提供的草药残留物 "Adovas "是一种非镇静型草药止咳糖浆,含有 16 种常见草药,其中包括 Adhatoda vasica。根据我们的观察,HRE 增加了 MRS 培养基中的菌落数量。在用蓖麻油和硫酸镁诱导的腹泻小鼠模型进行的抗腹泻研究中,含有或不含 HRE 的 SAM-1 和 2 显示出几乎相似的结果。在初步形态特征鉴定之后,又进行了耐低 pH 值、耐胆盐和在胃模拟液(GSF)中存活能力等测试,以确认它们是候选益生菌。这三种分离物均为革兰氏阳性菌,可在中嗜酸性温度范围内生长。分离菌的催化酶阴性,在过夜培养后能凝固牛奶。由于分离出的益生菌耐低 pH 值,并能耐受胆汁盐,因此可在胃肠中存活,是一种很有前途的候选益生菌。分离出的益生菌和 HRE 可抑制腹泻并恢复人体电解质。有趣的是,SAM-2 的功效高于标准药物(洛哌丁胺),而 SAM-1 的功效与之相似,SAM-3 的功效则低于洛哌丁胺。研究结果表明,益生菌和草药残留物有助于预防腹泻疾病,可作为合成标准药物(洛哌丁胺)的替代品。
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引用次数: 0
Investigating next-generation edible packaging: Protein-based films and coatings for delivering active compounds 研究下一代食用包装:用于输送活性化合物的蛋白质薄膜和涂层
IF 4.6 Q1 CHEMISTRY, APPLIED Pub Date : 2024-06-17 DOI: 10.1016/j.fhfh.2024.100182
Myat Noe Khin , Shabbir Ahammed , Md. Murtuza Kamal , Md Nazmus Saqib , Fei Liu , Fang Zhong

Edible film and coating are nutritious and beneficial for the host as those are consumed with food. Among various edible films and coatings, this review focused on protein-based films and coatings due to their potential application as a carrier for bioactive compounds in the food and biomedical industries. Bioactive compounds such as probiotics, prebiotics, and phenolic compounds have shown promise in maintaining intestinal health. They enhance immune response, lower inflammation in gastrointestinal illnesses, and help to prevent colon cancer. However, these bioactive compounds are often susceptible to environmental factors such as temperature, oxygen, pH etc. Consequently, encapsulation of these compounds becomes essential to protect them from potential damage and ensure the delivery of these compounds into the host body while retaining their intended functional properties. Current trends involve incorporating phenolic compounds into films or encapsulating probiotics and prebiotics as core materials using different wall materials. These encapsulated compounds can be intake with the food. Ongoing research endeavors are dedicated to improve the mechanical properties or functional properties of edible films and coatings separately. This review aims to overcome existing limitations of encapsulation of bioactive compounds into various types of protein film and enhance the functionality and health benefits and unlock the application of protein-based edible films and coating in the food industry.

可食用薄膜和涂层与食物一起食用,营养丰富,对宿主有益。在各种可食用薄膜和涂层中,本综述重点关注基于蛋白质的薄膜和涂层,因为它们有可能作为生物活性化合物的载体应用于食品和生物医学行业。益生菌、益生元和酚类化合物等生物活性化合物在维护肠道健康方面大有可为。它们能增强免疫反应,降低胃肠道疾病的炎症反应,并有助于预防结肠癌。然而,这些生物活性化合物往往容易受到温度、氧气、pH 值等环境因素的影响。因此,必须对这些化合物进行封装,以保护它们免受潜在的损害,并确保将这些化合物输送到宿主体内,同时保留其预期的功能特性。目前的趋势是将酚类化合物加入薄膜中,或使用不同的壁材将益生菌和益生元作为核心材料进行封装。这些封装化合物可与食物一起摄入。目前正在进行的研究致力于分别改善可食用薄膜和涂层的机械性能或功能特性。本综述旨在克服将生物活性化合物封装到各种类型的蛋白质薄膜中的现有局限性,增强其功能性和健康益处,并开启蛋白质基可食用薄膜和涂层在食品工业中的应用。
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引用次数: 0
Recent advances on antimicrobial peptide and polysaccharide hydrogels 抗菌肽和多糖水凝胶的最新进展
IF 4.6 Q1 CHEMISTRY, APPLIED Pub Date : 2024-06-13 DOI: 10.1016/j.fhfh.2024.100180
Serena Lam , Amanda Clairoux , Chibuike C. Udenigwe

The three-dimensional structure and network that compose naturally or synthetically derived polymers, such as hydrogels, allow for a wide variety in customization of the biomaterial characteristics, thus resulting in various applications. In medical care, hydrogels formed by intrinsic or exogenous antimicrobial components can act as effective vehicles for the administration of drugs and bioactive compounds, as alternatives to traditional wound dressings, and as antimicrobial coatings on implanted medical devices. In food safety, hydrogels with antimicrobial properties are desirable as food spoilage inhibitors. There has been a recent heightened focus on naturally derived hydrogels, due to their cost effectiveness and lack of concern for toxicity, which enhance their potential for a variety of food and biomedical applications. This concise review focuses on the recent advances of naturally derived peptide and polysaccharide antimicrobial hydrogels in the biomedical and food industries.

天然或合成聚合物(如水凝胶)的三维结构和网络使生物材料特性的定制变得多种多样,从而产生了各种应用。在医疗领域,由内在或外源性抗菌成分形成的水凝胶可作为给药和生物活性化合物的有效载体、传统伤口敷料的替代品以及植入式医疗设备的抗菌涂层。在食品安全方面,具有抗菌特性的水凝胶是理想的食品腐败抑制剂。近来,人们对天然提取的水凝胶的关注度越来越高,因为这些水凝胶具有成本效益,而且不存在毒性问题,从而提高了它们在各种食品和生物医学应用中的潜力。这篇简明综述重点介绍了天然提取的肽和多糖抗菌水凝胶在生物医学和食品行业的最新进展。
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引用次数: 0
Effect of gum extracts on the bread-making and textural properties of dough and bread made from sour cassava starch (Manihot esculenta), Peanut (Arachis hypogaea) and cowpea flour (Vigna unguiculata) 树胶提取物对用酸木薯淀粉(Manihot esculenta)、花生(Arachis hypogaea)和豇豆粉(Vigna unguiculata)制作的面团和面包的面包制作和质地特性的影响
IF 1.9 Pub Date : 2024-04-21 DOI: 10.1016/j.fhfh.2024.100179
Marie Madeleine Nanga Ndjang , Julie Mathilde Klang , Bilkissou Njapndounke , Marius Edith Kouam Foko , Jean Roger Dongmo , Michael Hermann Kengne Kamdem , Jordan Lembe Tonga , Edwin Mpho Mmutlane , Derek Tantoh Ndinteh , Eugenie Kayitesi , François Ngoufack Zambou

Gluten intolerance, as well as the scarcity of wheat flour in some parts of the world, has prompted the development of gluten-free bread. Gluten-free bread, on the other hand, results in a low specific volume and to remedy this, the use of hydrocolloids and bases has proved to be very successful. The current study aims to determine the optimal proportions of Triumffeta pentendra gum extract and bicarbonate in the breadmaking of a composite flour based on sour cassava starch, peanut flour, and cowpea flour. A Box Benkhen design was used to achieved this, with the variables being the amount of gum extract, the amount of bicarbonate, and the amount of water. The specific volume and texture properties were evaluated as responses. The specific volume was calculated using standard methods, and the textural properties were determined using a texture analyzer. It appears that the incorporation of gums extract, bicarbonate, and water significantly increased the specific volume. The incorporation rate of gum extract significantly increases the hardness, consistency, and masticability which decreases with the incorporation rate of bicarbonate and water. Cohesion and elasticity, on the other hand, increased with the incorporation rate of bicarbonate and water but decreased with the incorporation of gum extract. The optimal gum extract, bicarbonate, and water proportions are 0.28 g, 1.99 g, and 112.5 ml, respectively. As a result, the specific volume is 1.51cm3/g, the hardness is 38.51(N), the cohesion is 0.88, the consistency is 32.86(N), the elasticity is 5.57(1/L), and the masticability is 162.35(mj). According to this findings, gum extracts and sodium bicarbonate can be used to improve the quality of gluten-free bread made with sour cassava starch, peanut and cowpea flour.

麸质不耐症以及世界上某些地区小麦粉的稀缺促使了无麸质面包的发展。另一方面,无麸质面包的比容较低,为了解决这一问题,使用亲水胶体和基质被证明是非常成功的。本研究旨在确定 Triumffeta pentendra 胶提取物和碳酸氢盐在以酸木薯淀粉、花生粉和豇豆粉为基础的复合面粉面包制作中的最佳比例。为此采用了箱式本肯设计,变量为胶提取物的用量、碳酸氢盐的用量和水的用量。比容和质地特性作为反应进行评估。比容用标准方法计算,质地特性用质地分析仪测定。结果表明,加入树胶提取物、碳酸氢盐和水后,比容明显增加。树胶提取物的添加量可明显提高硬度、稠度和可咀嚼性,而碳酸氢盐和水的添加量则会降低硬度、稠度和可咀嚼性。另一方面,内聚力和弹性随碳酸氢盐和水的添加量增加而增加,但随胶提取物的添加量减少而减少。胶提取物、碳酸氢盐和水的最佳比例分别为 0.28 克、1.99 克和 112.5 毫升。结果,比容为 1.51cm3/g,硬度为 38.51(N),内聚力为 0.88,稠度为 32.86(N),弹性为 5.57(1/L),可咀嚼性为 162.35(mj)。根据这一研究结果,胶提取物和碳酸氢钠可用于改善用酸木薯淀粉、花生粉和豇豆粉制作的无筋面包的质量。
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引用次数: 0
Microencapsulation of curcumin by complex coacervation of lactoferrin and carboxymethyl tara gum for incorporation into edible films 通过乳铁蛋白和羧甲基塔拉胶的复合共凝胶对姜黄素进行微胶囊化,以便将其纳入可食用薄膜中
IF 1.9 Pub Date : 2024-01-24 DOI: 10.1016/j.fhfh.2024.100178
Barbara da Silva Soares , Augusto Bene Tomé Constantino , Edwin Elard Garcia-Rojas

Curcumin is a natural bioactive agent found in turmeric (Curcuma longa) with many health benefits, but with susceptibility to alkaline conditions, light, oxidation and heat. The present research aimed microencapsulate curcumin by complex coacervation using lactoferrin (LF) and carboxymethyl tara gum (CMTG) for application in edible films. The study of wall material formation was carried out by phase diagram, zeta potential and isothermal titration calorimetry. Curcumin was encapsulated by complex coacervation using different core-to-wall ratios and total biopolymer concentration. Finally, the microcapsules were used for the fabrication of edible gelatin-based films. The results showed that LF and CMTG could form complexes at pH 4.5 by electrostatic attraction with high affinity and optimum encapsulation efficiency of curcumin (74.78 %). The microcapsules protected curcumin during the oral and gastric phases with an average release in the intestinal phase of 81.81 %. After in vitro gastrointestinal digestion, the bioaccessibility of encapsulated curcumin was approximately 67 %. Curcumin microcapsules were added to edible gelatin films, which resulted in reduced light transmission and presence of antioxidant activity (FRAP and DPPH·+). The films containing microcapsules had their mechanical properties preserved. Therefore, curcumin-containing microcapsules formed by complex coacervation of LF/CMTG can be used in the production of edible films with high functional properties.

姜黄素是姜黄(Curcuma longa)中的一种天然生物活性物质,对健康有诸多益处,但易受碱性条件、光、氧化和热的影响。本研究旨在利用乳铁蛋白(LF)和羧甲基塔拉胶(CMTG)的复合共凝胶对姜黄素进行微胶囊化,以应用于可食用薄膜。通过相图、ZETA电位和等温滴定量热法研究了壁材的形成。使用不同的芯壁比和生物聚合物总浓度,通过复合共凝胶法封装姜黄素。最后,微胶囊被用于制作可食用明胶基薄膜。结果表明,LF 和 CMTG 可在 pH 值为 4.5 的条件下通过静电吸引形成复合物,具有很高的亲和力和最佳的姜黄素封装效率(74.78%)。微胶囊能在口腔和胃部阶段保护姜黄素,在肠道阶段的平均释放率为 81.81%。经过体外胃肠道消化后,封装姜黄素的生物可及性约为 67%。将姜黄素微胶囊添加到可食用明胶薄膜中,可降低透光率并提高抗氧化活性(FRAP 和 DPPH-+)。含有微胶囊的薄膜保留了其机械性能。因此,通过 LF/CMTG 复合共凝胶形成的含姜黄素微胶囊可用于生产具有高功能特性的可食用薄膜。
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引用次数: 0
Formation of alginate/ Alyssum homolocarpum seed gum (AHSG) microgels through electrospraying technique to encapsulate and release curcumin 通过电喷雾技术形成海藻酸/杓兰种子胶(AHSG)微凝胶,以封装和释放姜黄素
IF 1.9 Pub Date : 2024-01-23 DOI: 10.1016/j.fhfh.2024.100177
Seyedeh Fatemeh Mousavi , Arash Koocheki , Behrouz Ghorani , Mohebbat Mohebbi

This study aimed to produce microgels by electrospraying of alginate/AHSG (Alyssum homolocarpum seed gum) dispersions at different mixing ratios (1:0, 3:1, 1:1) for encapsulation of curcumin. Addition of AHSG to alginate increased the ζ-potential while it reduced the viscosity of the dispersions. Increased alginate to AHSG ratio resulted in smaller microgels and more uniform morphology. Results confirmed that curcumin was physically entrapped within the microgels matrix. As the alginate to AHSG ratio decreased, the microgel's encapsulation efficiency and thermal stability (∆H) increased from 87.76 to 95.85 % and 23.84 to 40.33 (J/g), respectivly. The lowest curcumin release rate during storage was for 3:1 alginate:AHSG microgel (41.4 %). The microgels provided more protection for curcumin against UV irradiation. The release profiles of curcumin from microgels during in vitro digestion were controlled by the Fickian diffusion phenomenon. Overall, these results indicated that electrosprayed alginate/AHSG microgels enhanced the photostability and improved the controlled release of curcumin throughout the gastrointestinal tract.

本研究旨在通过电喷雾法生产不同混合比例(1:0、3:1、1:1)的海藻酸盐/AHSG(Alyssum homolocarpum seed gum)分散体微凝胶,以封装姜黄素。在海藻酸盐中添加 AHSG 增加了ζ电位,同时降低了分散体的粘度。提高海藻酸与 AHSG 的比例可使微凝胶更小,形态更均匀。结果证实,姜黄素被物理性地包裹在微凝胶基质中。随着海藻酸盐与 AHSG 比率的降低,微凝胶的封装效率和热稳定性(ΔH)分别从 87.76% 和 23.84% 提高到 95.85% 和 40.33 (J/g)。储存期间姜黄素释放率最低的是 3:1 的海藻酸:AHSG 微凝胶(41.4%)。微凝胶能更好地保护姜黄素免受紫外线照射。在体外消化过程中,姜黄素从微凝胶中的释放曲线受菲氏扩散现象控制。总之,这些结果表明,电喷雾海藻酸盐/AHSG 微凝胶增强了姜黄素的光稳定性,并改善了姜黄素在整个胃肠道的控释。
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引用次数: 0
Ultrasound-assisted extraction of anthocyanin from black rice bran and its encapsulation by complex coacervation 超声波辅助提取黑米糠中的花青素并通过复合共凝胶对其进行封装
IF 1.9 Pub Date : 2024-01-10 DOI: 10.1016/j.fhfh.2023.100174
Lourembam Monika Devi, Amit Baran Das, Laxmikant S. Badwaik

The present work was planned to optimize extraction process of phenolics, flavonoids, monomeric anthocyanin and antioxidants from black rice bran using Ultrasound-assisted extraction and finally utilize it for encapsulation using double emulsion coacervation process. Response surface methodology (RSM) was employed for optimization of extraction process. Acidified ethanol (1 M HCl) was used as extraction solvent and ultrasound power (W) and extraction time (min) were used as independent variables. Face centered composite design (FCCD) data was successfully opted and developed a 2nd order polynomial equation with R2 value of 0.94 for total phenolic content (TPC), 0.95 for total flavonoid content (TFC), 0.87 for 1,1-diphenyl-2-picrylhydrazyl (DPPH) and 0.98 for total anthocyanin content (ANCs) respectively. Kinetic model study for extraction of anthocyanin was compared to conventional extraction process. Characterization of the extracts was performed using high pressure liquid chromatography analysis (HPLC). Cyanidin-3-glucoside is the predominated anthocyanin found as compared to peonidin-3-D-glucoside. Finally, coacervated microcapsules were developed using anthocyanin extract as core solution and gelatin and acacia gum as wall materials. Characterization of the microcapsules in terms of moisture content, hygroscopicity, solubility, encapsulation efficiency and surface morphology were evaluated. The result thus obtained shows that potential anthocyanin content from black rice bran can be utilized as food ingredients for various food application and formulation of functional food.

本研究计划利用超声波辅助萃取法优化黑米糠中酚类、类黄酮、单体花青素和抗氧化剂的萃取过程,最后利用双乳液共凝胶工艺将其封装。采用响应面法(RSM)对提取工艺进行了优化。酸化乙醇(1 M HCl)用作提取溶剂,超声功率(瓦)和提取时间(分钟)用作自变量。成功地选择了面心复合设计(FCCD)数据,并建立了二阶多项式方程,总酚含量(TPC)的 R2 值为 0.94,总黄酮含量(TFC)的 R2 值为 0.95,1,1-二苯基-2-苦基肼(DPPH)的 R2 值为 0.87,总花青素含量(ANCs)的 R2 值为 0.98。花青素提取动力学模型研究与传统提取工艺进行了比较。使用高压液相色谱法(HPLC)对提取物进行了表征。与芍药苷-3-D-葡萄糖苷相比,花青素-3-葡萄糖苷是主要的花青素。最后,以花青素提取物为核心溶液,明胶和刺槐胶为壁材,开发了共凝微胶囊。从含水量、吸湿性、溶解度、封装效率和表面形态等方面对微胶囊的特性进行了评估。结果表明,黑米糠中潜在的花青素成分可作为食品配料用于各种食品应用和功能食品配方。
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引用次数: 0
Effect of Vicia villosa protein isolate-based edible coating incorporated with ZnO nanoparticles on the shelf-life of chicken breast meat during cold storage 以紫云英蛋白分离物为基础并加入氧化锌纳米颗粒的可食用涂层对鸡胸肉冷藏货架期的影响
IF 1.9 Pub Date : 2024-01-09 DOI: 10.1016/j.fhfh.2024.100176
Ashkan Jebeli Javan , Sahar Baktash , Behdad Shokrolahi Yancheshmeh , Mahnoosh Parsaeimehr , Hamid Madanchi , Anna Abdolshahi , Leila Monjazeb Marvdashti , Nabi Shriatifar

In this study, Vicia villosa protein isolate (VVPI), with a high and valuable protein source, was used to create an edible coating containing ZnO nanoparticles (ZnO NPs) to investigate the effect of active coating on the quality of chicken breast fillets during refrigerated storage. The results showed a low growth rate in total viable count (TVC) and lactic acid bacteria (LAB) in coated samples. Moreover, as ZnO NPs concentration increased, thiobarbituric acid reactive substances (TBARS) decreased in the coated samples. In addition, the acid value and total volatile basic nitrogen (TVB-N) in the coated samples were significantly lower than the control group. Fourier transform infrared (FTIR) spectrum confirmed the chemical interactions of the coating components. Scanning electron microscopy (SEM) showed a homogeneous layer of coating on the surface of the coated chicken meat. Organoleptic indicators including color, smell, texture and overall acceptability were acceptable in all coated samples. These findings showed that the coating based on protein isolate containing ZnO NPs has a good potential to increase the safety and shelf life of chicken meat.

在这项研究中,使用了具有高价值蛋白质来源的紫云英蛋白分离物(VVPI)来制作含有氧化锌纳米粒子(ZnO NPs)的可食用涂层,以研究活性涂层对冷藏贮藏期间鸡胸肉片质量的影响。结果表明,涂层样品中的总存活数(TVC)和乳酸菌(LAB)增长率较低。此外,随着 ZnO NPs 浓度的增加,涂层样品中硫代巴比妥酸活性物质(TBARS)的含量也有所下降。此外,包衣样品中的酸值和总挥发性碱基氮(TVB-N)也明显低于对照组。傅立叶变换红外光谱(FTIR)证实了涂层成分之间的化学作用。扫描电子显微镜(SEM)显示,涂层鸡肉表面有一层均匀的涂层。所有涂层样品的色泽、气味、质地和总体可接受性等感官指标均可接受。这些研究结果表明,基于含有氧化锌纳米粒子的分离蛋白的涂层具有提高鸡肉安全性和货架期的良好潜力。
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引用次数: 0
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Food Hydrocolloids for Health
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