Pub Date : 2024-06-11DOI: 10.15567/mljekarstvo.2024.0301
B. Mioč
Around 10.5 million tonnes of sheep milk are produced worldwide, and around 3.1 million tonnes in Europe. In Europe, sheep milk production has stagnated slightly in recent years and currently accounts for around 1/3 of the global production. Due to its high content of dry matter, milk fat, total protein and casein, sheep milk is an excellent raw material for cheese production. Milk processing and cheese making are not only economically important, but also part of the cultural heritage based on a long tradition, often taking place in rural and less developed areas. Milk production and quality are very complex and are influenced by numerous factors, the most important of which are breed, feeding and body condition, parity and stage of lactation, milking and udder health, and environmental factors. In addition, different types of stress such as heat stress, nutritional stress, weaning stress, shearing stress, stress of regrouping, etc. have a significant impact on the welfare and health of the sheep as well as on the quantity of milk produced, its chemical composition, processing characteristics and the economic efficiency of the flock. In recent years, due to climate change, there has been an increasing number of studies on the effects of heat stress on the reproductive and production characteristics of sheep, as well as on their welfare and health. In addition, heat stress is often associated to the feed and the lack of water. The effects of stress are particularly pronounced in extensive sheep farming and milk production, which are prevalent in sheep farming. The aim of this paper is to describe the effects of the main types of stress on the quantity and chemical composition of sheep milk and on animal welfare.
{"title":"The influence of different stress factors on the production and quality of sheep milk","authors":"B. Mioč","doi":"10.15567/mljekarstvo.2024.0301","DOIUrl":"https://doi.org/10.15567/mljekarstvo.2024.0301","url":null,"abstract":"Around 10.5 million tonnes of sheep milk are produced worldwide, and around 3.1 million tonnes in Europe. In Europe, sheep milk production has stagnated slightly in recent years and currently accounts for around 1/3 of the global production. Due to its high content of dry matter, milk fat, total protein and casein, sheep milk is an excellent raw material for cheese production. Milk processing and cheese making are not only economically important, but also part of the cultural heritage based on a long tradition, often taking place in rural and less developed areas. Milk production and quality are very complex and are influenced by numerous factors, the most important of which are breed, feeding and body condition, parity and stage of lactation, milking and udder health, and environmental factors. In addition, different types of stress such as heat stress, nutritional stress, weaning stress, shearing stress, stress of regrouping, etc. have a significant impact on the welfare and health of the sheep as well as on the quantity of milk produced, its chemical composition, processing characteristics and the economic efficiency of the flock. In recent years, due to climate change, there has been an increasing number of studies on the effects of heat stress on the reproductive and production characteristics of sheep, as well as on their welfare and health. In addition, heat stress is often associated to the feed and the lack of water. The effects of stress are particularly pronounced in extensive sheep farming and milk production, which are prevalent in sheep farming. The aim of this paper is to describe the effects of the main types of stress on the quantity and chemical composition of sheep milk and on animal welfare.","PeriodicalId":18635,"journal":{"name":"Mljekarstvo","volume":null,"pages":null},"PeriodicalIF":1.2,"publicationDate":"2024-06-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141359904","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-06-11DOI: 10.15567/mljekarstvo.2024.0302
Z. Antunović
The research objective was to examine the influence of the first third of the lactation stage on 18 mineral elements in Alpine goat milk. The research was carried out on 20 goats of the French-Alpine breed, which were on average 5 years old and in the fourth lactation. The goats were monitored on the 30th and the 90th day of lactation. Their milk was analysed for chemical composition by the infrared spectroscopy. By means of inductively coupled plasma (ICP), a total of 18 minerals were analysed (Ca, P, K, Na, K, Mg, Fe, Zn, Cu, Se, Mn, Mo, Co, Cr, Ni, Cd, Pb, As and Hg). The period of milk sampling in the first third of lactation significantly influenced the changes in concentrations of Na, Se, Mo, Cr, Ni and As. Significantly higher concentrations of Na and As were determined in milk sampled on the 90th day, while lower concentrations of Se, Mo, Cr and Ni were determined in milk sampled on the 30th day of the first third of lactation. Analysis of the correlation coefficients between the investigated trace elements and the selected toxic elements in Alpine goat milk showed a significantly positive correlation between Ca:Mg, Ca:P, Ca:Co, Ca:Ni, Ca:Mo; Mg:P, Fe:As, Pb:Cd and P:Ni. In the first third of lactation, Alpine goat milk contained a very low concentration of heavy metals. The described changes indicate that Alpine goat milk is rich in essential elements, while the concentration of toxic elements is very low in the first third of lactation.
{"title":"Analysis of 18 elements in Alpine goat milk in the first third of lactation","authors":"Z. Antunović","doi":"10.15567/mljekarstvo.2024.0302","DOIUrl":"https://doi.org/10.15567/mljekarstvo.2024.0302","url":null,"abstract":"The research objective was to examine the influence of the first third of the lactation stage on 18 mineral elements in Alpine goat milk. The research was carried out on 20 goats of the French-Alpine breed, which were on average 5 years old and in the fourth lactation. The goats were monitored on the 30th and the 90th day of lactation. Their milk was analysed for chemical composition by the infrared spectroscopy. By means of inductively coupled plasma (ICP), a total of 18 minerals were analysed (Ca, P, K, Na, K, Mg, Fe, Zn, Cu, Se, Mn, Mo, Co, Cr, Ni, Cd, Pb, As and Hg). The period of milk sampling in the first third of lactation significantly influenced the changes in concentrations of Na, Se, Mo, Cr, Ni and As. Significantly higher concentrations of Na and As were determined in milk sampled on the 90th day, while lower concentrations of Se, Mo, Cr and Ni were determined in milk sampled on the 30th day of the first third of lactation. Analysis of the correlation coefficients between the investigated trace elements and the selected toxic elements in Alpine goat milk showed a significantly positive correlation between Ca:Mg, Ca:P, Ca:Co, Ca:Ni, Ca:Mo; Mg:P, Fe:As, Pb:Cd and P:Ni. In the first third of lactation, Alpine goat milk contained a very low concentration of heavy metals. The described changes indicate that Alpine goat milk is rich in essential elements, while the concentration of toxic elements is very low in the first third of lactation.","PeriodicalId":18635,"journal":{"name":"Mljekarstvo","volume":null,"pages":null},"PeriodicalIF":1.2,"publicationDate":"2024-06-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141359148","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-06-11DOI: 10.15567/mljekarstvo.2024.0303
Miroljub Barać
Lider cheese is a commercial full- fat semi-hard cheese produced from pasteurized goat`s milk in mountain area of Nikšić, Montenegro. The aim of this paper is to characterize the change of protein and fatty acid profiles, mineral content and antioxidant potential during the period of one month of its ripening. Proteolysis was monitored using electrophoresis of total extracts and water-soluble fractions under reducing conditions. Fatty acid profiles were determined by gas chromatography whereas the mineral content of cheese at different stage of ripening was determined by Inductively coupled plasma - optical emission spectrometry (ICP-OES) for macroelements and Inductively coupled plasma mass spectrometry (ICP-MS) for microelements, respectively. One month of ripening of Lider cheese is characterized by a relatively slow proteolysis which can be attributed to intensive lose of moisture, by the increase in desirable fatty acid content and consequently by the improvement of fatty acid healthy indices. After 30 days of ripening, mainly due to the degradation of αs-CN, the WSN/ TN and TCA/TN contents increased from 4.69 % to 6.06 % and 1.15 % to 1.90 %, respectively. One- month-old cheese had improved indexes of lipid quality as well as improved reducing power and ability to scavenge free radicals. Ripening did not affect mineral content of cheese if it is expressed on dry matter basis. According to obtained results, the optimal ripening period of Lider cheese is 15 days.
{"title":"Quality and healthy aspects of Lider cheese at different stages of ripening","authors":"Miroljub Barać","doi":"10.15567/mljekarstvo.2024.0303","DOIUrl":"https://doi.org/10.15567/mljekarstvo.2024.0303","url":null,"abstract":"Lider cheese is a commercial full- fat semi-hard cheese produced from pasteurized goat`s milk in mountain area of Nikšić, Montenegro. The aim of this paper is to characterize the change of protein and fatty acid profiles, mineral content and antioxidant potential during the period of one month of its ripening. Proteolysis was monitored using electrophoresis of total extracts and water-soluble fractions under reducing conditions. Fatty acid profiles were determined by gas chromatography whereas the mineral content of cheese at different stage of ripening was determined by Inductively coupled plasma - optical emission spectrometry (ICP-OES) for macroelements and Inductively coupled plasma mass spectrometry (ICP-MS) for microelements, respectively. One month of ripening of Lider cheese is characterized by a relatively slow proteolysis which can be attributed to intensive lose of moisture, by the increase in desirable fatty acid content and consequently by the improvement of fatty acid healthy indices. After 30 days of ripening, mainly due to the degradation of αs-CN, the WSN/ TN and TCA/TN contents increased from 4.69 % to 6.06 % and 1.15 % to 1.90 %, respectively. One- month-old cheese had improved indexes of lipid quality as well as improved reducing power and ability to scavenge free radicals. Ripening did not affect mineral content of cheese if it is expressed on dry matter basis. According to obtained results, the optimal ripening period of Lider cheese is 15 days.","PeriodicalId":18635,"journal":{"name":"Mljekarstvo","volume":null,"pages":null},"PeriodicalIF":1.2,"publicationDate":"2024-06-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141360387","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-06-11DOI: 10.15567/mljekarstvo.2024.0307
D. Tomanić
Mastitis is pressing concern for dairy herds due to its economic impact and potential health risks. Somatic cell counts (SCC), reflecting udder health, plays a crucial role in mastitis diagnosis. Current research explores the distribution of SCC and its correlation with various mastitis-causing pathogens in dairy farms in Serbia. The study analyzed 194 individual cow milk samples and microbiological testing was conducted under aseptic conditions to isolate and identify mastitis pathogens. The microscopic reference method was employed for assessing SCC in the milk samples. Among mastitis-associated isolates, bacteria were present in 28.87 %, yeast in 12.38 %, while in 5.15 % of milk samples, both bacteria and yeast were present. The relationship between SCC in various sample types (negative, bacteria-positive, yeast-positive, and samples with both bacteria and yeast) was noted. Importantly, samples with both bacteria and yeast presence had the highest SCC. While SCC is a valuable tool for monitoring udder health and the effectiveness of mastitis control programs, its response to specific pathogens is complex and doesn’t allow differentiation between pathogen types easily. This research highlights the challenges in distinguishing pathogen types based solely on SCC.
{"title":"Mastitis challenges in Serbian dairy farming: a study on somatic cell counts and pathogen distribution","authors":"D. Tomanić","doi":"10.15567/mljekarstvo.2024.0307","DOIUrl":"https://doi.org/10.15567/mljekarstvo.2024.0307","url":null,"abstract":"Mastitis is pressing concern for dairy herds due to its economic impact and potential health risks. Somatic cell counts (SCC), reflecting udder health, plays a crucial role in mastitis diagnosis. Current research explores the distribution of SCC and its correlation with various mastitis-causing pathogens in dairy farms in Serbia. The study analyzed 194 individual cow milk samples and microbiological testing was conducted under aseptic conditions to isolate and identify mastitis pathogens. The microscopic reference method was employed for assessing SCC in the milk samples. Among mastitis-associated isolates, bacteria were present in 28.87 %, yeast in 12.38 %, while in 5.15 % of milk samples, both bacteria and yeast were present. The relationship between SCC in various sample types (negative, bacteria-positive, yeast-positive, and samples with both bacteria and yeast) was noted. Importantly, samples with both bacteria and yeast presence had the highest SCC. While SCC is a valuable tool for monitoring udder health and the effectiveness of mastitis control programs, its response to specific pathogens is complex and doesn’t allow differentiation between pathogen types easily. This research highlights the challenges in distinguishing pathogen types based solely on SCC.","PeriodicalId":18635,"journal":{"name":"Mljekarstvo","volume":null,"pages":null},"PeriodicalIF":1.2,"publicationDate":"2024-06-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141355757","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-06-11DOI: 10.15567/mljekarstvo.2024.0305
Stefan Stepić
Buffalo milk, recognized for its rich composition, including higher levels of fat, lactose, protein, and ash when compared to cow milk, has been insufficiently studied considering its genetic diversity within the buffalo population in Serbia. This study focuses on the analysis of the CSN1S1 472G>C variant and its potential impact on rennet coagulation properties, critical for cheese production. Rennet coagulation parameters (rennet coagulation time (RCT), setting time (ST), aggregation rate (AR), and gel firmness (GF)), along with fresh curd yield (Yf), were examined in both, raw and high heat-treated buffalo milk. Genotyping results, obtained through Sanger sequencing of blood samples from 30 female buffaloes, revealed the presence of three CSN1S1 472G>C genotypes: GG, GC, and CC. The rheological measurements, conducted using dynamic small amplitude oscillatory rheology (SAOR), indicated significant statistical differences (p<0.05) among the tested parameters for these CSN1S1 472G>C genotypes in both raw and heat-treated buffalo milk. In addition, the influence of genotype on the yield of fresh curd in fresh and thermally treated milk was studied. These results can contribute to optimization in the process of production and processing of buffalo milk, emphasizing the importance of understanding the genetic factors in the composition of buffalo milk and its technological properties.
{"title":"Influence of αs1-casein genetic variant in buffalo milk and rennet coagulation properties","authors":"Stefan Stepić","doi":"10.15567/mljekarstvo.2024.0305","DOIUrl":"https://doi.org/10.15567/mljekarstvo.2024.0305","url":null,"abstract":"Buffalo milk, recognized for its rich composition, including higher levels of fat, lactose, protein, and ash when compared to cow milk, has been insufficiently studied considering its genetic diversity within the buffalo population in Serbia. This study focuses on the analysis of the CSN1S1 472G>C variant and its potential impact on rennet coagulation properties, critical for cheese production. Rennet coagulation parameters (rennet coagulation time (RCT), setting time (ST), aggregation rate (AR), and gel firmness (GF)), along with fresh curd yield (Yf), were examined in both, raw and high heat-treated buffalo milk. Genotyping results, obtained through Sanger sequencing of blood samples from 30 female buffaloes, revealed the presence of three CSN1S1 472G>C genotypes: GG, GC, and CC. The rheological measurements, conducted using dynamic small amplitude oscillatory rheology (SAOR), indicated significant statistical differences (p<0.05) among the tested parameters for these CSN1S1 472G>C genotypes in both raw and heat-treated buffalo milk. In addition, the influence of genotype on the yield of fresh curd in fresh and thermally treated milk was studied. These results can contribute to optimization in the process of production and processing of buffalo milk, emphasizing the importance of understanding the genetic factors in the composition of buffalo milk and its technological properties.","PeriodicalId":18635,"journal":{"name":"Mljekarstvo","volume":null,"pages":null},"PeriodicalIF":1.2,"publicationDate":"2024-06-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141356615","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-06-11DOI: 10.15567/mljekarstvo.2024.0306
Valentina Rahelić
The increase in childhood obesity is one of the biggest public health problems of the 21st century. Among the contributing dietary factors is the high consumption of sugar-sweetened beverages (SSB) and sweetened fruit juices (SFJ). On the other hand, regular consumption of milk and dairy products has decreased in many countries over the years, although research has shown an inverse association between dairy consumption and obesity. The aim of this study was to determine the effects of a dietary intervention within a multidisciplinary structured programme on reducing the consumption of SSB and SFJ and simultaneously increasing the consumption of milk and diary products in obese children/adolescents. The study included 100 participants of both sexes who had been diagnosed with obesity and participated in a five-day multidisciplinary structured programme focused on dietary intrevention. Standard anthropometric measurements and a validated questionnaire were used during the study. The mean age of the participants was 12.61±1.90 years, body mass index (BMI) was 98.38±1.26 percentiles; mean consumption of SSB was 1.68 ± 2.89 dL; SFJ was 3.72±5.09 dL and milk and dairy products was 3.10±2.35 dL per day at the start of the intervention. After the five-day programme, patients were reeducated once a month for the first six months, then every two months for up to 24 months. After 24 months, a significant decrease in SSB and SFJ intake (p<0.001) and an increase in milk and dairy products intake (p=0.002) were observed. The percentiles of the BMI also decreased after 24 months (p<0.001). The dietary intervention had a positive effect on the anthropometric values and the change in the dietary habits of the participants, with a focus on the consumption of milk and dairy products.
{"title":"Role of dietary intervention on sugar-sweetened beverages, fruit juices and milk consumption among obese children/adolescents","authors":"Valentina Rahelić","doi":"10.15567/mljekarstvo.2024.0306","DOIUrl":"https://doi.org/10.15567/mljekarstvo.2024.0306","url":null,"abstract":"The increase in childhood obesity is one of the biggest public health problems of the 21st century. Among the contributing dietary factors is the high consumption of sugar-sweetened beverages (SSB) and sweetened fruit juices (SFJ). On the other hand, regular consumption of milk and dairy products has decreased in many countries over the years, although research has shown an inverse association between dairy consumption and obesity. The aim of this study was to determine the effects of a dietary intervention within a multidisciplinary structured programme on reducing the consumption of SSB and SFJ and simultaneously increasing the consumption of milk and diary products in obese children/adolescents. The study included 100 participants of both sexes who had been diagnosed with obesity and participated in a five-day multidisciplinary structured programme focused on dietary intrevention. Standard anthropometric measurements and a validated questionnaire were used during the study. The mean age of the participants was 12.61±1.90 years, body mass index (BMI) was 98.38±1.26 percentiles; mean consumption of SSB was 1.68 ± 2.89 dL; SFJ was 3.72±5.09 dL and milk and dairy products was 3.10±2.35 dL per day at the start of the intervention. After the five-day programme, patients were reeducated once a month for the first six months, then every two months for up to 24 months. After 24 months, a significant decrease in SSB and SFJ intake (p<0.001) and an increase in milk and dairy products intake (p=0.002) were observed. The percentiles of the BMI also decreased after 24 months (p<0.001). The dietary intervention had a positive effect on the anthropometric values and the change in the dietary habits of the participants, with a focus on the consumption of milk and dairy products.","PeriodicalId":18635,"journal":{"name":"Mljekarstvo","volume":null,"pages":null},"PeriodicalIF":1.2,"publicationDate":"2024-06-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141357294","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-06-11DOI: 10.15567/mljekarstvo.2024.0304
Nina Čuljak
The presence of surface (S)-layer proteins as a rare property of lactic acid bacteria plays an important role in the expression of the probiotic properties of the producer strain and protects the cells from unfavourable environmental conditions. Therefore, the aim of this work was to evaluate the role of S-layer proteins of four Levilactobacillus brevis MB1, MB2, MB13, and MB20 strains, previously isolated from human milk. The results showed better survival under simulated gastrointestinal tract (GIT) conditions and better adhesion to Caco-2 cells, achieved when S-proteins were present on the surface of the examined probiotic Levilactobacillus brevis strains. Free cells and cells microencapsulated in alginate, with or without galactooligosaccharides (GOS) and fructooligosaccharides (FOS), survived in high numbers during freeze-drying and one-month storage at 4 °C, as well as during exposure to simulated GIT conditions (>108 CFU/g), thus fulfilling the primary technological criterion for the selection of probiotic strains. Higher survival of freeze-drying of encapsulated cells than free cells, can be attributed to the protective effect of alginate, GOS and FOS as prebiotic agents. However, GOS was more protective than FOS in maintaining the viability of microencapsulated Levilactobacillus brevis MB1, MB2, MB13 and MB20 cells in alginate during storage and under the harsh conditions of GIT and freeze-drying.
表面（S）层蛋白的存在是乳酸菌的一种罕见特性，在表达生产菌株的益生特性和保护细胞免受不利环境条件影响方面发挥着重要作用。因此，这项工作的目的是评估之前从人奶中分离出来的四种左旋乳杆菌 MB1、MB2、MB13 和 MB20 菌株的 S 层蛋白的作用。研究结果表明，在模拟胃肠道（GIT）条件下，当 S 蛋白存在于受检的益生菌左旋乳杆菌菌株表面时，其存活率更高，与 Caco-2 细胞的粘附性更好。游离细胞和海藻酸盐微囊化细胞，无论是否含有半乳寡糖（GOS）和果寡糖（FOS），在冷冻干燥和 4 °C 下储存一个月期间，以及在模拟胃肠道条件下（>108 CFU/g），都有很高的存活率，从而满足了选择益生菌株的主要技术标准。与游离细胞相比，封装细胞的冻干存活率更高，这可能是由于海藻酸盐、GOS 和 FOS 作为益生元剂的保护作用。不过，在海藻酸盐中微胶囊化的保加利亚左旋乳杆菌 MB1、MB2、MB13 和 MB20 细胞在储存期间以及在胃肠道和冷冻干燥的苛刻条件下保持存活率方面，GOS 比 FOS 更有保护作用。
{"title":"S-layer proteins in microencapsulated freeze-dried probiotic Levilactobacillus brevis strains","authors":"Nina Čuljak","doi":"10.15567/mljekarstvo.2024.0304","DOIUrl":"https://doi.org/10.15567/mljekarstvo.2024.0304","url":null,"abstract":"The presence of surface (S)-layer proteins as a rare property of lactic acid bacteria plays an important role in the expression of the probiotic properties of the producer strain and protects the cells from unfavourable environmental conditions. Therefore, the aim of this work was to evaluate the role of S-layer proteins of four Levilactobacillus brevis MB1, MB2, MB13, and MB20 strains, previously isolated from human milk. The results showed better survival under simulated gastrointestinal tract (GIT) conditions and better adhesion to Caco-2 cells, achieved when S-proteins were present on the surface of the examined probiotic Levilactobacillus brevis strains. Free cells and cells microencapsulated in alginate, with or without galactooligosaccharides (GOS) and fructooligosaccharides (FOS), survived in high numbers during freeze-drying and one-month storage at 4 °C, as well as during exposure to simulated GIT conditions (>108 CFU/g), thus fulfilling the primary technological criterion for the selection of probiotic strains. Higher survival of freeze-drying of encapsulated cells than free cells, can be attributed to the protective effect of alginate, GOS and FOS as prebiotic agents. However, GOS was more protective than FOS in maintaining the viability of microencapsulated Levilactobacillus brevis MB1, MB2, MB13 and MB20 cells in alginate during storage and under the harsh conditions of GIT and freeze-drying.","PeriodicalId":18635,"journal":{"name":"Mljekarstvo","volume":null,"pages":null},"PeriodicalIF":1.2,"publicationDate":"2024-06-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141355408","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-03-25DOI: 10.15567/mljekarstvo.2024.0204
Mustafa Şengül
The aroma of fermented dairy products is greatly influenced by starter cultures and by the lactic acid bacteria strains that are used as starter cultures. Experimental butter samples were produced using no starter culture, by a commercial starter culture (Lactococcus lactis subsp. lactis, Lactococcus lactis subsp. cremoris, Lactococcus lactis subsp. lactis biovar. diacetylactis, Leuconostoc mesenteroides), by a prepared bacteria mix (Lactococcus lactis subsp. lactis EM1, Lactococcus lactis subsp. cremoris EM28, Lactococcus lactis subsp. lactis biovar. diacetylactis G49, Leuconostoc mesenteroides B3), and by a prepared bacteria mix fortified with Lactobacillus rhamnosus B19 (L. rhamnosus B19). The aroma profile of the butter samples was investigated using a gas chromatography mass spectometry method (GC-MS). A total of 92 components were determined in the butter samples, including acids (12), alcohols (20), aldehydes (9), alkanes (6), esters (8), ketones (19) and several other aroma compounds (18). The sample containing the prepared starter culture had the most detectable aroma components, while the lowest number of aromatic components were found in the sample without a starter culture. 2,3-butanedione (diacetyl) was not detected in the sample without a starter culture. 2-Butanone 3-hydroxy- (acetoin) content was the highest in the sample containing the commercial starter culture and the lowest in the sample without a starter culture.
{"title":"The aroma profile of butter produced using different starter cultures","authors":"Mustafa Şengül","doi":"10.15567/mljekarstvo.2024.0204","DOIUrl":"https://doi.org/10.15567/mljekarstvo.2024.0204","url":null,"abstract":"The aroma of fermented dairy products is greatly influenced by starter cultures and by the lactic acid bacteria strains that are used as starter cultures. Experimental butter samples were produced using no starter culture, by a commercial starter culture (Lactococcus lactis subsp. lactis, Lactococcus lactis subsp. cremoris, Lactococcus lactis subsp. lactis biovar. diacetylactis, Leuconostoc mesenteroides), by a prepared bacteria mix (Lactococcus lactis subsp. lactis EM1, Lactococcus lactis subsp. cremoris EM28, Lactococcus lactis subsp. lactis biovar. diacetylactis G49, Leuconostoc mesenteroides B3), and by a prepared bacteria mix fortified with Lactobacillus rhamnosus B19 (L. rhamnosus B19). The aroma profile of the butter samples was investigated using a gas chromatography mass spectometry method (GC-MS). A total of 92 components were determined in the butter samples, including acids (12), alcohols (20), aldehydes (9), alkanes (6), esters (8), ketones (19) and several other aroma compounds (18). The sample containing the prepared starter culture had the most detectable aroma components, while the lowest number of aromatic components were found in the sample without a starter culture. 2,3-butanedione (diacetyl) was not detected in the sample without a starter culture. 2-Butanone 3-hydroxy- (acetoin) content was the highest in the sample containing the commercial starter culture and the lowest in the sample without a starter culture.","PeriodicalId":18635,"journal":{"name":"Mljekarstvo","volume":null,"pages":null},"PeriodicalIF":1.2,"publicationDate":"2024-03-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140382032","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-03-25DOI: 10.15567/mljekarstvo.2024.0202
Cem Karagözlü
Probiotic fermented milks were produced from goat milk containing two different somatic cell counts (SCC) at low (<500.000 cells/mL) and high (>1.500.000 cells/mL) levels. The produced samples were analysed for some chemical, textural, microbiological and sensory properties. The samples made from high SCC goat milk had higher lipolytic activity. The viscosity and hardness of probiotic fermented milks with low SCC were higher than that of the higher SCC samples. Probiotic fermented milks produced with low SCC achieved higher sensory scores and the viability of the starter bacteria was more stable in these samples.
{"title":"Characterisation of probiotic fermented milks produced from goat milk containing low or high somatic cell counts","authors":"Cem Karagözlü","doi":"10.15567/mljekarstvo.2024.0202","DOIUrl":"https://doi.org/10.15567/mljekarstvo.2024.0202","url":null,"abstract":"Probiotic fermented milks were produced from goat milk containing two different somatic cell counts (SCC) at low (<500.000 cells/mL) and high (>1.500.000 cells/mL) levels. The produced samples were analysed for some chemical, textural, microbiological and sensory properties. The samples made from high SCC goat milk had higher lipolytic activity. The viscosity and hardness of probiotic fermented milks with low SCC were higher than that of the higher SCC samples. Probiotic fermented milks produced with low SCC achieved higher sensory scores and the viability of the starter bacteria was more stable in these samples.","PeriodicalId":18635,"journal":{"name":"Mljekarstvo","volume":null,"pages":null},"PeriodicalIF":1.2,"publicationDate":"2024-03-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140384911","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2024-03-25DOI: 10.15567/mljekarstvo.2024.0201
Irena Barukčić Jurina
Mare milk is known for therapeutic properties due to the presence of bioactive components that are sensitive to thermal treatments and tend to lose their activity. Accordingly, mare milk is consumed raw or frozen, but for food safety reasons recent studies suggest mandatory pasteurization of mare milk before consumption. The aim of this study was to examine the effects of different heat treatments on mare milk quality and compare those to heat induced changes in cow milk. In all milk samples, the total dissolved solids (TDS), electrical conductivity (EC), antioxidant capacity (AC) by the DPPH and FRAP methods, changes in colour (ΔE*), concentration of 5-hydroxymethylfurfural (HMF) and the total phenols (TPC) were determined. Heat induced protein denaturation was monitored by SDS-PAGE, while NIR was applied for qualitative analysis of structural changes. In-bottle sterilization at 116 °C/16ʹ and 120 °C/5ʹ showed to be inappropriate for mare milk processing, since they resulted in the most intense colour changes (ΔE*>5), the highest free and total HMF concentrations (40.70 μmol/L free, 70.26 μmol/L total HMF at 116 °C/16ʹ; 35.49 μmol/L free, 58.12 μmol/L total HMF at 120 °C/5ʹ) and the most intense protein denaturation. On contrary, the applied HTST regimes (72 °C/20”, 85 °C/30”, 90 °C/5ʹ) and thermisation at 63 °C/15” were appropriate for mare milk processing considering the studied qualitative changes. Thereby, the HMF concentration, FRAP, TPC and ΔE* value, combined with NIR analysis showed a good potential to be used as rapid and useful tools for monitoring the intensity of heat induced changes in mare milk.
{"title":"Evaluation of heat-induced changes and 5-hydroxymethylfurfural formation in mare milk - a comparative study to cow milk","authors":"Irena Barukčić Jurina","doi":"10.15567/mljekarstvo.2024.0201","DOIUrl":"https://doi.org/10.15567/mljekarstvo.2024.0201","url":null,"abstract":"Mare milk is known for therapeutic properties due to the presence of bioactive components that are sensitive to thermal treatments and tend to lose their activity. Accordingly, mare milk is consumed raw or frozen, but for food safety reasons recent studies suggest mandatory pasteurization of mare milk before consumption. The aim of this study was to examine the effects of different heat treatments on mare milk quality and compare those to heat induced changes in cow milk. In all milk samples, the total dissolved solids (TDS), electrical conductivity (EC), antioxidant capacity (AC) by the DPPH and FRAP methods, changes in colour (ΔE*), concentration of 5-hydroxymethylfurfural (HMF) and the total phenols (TPC) were determined. Heat induced protein denaturation was monitored by SDS-PAGE, while NIR was applied for qualitative analysis of structural changes. In-bottle sterilization at 116 °C/16ʹ and 120 °C/5ʹ showed to be inappropriate for mare milk processing, since they resulted in the most intense colour changes (ΔE*>5), the highest free and total HMF concentrations (40.70 μmol/L free, 70.26 μmol/L total HMF at 116 °C/16ʹ; 35.49 μmol/L free, 58.12 μmol/L total HMF at 120 °C/5ʹ) and the most intense protein denaturation. On contrary, the applied HTST regimes (72 °C/20”, 85 °C/30”, 90 °C/5ʹ) and thermisation at 63 °C/15” were appropriate for mare milk processing considering the studied qualitative changes. Thereby, the HMF concentration, FRAP, TPC and ΔE* value, combined with NIR analysis showed a good potential to be used as rapid and useful tools for monitoring the intensity of heat induced changes in mare milk.","PeriodicalId":18635,"journal":{"name":"Mljekarstvo","volume":null,"pages":null},"PeriodicalIF":1.2,"publicationDate":"2024-03-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140381474","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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