Modified screen-printed electrochemical biosensor design compatible with mobile phones for detection of miR-141 used to pancreatic cancer biomarker

IF 5.5 3区材料科学 Q2 CHEMISTRY, MULTIDISCIPLINARY Carbon Letters Pub Date : 2023-07-06 DOI:10.1007/s42823-023-00545-9

Muhammed Bekmezci, Ramazan Bayat, Merve Akin, Zeynep Kazel Coguplugil, Fatih Sen

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引用次数: 2

Abstract

MicroRNAs (miRNAs) are emerging materials as ideal biomarkers for noninvasive cancer detection in the early phase. In this article, a simple and label-free electrochemical miRNA biosensor was developed. A single-stranded DNA (ss-DNA) probes were successfully mapped to f-MWCNT and hybridized with the target miR-141 sequence. The optimum peak points of the obtained hybridization were determined using Cyclic Voltammetry (CV) and Differential Pulse Voltammetry (DPV) methods. Significant peaks were observed in the results, depending on miR-141 at different concentrations. The linear relationship (ν) between redox peak currents (Ip) and scanning rate indicated that electron transfer (ET) between miR-141 and the electrode surface was accomplished successfully. In DPV measurements, miR-141 was measured with a low detection limit (LOD) in the 1.3–12 nM concentration range, and the LOD and limit of quantification (LOQ) results were found to be 3 and 9.1 pM, respectively. Besides, selectivity test was investigated for the biosensor using different target analytes and a significant difference in value was observed between the peak currents of miR-141, and other target molecules. This developed strategy has been found to detect miR-141 sensitively, selectively and without tags, and its integration into mobile devices has been successfully carried out.

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用于检测胰腺癌生物标志物miR-141的可兼容手机的改进丝网印刷电化学生物传感器设计

MicroRNAs (miRNAs)是一种新兴的材料，是早期无创癌症检测的理想生物标志物。本文研制了一种简单、无标记的电化学miRNA生物传感器。单链DNA (ss-DNA)探针成功地映射到f-MWCNT上，并与靶miR-141序列杂交。利用循环伏安法(CV)和差分脉冲伏安法(DPV)确定杂交的最佳峰点。根据不同浓度的miR-141，在结果中观察到显著的峰。氧化还原峰值电流(Ip)与扫描速率之间的线性关系(ν)表明miR-141与电极表面之间的电子转移(ET)成功完成。在DPV测量中，miR-141在1.3-12 nM浓度范围内以低检测限(LOD)测量，结果发现LOD和定量限(LOQ)分别为3 pM和9.1 pM。此外，使用不同的目标分析物对生物传感器进行了选择性测试，发现miR-141的峰值电流与其他目标分子之间存在显著差异。已经发现这种开发的策略可以灵敏地、选择性地和无标签地检测miR-141，并且已成功地将其集成到移动设备中。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Carbon Letters CHEMISTRY, MULTIDISCIPLINARY-MATERIALS SCIENCE, MULTIDISCIPLINARY

CiteScore

7.30

自引率

20.00%

发文量

118

期刊介绍： Carbon Letters aims to be a comprehensive journal with complete coverage of carbon materials and carbon-rich molecules. These materials range from, but are not limited to, diamond and graphite through chars, semicokes, mesophase substances, carbon fibers, carbon nanotubes, graphenes, carbon blacks, activated carbons, pyrolytic carbons, glass-like carbons, etc. Papers on the secondary production of new carbon and composite materials from the above mentioned various carbons are within the scope of the journal. Papers on organic substances, including coals, will be considered only if the research has close relation to the resulting carbon materials. Carbon Letters also seeks to keep abreast of new developments in their specialist fields and to unite in finding alternative energy solutions to current issues such as the greenhouse effect and the depletion of the ozone layer. The renewable energy basics, energy storage and conversion, solar energy, wind energy, water energy, nuclear energy, biomass energy, hydrogen production technology, and other clean energy technologies are also within the scope of the journal. Carbon Letters invites original reports of fundamental research in all branches of the theory and practice of carbon science and technology.