Electroacupuncture relieves postoperative cognitive dysfunction in elderly rats via regulating amp-activated protein kinase autophagy signaling

IF 16.4 1区化学 Q1 CHEMISTRY, MULTIDISCIPLINARY Accounts of Chemical Research Pub Date : 2022-03-01 DOI:10.4103/cjp.cjp_108_21

Cong-cong Niu, M. Zhu, Jiamin Zhang, Chenye Zhang, Weiqian Tian

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Abstract

Postoperative cognitive dysfunction (POCD) is a common complication after surgery in elderly patients. Electroacupuncture (EA) has been reported to relieve POCD in animal models, but the mechanism remains fully elucidated. The objective of this work was to clarify whether EA could alleviate POCD via regulating autophagy. In this study, aged rats were assigned into 4 groups: control, surgery (rats underwent exploratory laparotomy to induce POCD), EA + S (rats received EA pre-stimulation before surgery), and EA + S + Chloroquine (CQ) (rats were intraperitoneally injected with CQ before EA stimulation and then underwent surgery). The cognitive function of rats was assessed by Morris Water Maze (MWM) test after surgery, and autophagy in hippocampal tissues of rats was evaluated by western blotting and transmission electron microscope. Results indicated that the MWM test revealed that rats showed reduced platform crossing and increased total swimming distance after surgery. However, this impaired spatial memory was improved by EA and EA plus CQ pre-treatment. Besides, the surgery caused an increased expression in LC3II, Beclin-1, AMP-activated protein kinase (AMPK), and p-AMPK in hippocampal tissues of rats, while EA and EA plus CQ pre-treatment also reversed this effect. In addition, the surgery-induced increased amount of autophagic vesicles in hippocampal tissues of rats was reduced by EA and EA plus CQ pre-treatment. In conclusion, EA pre-stimulation could effectively attenuate cognitive impairment in aged rats with POCD via inhibiting AMPK signaling-mediated autophagy.

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电针通过调节amp活化蛋白激酶自噬信号减轻老年大鼠术后认知功能障碍

术后认知功能障碍（POCD）是老年患者术后常见的并发症。电针（EA）已被报道可在动物模型中缓解POCD，但其机制尚不完全清楚。这项工作的目的是阐明电针是否可以通过调节自噬来减轻POCD。在本研究中，老年大鼠被分为4组：对照组、手术组（大鼠进行剖腹探查以诱导POCD）、EA+S组（大白鼠在手术前接受电针预刺激）和EA+S+氯喹（CQ）组（大老鼠在电针刺激前腹膜内注射CQ，然后进行手术）。术后用Morris Water-Maze（MWM）试验评价大鼠的认知功能，用western印迹和透射电镜评价大鼠海马组织的自噬。结果表明，MWM测试显示，大鼠在手术后平台交叉减少，总游泳距离增加。然而，电针和电针加CQ预处理改善了这种受损的空间记忆。此外，手术导致大鼠海马组织中LC3II、Beclin-1、AMP活化蛋白激酶（AMPK）和p-AMPK的表达增加，而电针和电针加CQ预处理也逆转了这种作用。此外，手术诱导的大鼠海马组织中自噬小泡数量的增加通过电针和电针加CQ预处理而减少。总之，电针预刺激可以通过抑制AMPK信号介导的自噬有效减轻老年POCD大鼠的认知障碍。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Accounts of Chemical Research 化学-化学综合

CiteScore

31.40

自引率

1.10%

发文量

312

审稿时长

2 months

期刊介绍： Accounts of Chemical Research presents short, concise and critical articles offering easy-to-read overviews of basic research and applications in all areas of chemistry and biochemistry. These short reviews focus on research from the author’s own laboratory and are designed to teach the reader about a research project. In addition, Accounts of Chemical Research publishes commentaries that give an informed opinion on a current research problem. Special Issues online are devoted to a single topic of unusual activity and significance. Accounts of Chemical Research replaces the traditional article abstract with an article "Conspectus." These entries synopsize the research affording the reader a closer look at the content and significance of an article. Through this provision of a more detailed description of the article contents, the Conspectus enhances the article's discoverability by search engines and the exposure for the research.