Semen Cryopreservation Quality and Sperm Kinematics of Saanen Goats Using Different Diluents

Abstract

The success of artificial insemination (AI) in small ruminants, especially goats, depends on the quality of frozen semen. Therefore, the current study aimed to determine the quality of various diluents, including tris-egg yolk, AndroMed®, and OviXcell®, on semen quality. The fresh semen samples from three male Saanen goats aged 1.5-2 years were collected and the mean individual motility of samples was recorded at 70%. The cryopreservation quality of the semen was evaluated based on motility, viability, abnormality, and total sperm motility (TSM) indexes. The present laboratory experiment was performed with 3 treatments and 10 repetitions. The treatments in this study were T0 (tris-egg yolk), T1 (AndroMed®), and T2 (OviXcell®). The results showed no significant difference in the parameters of motility, viability, abnormality, and TSM among the treatment group. The kinematic parameters’ average path length, velocity curved linear, and linearity showed a significant difference in all treatment groups. However, there were no significant differences among the three groups in terms of motility, progressiveness, distance curved line, distance straight line, average velocity path, velocity straight line, straightness, amplitude lateral head, beat cross frequency, and wobble kinematic parameters. Motility was higher in T2 than in T0 and T1, viability was higher in T1 than in T0 and T2, and abnormality was lower in T1 than in T0 and T2. In conclusion, the use of various diluents, such as tris-egg yolk, AndroMed®, and OviXcell®, can maintain the quality of frozen spermatozoa for over 24 hours, including motility, viability, abnormality, and TSM. Kinematic parameters obtained using CASA IVOS II can provide relevant information for various parameters using these diluents.