Identification of Pathogenic Bacteria in Blood Cockle (Anadara granosa) using 16S rRNA Gene

Abstract

Blood cockle (Anadara granosa) is one of the marine resources in Indonesia that contains protein. Processing of blood cockles that are not perfect or raw will be contaminated with pathogenic bacteria that live in the waters. Pathogenic bacteria cause foodborne disease, which is a disease in humans caused by food. Several bacterial pathogens that cause foodborne disease are Escherichia sp. Pseudomonas sp., and Vibrio sp. Pathogenic bacteria in blood cockle should be identified using 16S rRNA as molecular identification. Samples were isolated using BAP, HIA, and BHI media. Bacteria from BHI media were isolated. Isolation DNA was isolated using the phenol-CIAA method. The DNA isolates were amplified by the PCR method based on the 16S rRNA target gene, then visualized the DNA with 2% agarose gel electrophoresis and sequencing. Bacterial colonies produced from BAP media for isolates BVA1, BVA9, and BVA10 were ß-hemolysis. Visualization of hemolytic bacterial DNA in blood cockle culture amplified about 1500 bp. Whereas the results of the sequencing analyzed by BLAST on the NCBI database and the Mega X program for BVA1 and BVA10 isolates showed similarity to Vibrio sp. bacteria, whereas BVA9 isolates showed similarity to Bacterium whose species still unknown. The conclusion showed that blood cockle had close simililarity with with Vibrio sp. and Bacterium.