DEVELOPMENT OF MICROSATELLITE MARKERS FOR Balfourodendron riedelianum (Engl.) Engl. (PAU MARFIM - IVORY TREE): AN ENDANGERED HIGH VALUE TROPICAL TREE (SCIENTIFIC NOTE)

IF 0.1 0 LITERATURE, ROMANCE Revista do Instituto Florestal Pub Date : 2019-12-20 DOI:10.24278/2178-5031.201931206
B. I. Aguiar, F. Gandara, M. Ambrosano, Paulo Yoshio Kageyama, A. M. Sebbenn, Miguel Luiz Menezes Freitas, E. A. Silvestre, K. Schwarcz, M. Zucchi, M. D. Moraes, Dario Grattapsglia
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Abstract

Pressure on tropical forests by agriculture and livestock expansion, frequently leads to highly fragmented and isolated populations. Limited gene fow drives increased drift and genetic differentiation among populations, ultimately reducing the overall genetic diversity of forest tree species. Balforoudendron riedelianum (Engl.) Engl., commonly known as pau marfm (ivory tree), a valuable Brazilian hardwood tree used in carpentry and building, is currently endangered due to logging and forest fragmentation. Information on genetic diversity and structure of remnants populations is necessary to support its conservation and sustainable management. Microsatellite markers are an effective tool for understanding and quantifying the effects of fragmentation on genetic diversity. Seven microsatellite markers were developed and validated using a sample of 98 individuals. The number of alleles per locus ranged from 3 to 25, the observed and expected heterozygosities from 0.051 to 0.909 and 0.050 to 0.930, respectively, the fxation index corrected for null alleles from 0.036 to 1.0 and all markers were found in linkage equilibrium. This microsatellite marker set is suitable to estimate population genetic parameters in support of sustainable management and conservation, and to assess relatedness and parentage in breeding populations.
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巴尔福登(Balfourodendron riedelanum)微卫星标记的开发心血管病。(pau marfim -象牙树):濒危的高价值热带乔木(科学注释)
农业和畜牧业的扩张给热带森林带来压力,往往导致种群高度分散和孤立。有限的基因流动增加了种群间的漂移和遗传分化,最终降低了森林树种的整体遗传多样性。牛皮桐(英国)心血管病。它通常被称为pau marfm(象牙树),是一种珍贵的巴西硬木树,用于木工和建筑,目前由于伐木和森林破碎化而濒临灭绝。残存种群的遗传多样性和结构信息对于支持其保护和可持续管理是必要的。微卫星标记是了解和量化碎片化对遗传多样性影响的有效工具。开发了7个微卫星标记,并使用98个个体的样本进行了验证。每个位点的等位基因数为3 ~ 25个,观察杂合度和期望杂合度分别为0.051 ~ 0.909和0.050 ~ 0.930,零等位基因校正的固定指数为0.036 ~ 1.0,所有标记均处于连锁平衡。该微卫星标记集可用于估计种群遗传参数,以支持种群的可持续管理和保护,并可用于评估育种群体的亲缘关系和亲本关系。
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