Expression Patterns of Heat Shock Protein Genes and Antioxidase Genes in Apis cerana cerana (Hymenoptera: Apidae) under Heat Stress

IF 0.7 4区 农林科学 Q4 ENTOMOLOGY Journal of Entomological Science Pub Date : 2023-01-24 DOI:10.18474/JES22-27
Xinyu Li, Weihua Ma, Yusuo Jiang
{"title":"Expression Patterns of Heat Shock Protein Genes and Antioxidase Genes in Apis cerana cerana (Hymenoptera: Apidae) under Heat Stress","authors":"Xinyu Li, Weihua Ma, Yusuo Jiang","doi":"10.18474/JES22-27","DOIUrl":null,"url":null,"abstract":"Abstract Heat stress elicits the expression of various antistress proteins (e.g., heat shock proteins [HSPs] and antioxidase enzymes) in honeybees (Hymenoptera: Apidae), which are important in protecting cells from heat-induced stresses. In this study, we used real-time quantitative reverse transcription–polymerase chain reaction to analyze the expression patterns of the heat shock protein 90 (Hsp90), heat shock protein 70Ab (Hsp70Ab), peroxidase (Pod), and cytochrome P450 (Cyp450) in response of Apis cerana cerana F. to different temperatures and different heat exposure times. We observed that, with the increase of temperature and exposure time, the expression of the four genes also increased, thus confirming that heat stress can activate heat-resistant mechanisms of A. cerana cerana and that temperature and exposure time are key factors affecting the accumulation of HSPs. Our results provide information on the expression patterns of four genes during heat stress to serve as a basis for determining the mechanisms by which A. cerana cerana adapts to thermal stress.","PeriodicalId":15765,"journal":{"name":"Journal of Entomological Science","volume":"58 1","pages":"95 - 103"},"PeriodicalIF":0.7000,"publicationDate":"2023-01-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"1","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Journal of Entomological Science","FirstCategoryId":"97","ListUrlMain":"https://doi.org/10.18474/JES22-27","RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q4","JCRName":"ENTOMOLOGY","Score":null,"Total":0}
引用次数: 1

Abstract

Abstract Heat stress elicits the expression of various antistress proteins (e.g., heat shock proteins [HSPs] and antioxidase enzymes) in honeybees (Hymenoptera: Apidae), which are important in protecting cells from heat-induced stresses. In this study, we used real-time quantitative reverse transcription–polymerase chain reaction to analyze the expression patterns of the heat shock protein 90 (Hsp90), heat shock protein 70Ab (Hsp70Ab), peroxidase (Pod), and cytochrome P450 (Cyp450) in response of Apis cerana cerana F. to different temperatures and different heat exposure times. We observed that, with the increase of temperature and exposure time, the expression of the four genes also increased, thus confirming that heat stress can activate heat-resistant mechanisms of A. cerana cerana and that temperature and exposure time are key factors affecting the accumulation of HSPs. Our results provide information on the expression patterns of four genes during heat stress to serve as a basis for determining the mechanisms by which A. cerana cerana adapts to thermal stress.
查看原文
分享 分享
微信好友 朋友圈 QQ好友 复制链接
本刊更多论文
热应激条件下中华蜜蜂热休克蛋白基因和抗氧化酶基因的表达模式
热应激引起蜜蜂(膜翅目:蜂科)多种抗应激蛋白(如热休克蛋白[HSPs]和抗氧化酶)的表达,这些蛋白在保护细胞免受热应激的影响中起重要作用。本研究采用实时定量逆转录-聚合酶链反应技术,分析了中国蜜蜂热休克蛋白90 (Hsp90)、热休克蛋白70Ab (Hsp70Ab)、过氧化物酶(Pod)和细胞色素P450 (Cyp450)在不同温度和不同热暴露时间下的表达规律。我们观察到,随着温度和暴露时间的增加,这四个基因的表达也增加,从而证实了热胁迫可以激活中华蜜蜂的耐热机制,温度和暴露时间是影响热休克蛋白积累的关键因素。我们的结果提供了四个基因在热应激过程中的表达模式信息,为确定中华蜜蜂适应热应激的机制提供了基础。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
求助全文
约1分钟内获得全文 去求助
来源期刊
CiteScore
1.20
自引率
11.10%
发文量
40
审稿时长
>12 weeks
期刊介绍: The Journal of Entomological Science (ISSN 0749-8004) is a peer-reviewed, scholarly journal that is published quarterly (January, April, July, and October) under the auspices of the Georgia Entomological Society in concert with Allen Press (Lawrence, Kansas). Manuscripts deemed acceptable for publication in the Journal report original research with insects and related arthropods or literature reviews offering foundations to innovative directions in entomological research
期刊最新文献
Impact of Pecan Leafroll Mite (Acari: Eriophyidae) on Pecan Foliage1 Susceptibility of Bemisia tabaci (Hemiptera: Aleyrodidae) Adult Populations to Imidacloprid in Georgia, USA1 Host Plant Feeding and Ovipositional Preferences of Spodoptera frugiperda (Lepidoptera: Noctuidae) under Laboratory Conditions1 Field Evaluation of Biological and Conventional Insecticides for Managing Multiple Insect Pests in Cotton1 DNA Barcoding of Locusta migratoria manilensis (Orthoptera: Acrididae) Reveals Insights into the Species and Subspecies Differentiation1
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
现在去查看 取消
×
提示
确定
0
微信
客服QQ
Book学术公众号 扫码关注我们
反馈
×
意见反馈
请填写您的意见或建议
请填写您的手机或邮箱
已复制链接
已复制链接
快去分享给好友吧!
我知道了
×
扫码分享
扫码分享
Book学术官方微信
Book学术文献互助
Book学术文献互助群
群 号:481959085
Book学术
文献互助 智能选刊 最新文献 互助须知 联系我们:info@booksci.cn
Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。
Copyright © 2023 Book学术 All rights reserved.
ghs 京公网安备 11010802042870号 京ICP备2023020795号-1