Determination of monosaccharide composition of kefiran using HPLC

IF 1.1 4区农林科学 Q3 AGRICULTURE, DAIRY & ANIMAL SCIENCE Mljekarstvo Pub Date : 2020-12-14 DOI:10.15567/mljekarstvo.2021.0105

Ç. Koçak

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引用次数: 1

Abstract

Kefiran is an exopolysaccharide (EPS) produced by specific microorganisms in the kefir grain. In this research, kefir grain was activated in various carbon sources (glucose, galactose or lactose) containing medium. The monosaccharides composition of the formed kefiran were determined by a high performance liquid chromatography (HPLC). HPLC with refractive index (RI) detector was used in order to make qualitative and quantitative analysis of sucrose, glucose, galactose, arabinose, xylose, and ribose present in kefiran. Also, the physical properties of differently kefiran formed were determined. The amount of kefiran in the control group and in the sample from the galactose containing medium were determined to be 511.00 and 610.55 mg/L, respectively. The kefiran form obtained from the medium containing galactose was more positively affected than in other media. The apparent viscosity, at a shear rate of 66.0 s-1of the kefiran produced in glucose containing medium, was determined to be 29.2 mPas. The surface morphologies of different kefirans were analysed and roughness parameters were determined by atomic force microscopy (AFM); the maximum height of lumps ranged from 16.80 to 22.10 nm. In this study, of sucrose, glucose, galactose, arabinose, xylose, and ribose were found in sugar composition in all kefiran samples.

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高效液相色谱法测定克非兰单糖组成

开非尔蛋白是由开非尔颗粒中的特定微生物产生的一种胞外多糖(EPS)。本研究将开菲尔颗粒在不同碳源(葡萄糖、半乳糖或乳糖)培养基中进行活化。用高效液相色谱法测定了所制得的克非兰的单糖组成。采用高效液相色谱-折射率检测器对克非兰中蔗糖、葡萄糖、半乳糖、阿拉伯糖、木糖和核糖进行定性和定量分析。测定了不同形态的克非兰的物理性质。对照组和含半乳糖培养基样品中克非兰的含量分别为511.00和610.55 mg/L。从含有半乳糖的培养基中获得的克非兰形式比在其他培养基中受到更积极的影响。在含葡萄糖培养基中生产的kefiran，在剪切速率为66.0 s-1时，表观粘度为29.2 mpa。利用原子力显微镜(AFM)分析了不同kefirans的表面形貌，测定了粗糙度参数;肿块的最大高度为16.80 ~ 22.10 nm。在本研究中，在所有克非兰样品的糖组成中发现了蔗糖、葡萄糖、半乳糖、阿拉伯糖、木糖和核糖。

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来源期刊

Mljekarstvo Agricultural and Biological Sciences-Animal Science and Zoology

CiteScore

1.90

自引率

41.70%

发文量

审稿时长

12 weeks

期刊介绍： Mljekarstvo is an open access, peer-reviewed international quarterly scientific journal. The first issue was published in 1951, by the Croatian Dairy Operators'' Association (today: Croatian Dairy Union, publisher). In a paper at a Union conference held 28 October 1951 in Zagreb it was said: "Our desire is that this magazine does not meet the fate of its predecessors, but that it continues to reflect the creative efforts and to provide guidelines for the producers as well as all other operators employed in the dairy industry." It is our pleasure today to say that wishes of the enthusiasts who attended the conference have come true, and the magazine Mljekarstvo during the last six decades was a reflection of the creative efforts of numerous dairy scientists and experts, and through its texts it served as a guideline in improving production and processing of milk and dairy products. Mljekarstvo has been following all the achievements of the dairy profession in Croatia, and it also gives the short surveys of world achievements. The result of the research of local and foreign scientists and experts always find their place in the magazine Mljekarstvo. It has been edited by our outstanding dairy experts employed at colleges, research institutions and dairy companies.