Valorization of Catla Visceral Waste by Obtaining Industrially Important Enzyme: Trypsin

Abstract

The fish visceral waste is currently posing a major problem to fisheries and environment sustainability. India has generated fish waste around 50% of the production in the year 2015-16. The indiscriminate practices of waste disposal cause physical and ecological imbalances in the ecosystem. Hence, its management has become very important. The visceral organs are known sources of biologically active compounds therefore, value-added products can be recovered. The present study focused on isolation of visceral protease of popular fish species, Catla catla. The proteins were precipitated using various precipitating agents among which cold acetone was found best (152.93mg ml-1 protein). The kinetic study of the enzyme was performed using BAPNA substrate and enzyme activity (15.54 U ml-1), Km (2.47mM) and Vmax (0.40µm-1 min-1) were calculated. The molecular weight of the protease was 24KDa, optimum pH was 8.5, and optimum temperature was 40ºC. The enzyme was found 19.3% similar with mammalian (bovine) trypsin. Henceforth, the enzyme was thought to solve the fish visceral waste problem with an ecological approach.