{"title":"Expression Patterns of Heat Shock Protein Genes and Antioxidase Genes in Apis cerana cerana (Hymenoptera: Apidae) under Heat Stress","authors":"Xinyu Li, Weihua Ma, Yusuo Jiang","doi":"10.18474/JES22-27","DOIUrl":null,"url":null,"abstract":"Abstract Heat stress elicits the expression of various antistress proteins (e.g., heat shock proteins [HSPs] and antioxidase enzymes) in honeybees (Hymenoptera: Apidae), which are important in protecting cells from heat-induced stresses. In this study, we used real-time quantitative reverse transcription–polymerase chain reaction to analyze the expression patterns of the heat shock protein 90 (Hsp90), heat shock protein 70Ab (Hsp70Ab), peroxidase (Pod), and cytochrome P450 (Cyp450) in response of Apis cerana cerana F. to different temperatures and different heat exposure times. We observed that, with the increase of temperature and exposure time, the expression of the four genes also increased, thus confirming that heat stress can activate heat-resistant mechanisms of A. cerana cerana and that temperature and exposure time are key factors affecting the accumulation of HSPs. Our results provide information on the expression patterns of four genes during heat stress to serve as a basis for determining the mechanisms by which A. cerana cerana adapts to thermal stress.","PeriodicalId":15765,"journal":{"name":"Journal of Entomological Science","volume":"58 1","pages":"95 - 103"},"PeriodicalIF":0.7000,"publicationDate":"2023-01-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"1","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Journal of Entomological Science","FirstCategoryId":"97","ListUrlMain":"https://doi.org/10.18474/JES22-27","RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q4","JCRName":"ENTOMOLOGY","Score":null,"Total":0}
引用次数: 1
Abstract
Abstract Heat stress elicits the expression of various antistress proteins (e.g., heat shock proteins [HSPs] and antioxidase enzymes) in honeybees (Hymenoptera: Apidae), which are important in protecting cells from heat-induced stresses. In this study, we used real-time quantitative reverse transcription–polymerase chain reaction to analyze the expression patterns of the heat shock protein 90 (Hsp90), heat shock protein 70Ab (Hsp70Ab), peroxidase (Pod), and cytochrome P450 (Cyp450) in response of Apis cerana cerana F. to different temperatures and different heat exposure times. We observed that, with the increase of temperature and exposure time, the expression of the four genes also increased, thus confirming that heat stress can activate heat-resistant mechanisms of A. cerana cerana and that temperature and exposure time are key factors affecting the accumulation of HSPs. Our results provide information on the expression patterns of four genes during heat stress to serve as a basis for determining the mechanisms by which A. cerana cerana adapts to thermal stress.
期刊介绍:
The Journal of Entomological Science (ISSN 0749-8004) is a peer-reviewed, scholarly journal that is published quarterly (January, April, July, and October) under the auspices of the Georgia Entomological Society in concert with Allen Press (Lawrence, Kansas). Manuscripts deemed acceptable for publication in the Journal report original research with insects and related arthropods or literature reviews offering foundations to innovative directions in entomological research