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{"title":"A Hands-On Guide to Brewing and Analyzing Beer in the Laboratory","authors":"Florian A. Thesseling, Peter W. Bircham, Stijn Mertens, Karin Voordeckers, Kevin J. Verstrepen","doi":"10.1002/cpmc.91","DOIUrl":null,"url":null,"abstract":"<p>Beer would not exist without microbes. During fermentation, yeast cells convert cereal-derived sugars into ethanol and CO<sub>2</sub>. Yeast also produces a wide array of aroma compounds that influence beer taste and aroma. The complex interaction between all these aroma compounds results in each beer having its own distinctive palette. This article contains all protocols needed to brew beer in a standard lab environment and focuses on the use of yeast in beer brewing. More specifically, it provides protocols for yeast propagation, brewing calculations and, of course, beer brewing. At the end, we have also included protocols for analyses that can be performed on the resulting brew, with a focus on yeast-derived aroma compounds. © 2019 The Authors.</p>","PeriodicalId":39967,"journal":{"name":"Current Protocols in Microbiology","volume":null,"pages":null},"PeriodicalIF":0.0000,"publicationDate":"2019-09-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://sci-hub-pdf.com/10.1002/cpmc.91","citationCount":"13","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Current Protocols in Microbiology","FirstCategoryId":"1085","ListUrlMain":"https://onlinelibrary.wiley.com/doi/10.1002/cpmc.91","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"","JCRName":"","Score":null,"Total":0}
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Abstract
Beer would not exist without microbes. During fermentation, yeast cells convert cereal-derived sugars into ethanol and CO2 . Yeast also produces a wide array of aroma compounds that influence beer taste and aroma. The complex interaction between all these aroma compounds results in each beer having its own distinctive palette. This article contains all protocols needed to brew beer in a standard lab environment and focuses on the use of yeast in beer brewing. More specifically, it provides protocols for yeast propagation, brewing calculations and, of course, beer brewing. At the end, we have also included protocols for analyses that can be performed on the resulting brew, with a focus on yeast-derived aroma compounds. © 2019 The Authors.
在实验室酿造和分析啤酒的实践指南
如果没有微生物,啤酒就不会存在。在发酵过程中,酵母细胞将谷物衍生的糖转化为乙醇和二氧化碳。酵母还会产生一系列影响啤酒味道和香气的香气化合物。所有这些香气化合物之间复杂的相互作用导致每种啤酒都有自己独特的调色板。本文包含在标准实验室环境中酿造啤酒所需的所有协议,并重点介绍啤酒酿造中酵母的使用。更具体地说,它提供了酵母繁殖、酿造计算,当然还有啤酒酿造的协议。最后,我们还包括了可以对最终酿造进行分析的协议,重点是酵母衍生的香气化合物。©2019作者。
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