{"title":"Culturing Rat Whole Ovary for UV Filter Benzophenone-3 Treatment","authors":"Clarisa Guillermina Santamaría, Julián Elías Abud, Enrique Hugo Luque, Laura Kass, Horacio Adolfo Rodríguez","doi":"10.1002/cptx.89","DOIUrl":null,"url":null,"abstract":"<p>We describe a detailed protocol to establish a newborn rat whole ovary culture, which enables the study of direct effects (independent of hypothalamic-pituitary-gonadal axis) of endocrine disrupting chemicals (EDCs), such as benzophenone-3 (BP-3). This method is useful to understand changes in follicle formation, primordial to primary transition, and expression of regulatory molecules linked to these processes and also provides an alternative to animal models. © 2019 by John Wiley & Sons, Inc.</p><p><b>Basic Protocol 1</b>: Rat ovarian surgery</p><p><b>Basic Protocol 2</b>: Whole organ/ovarian culture</p><p><b>Basic Protocol 3</b>: RNA isolation and quantitative real-time PCR</p><p><b>Basic Protocol 4</b>: Histological processing and staining</p>","PeriodicalId":72743,"journal":{"name":"Current protocols in toxicology","volume":"82 1","pages":""},"PeriodicalIF":0.0000,"publicationDate":"2019-11-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1002/cptx.89","citationCount":"1","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Current protocols in toxicology","FirstCategoryId":"1085","ListUrlMain":"https://onlinelibrary.wiley.com/doi/10.1002/cptx.89","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
引用次数: 1
Abstract
We describe a detailed protocol to establish a newborn rat whole ovary culture, which enables the study of direct effects (independent of hypothalamic-pituitary-gonadal axis) of endocrine disrupting chemicals (EDCs), such as benzophenone-3 (BP-3). This method is useful to understand changes in follicle formation, primordial to primary transition, and expression of regulatory molecules linked to these processes and also provides an alternative to animal models. © 2019 by John Wiley & Sons, Inc.
Basic Protocol 1: Rat ovarian surgery
Basic Protocol 2: Whole organ/ovarian culture
Basic Protocol 3: RNA isolation and quantitative real-time PCR
Basic Protocol 4: Histological processing and staining
二苯甲酮-3紫外滤光处理大鼠全卵巢培养
我们描述了一种建立新生大鼠全卵巢培养的详细方案，该方案能够研究内分泌干扰化学物质(EDCs)的直接影响(独立于下丘脑-垂体-性腺轴)，如二苯甲酮-3 (BP-3)。这种方法有助于理解卵泡形成的变化，从原始到初级的转变，以及与这些过程相关的调节分子的表达，也为动物模型提供了一种替代方法。©2019 by John Wiley &基础方案1:大鼠卵巢手术基础方案2:全器官/卵巢培养基础方案3:RNA分离和定量实时pcr基础方案4:组织学处理和染色
本文章由计算机程序翻译，如有差异，请以英文原文为准。
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