RAPID SCREENING OF ANTIBACTERIAL AND ANTIOXIDANT METABOLITES FROM ENDOPHYTIC FUNGI ISOLATED FROM Papuacedrus papuana BY TLC-BIOAUTOGRAPHY

Abstract

RAPID SCREENING OF ANTIBACTERIAL AND ANTIOXIDANT METABOLITES FROM ENDOPHYTIC FUNGI ISOLATED FROM Papuacedrus papuana. Papuacedrus papuana is a plant species that grows in the highland of Papua. The aims of the study were to determine in vitro antibacterial activity and antioxidant activity of 17 endophytic fungal extracts isolated from P. papuana stem by the TLC-bioautography method. The antibacterial activity was assessed against Staphylococcus aureus InaCC B4 and Escherichia coli InaCC B5. The antioxidant activity was assessed by DPPH radical scavenging assay. All of 17 endophytic fungi were grouped into 7 taxa based on their morphological traits. The results showed that 11 fungal extracts were active against S. aureus InaCC B4, 15 fungal extracts were active against E. coli InaCC B5, and 6 extracts had antioxidant activity. Further analysis of active extracts by eluted TLC-bioautography showed several compounds responsible for antibacterial or antioxidant activity in one extract. The results showed a diversity of endophytic fungi inhabited P. papuana stem, and these endophytic fungi might be used as a good source of novel antibacterial or antioxidants.