Analysis of the transcriptome and metabolome reveals phenylpropanoid mechanism in common bean (Phaseolus vulgaris) responding to salt stress at sprout stage

IF 4 2区 农林科学 Q2 FOOD SCIENCE & TECHNOLOGY Food and Energy Security Pub Date : 2023-09-01 DOI:10.1002/fes3.481
Qi Zhang, Shukun Wang, Bin Qin, Hao-yue Sun, Xian-kai Yuan, Qi Wang, Junjie Xu, Zhengong Yin, Yan-li Du, Ji-dao Du, Caihua Li
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Abstract

Common bean (Phaseolus vulgaris) seeds are important legume crops and an important source of dietary proteins and carbohydrates. Therefore, it is important to develop strategies to improve salt tolerance in common beans. In this study, transcriptome and metabolome analyses were conducted on local common bean variety under salt stress at the sprout stage for a period of 0, 12, and 24 h. Results showed that phenylpropanoid pathways (including phenylpropanoid biosynthesis and phenylalanine metabolism) and flavonoid pathways (including flavonoid biosynthesis and flavone and flavonol biosynthesis) played an important role in controlling responses to salt stress as evidenced by analysis of differentially expression genes, common expression patterns, WCGNA, and differentially altered metabolites (DAMs) analyses. In addition, exploration of the activities of 4-coumarate-CoA ligase (4CL), caffeoyl-CoA O-methyltransferase (CCoAOMT), peroxidase (POD), chalcone isomerase (CHI), dihydroflavonol-4-reductase (DFR), and flavonol synthase (FLS) further showed that phenylpropanoid and flavonoid pathways participate in plant responses to salt stress. Moreover, the phenylpropanoid pathways and flavonoid pathways were found to be potential pathways regulating plant response to salt stress based on transcriptome and metabolome analysis. The activities of 4CL, CCoAOMT, POD, CHI, DFR, and FLS revealed that these pathways are crucial to the regulation of plant responses to salt stress. These findings provided theoretical basis for further improvement of salt tolerance in common bean.

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通过转录组学和代谢组学分析,揭示了豆芽期苯丙素对盐胁迫的响应机制
蚕豆种子是重要的豆科作物,也是膳食蛋白质和碳水化合物的重要来源。因此,制定提高普通豆耐盐性的策略具有重要意义。本研究对盐胁迫下当地普通豆品种发芽期0、12和24 h的转录组和代谢组进行了分析。结果表明,通过差异表达基因、共同表达模式、WCGNA和差异改变代谢物(DAMs)分析,苯丙素途径(包括苯丙素生物合成和苯丙氨酸代谢)和黄酮途径(包括类黄酮生物合成和黄酮和黄酮醇生物合成)在盐胁迫响应中发挥重要作用。此外,对4‐香豆酸‐辅酶a连接酶(4CL)、咖啡酰‐辅酶a O‐甲基转移酶(CCoAOMT)、过氧化物酶(POD)、查尔酮异构酶(CHI)、二氢黄酮醇‐4‐还原酶(DFR)和黄酮醇合成酶(FLS)活性的研究进一步表明,苯丙酸途径和类黄酮途径参与了植物对盐胁迫的响应。此外,通过转录组和代谢组分析发现,苯丙素途径和类黄酮途径是调控植物对盐胁迫响应的潜在途径。4CL、CCoAOMT、POD、CHI、DFR和FLS的活性揭示了这些途径在调控植物对盐胁迫的响应中起重要作用。这些研究结果为进一步提高普通豆的耐盐性提供了理论依据。
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来源期刊
Food and Energy Security
Food and Energy Security Energy-Renewable Energy, Sustainability and the Environment
CiteScore
9.30
自引率
4.00%
发文量
76
审稿时长
19 weeks
期刊介绍: Food and Energy Security seeks to publish high quality and high impact original research on agricultural crop and forest productivity to improve food and energy security. It actively seeks submissions from emerging countries with expanding agricultural research communities. Papers from China, other parts of Asia, India and South America are particularly welcome. The Editorial Board, headed by Editor-in-Chief Professor Martin Parry, is determined to make FES the leading publication in its sector and will be aiming for a top-ranking impact factor. Primary research articles should report hypothesis driven investigations that provide new insights into mechanisms and processes that determine productivity and properties for exploitation. Review articles are welcome but they must be critical in approach and provide particularly novel and far reaching insights. Food and Energy Security offers authors a forum for the discussion of the most important advances in this field and promotes an integrative approach of scientific disciplines. Papers must contribute substantially to the advancement of knowledge. Examples of areas covered in Food and Energy Security include: • Agronomy • Biotechnological Approaches • Breeding & Genetics • Climate Change • Quality and Composition • Food Crops and Bioenergy Feedstocks • Developmental, Physiology and Biochemistry • Functional Genomics • Molecular Biology • Pest and Disease Management • Post Harvest Biology • Soil Science • Systems Biology
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