Effect of BAP (6-Benzylaminopurine) and NAA (α-Napthalene Acetic Acid) treatment on Micropropagation of Adansonia digitata L.

Q4 Agricultural and Biological Sciences Plant Cell Biotechnology and Molecular Biology Pub Date : 2023-07-04 DOI:10.56557/pcbmb/2023/v24i3-48292
M. Rehman, M. Chaudhary, Sandeep Kumar
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Abstract

The African Baobab, also known as Kalpvriksha (Adansonia digitata L.), is a significant multi-purpose tree that has gained popularity among people, primarily in African countries, for its use as food, fodder, clothing, and ropes. There is one of the obstacle arises in growing this plant by farmers in India is the lack of planting materials. The young plants of A. digitata L. have to be imported form selected nurseries. Therefore, micropropagation can be used as a substitute method to create offspring that are genetically similar to the parent plant. In order to micropropagate Kalpvriksh (A. digitata L.), this experiment is being conducted utilizing shoot tip explants. For shoot proliferation, these explants are grown in MS medium containing various concentrations of BAP (6- Benzylamino purine): 0.5, 1.0, 1.5, 2.5, 5.0 mg/L. Shoots are rooted in a medium containing at the same concentration of NAA (α-Napthalene Acetic Acid) 0.5 mg/L alone or with combination. Complete Randomized Design (CRD) is used to conduct each experiment. ANOVA and the Duncan New Multiple Range test are used to examine the data. According to the technique for micropropagation of A. digitata L. The concentration of BAP and NAA was found to be 1.5 mg/L and 0.5 mg/L respectively which showed better enhancement for shoot regeneration and rooting for micropropagation of Kalpvriksha (Adansonia digitata L.) tree.    
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BAP(6-苄基氨基嘌呤)和NAA(α-萘乙酸)处理对洋地黄微繁殖的影响。
非洲猴面包树,也称为Kalpvriksha(Adansonia digitata L.),是一种重要的多用途树木,因其用作食物、饲料、衣服和绳索而在人们中广受欢迎,主要在非洲国家。印度农民种植这种植物的障碍之一是缺乏种植材料。洋地黄的幼苗必须从选定的苗圃进口。因此,微繁殖可以作为一种替代方法来创造与亲本植物基因相似的后代。本试验是利用茎尖外植体对洋地黄进行微繁殖。对于芽增殖,这些外植体在含有不同浓度BAP(6-苄基氨基嘌呤)的MS培养基中生长:0.5、1.0、1.5、2.5、5.0mg/L。芽在含有相同浓度的NAA(α-萘乙酸)0.5 mg/L单独或组合的培养基中生根。每个实验采用完全随机设计(CRD)。ANOVA和Duncan New Multiple Range检验用于检验数据。根据洋地黄的微繁殖技术,发现BAP和NAA的浓度分别为1.5mg/L和0.5mg/L,对洋地黄的芽再生和微繁殖生根有较好的促进作用。
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来源期刊
Plant Cell Biotechnology and Molecular Biology
Plant Cell Biotechnology and Molecular Biology Agricultural and Biological Sciences-Horticulture
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