L-ASPARAGINASE ENZYME PRODUCTION FROM MARINE FUNGUS BEAUVERIA BASSIANA – STATISTICAL OPTIMIZATION

Abstract

Statistical optimization was done to enhance the production of L-asparaginase by submerged fermentation from the marine fungus Beauveria bassiana, which was isolated from sediments and corals of sea. Optimization of different process parameters for the production of L-asparaginase and validation using response surface methodology involving central composite design (CCD) was performed with four variables, carbon source, nitrogen source which are essentially important for the growth of the microorganism and other conditions like pH and inoculum level, which include dextrose at 0.43% w/V, L-asparagine 1.1%w/V, pH 7.5 and an inoculum level of 10.2% w/V, respectively. Extracellular production of L-asparaginase by B. bassiana and its potential for L-asparaginase production is reported in the present study.