Coffee is a drink that is commonly consumed by Indonesian people. Coffee contains caffeine, cafestol, and kahweol which can increase lipid levels, including increasing triglyceride levels. Triglycerides are substances consisting of glycerol linked to fatty acid groups. The purpose of this study was to describe blood triglyceride levels in coffee addict students at Anwar Medika University using the GPO-PAP method. This study uses a quantitative descriptive design with a cross-sectional survey approach. This research was conducted in May 2023. The research sample consisted of 48 samples which were determined using a purposive sampling technique which was taken at random. The univariate analysis used is the distribution of frequencies and percentages which describe the presentation of data for one variable. This research test obtained an average triglyceride level of 158.7 mg/dl. Based on age category, maximum triglyceride levels were 204.73 mg/dl with an average of 152.26 mg/dl. Based on lifestyle, the maximum triglyceride level is 189.93 mg/dl with an average of 166.43 mg/dl. Based on the frequency of coffee consumption, the maximum triglyceride level is 160.64 mg/dl with an average of 158.51 mg/dl. Based on physical activity, the maximum triglyceride level is 173.88 mg/dl with an average of 160.60 mg/dl. Based on the type of coffee consumed, the maximum triglyceride level was 166.76 mg/dl with an average of 164.57 mg/dl. So, it can be concluded that excessive coffee consumption can increase triglyceride levels in the body.
{"title":"Overview of trygleseride level on coffee consumption","authors":"Amellya Octifani, Tarisa Suci Novianti, Farida Anwari, Arif Rahman Nurdianto, Fery Setiawan, Rizal Fauzi Nurdianto","doi":"10.29238/teknolabjournal.v12i2.427","DOIUrl":"https://doi.org/10.29238/teknolabjournal.v12i2.427","url":null,"abstract":"Coffee is a drink that is commonly consumed by Indonesian people. Coffee contains caffeine, cafestol, and kahweol which can increase lipid levels, including increasing triglyceride levels. Triglycerides are substances consisting of glycerol linked to fatty acid groups. The purpose of this study was to describe blood triglyceride levels in coffee addict students at Anwar Medika University using the GPO-PAP method. This study uses a quantitative descriptive design with a cross-sectional survey approach. This research was conducted in May 2023. The research sample consisted of 48 samples which were determined using a purposive sampling technique which was taken at random. The univariate analysis used is the distribution of frequencies and percentages which describe the presentation of data for one variable. This research test obtained an average triglyceride level of 158.7 mg/dl. Based on age category, maximum triglyceride levels were 204.73 mg/dl with an average of 152.26 mg/dl. Based on lifestyle, the maximum triglyceride level is 189.93 mg/dl with an average of 166.43 mg/dl. Based on the frequency of coffee consumption, the maximum triglyceride level is 160.64 mg/dl with an average of 158.51 mg/dl. Based on physical activity, the maximum triglyceride level is 173.88 mg/dl with an average of 160.60 mg/dl. Based on the type of coffee consumed, the maximum triglyceride level was 166.76 mg/dl with an average of 164.57 mg/dl. So, it can be concluded that excessive coffee consumption can increase triglyceride levels in the body.","PeriodicalId":31934,"journal":{"name":"Jurnal Teknologi Laboratorium","volume":"59 7","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-12-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139009602","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-11-04DOI: 10.29238/teknolabjournal.v12i2.444
Yenry Sumarlim, Emilia Gan, Desantika Wuryana, M. H. Pristantiningtyas, Herya Putra Dharma, Muhammad Muchlis, Jainuri Erik Pratama, A. P. Setiadi, M. Gondokesumo
Chronic Kidney Disease (CKD) is closely related to hypertension. Increasing the severity of CKD is associated with more difficult blood pressure control. Appropriate therapeutic management is needed to prevent complications due to uncontrolled hypertension. We report the case of a 78-year-old female patient with a diagnosis of hypertension and end-stage CKD with a history of undergoing hemodialysis for 4 years. The patient has been taking antihypertensive drugs such as Angiotensin Receptor Blockers, Calcium Channel Blockers and Diuretics. However, the administration of three antihypertensive drugs still could not help achieve the expected blood pressure target where the systolic blood pressure was still above 160 mmHg. The patient also has anemia as a common complication of chronic kidney disease. Appropriate management of therapy with fourth-line therapy and hemoglobin repair is necessary to achieve improved clinical outcomes and reduce renal worsening.
{"title":"Therapy of resistant hypertension in patients with chronic kidney disease complications of anemia in hemodialysis: a case report","authors":"Yenry Sumarlim, Emilia Gan, Desantika Wuryana, M. H. Pristantiningtyas, Herya Putra Dharma, Muhammad Muchlis, Jainuri Erik Pratama, A. P. Setiadi, M. Gondokesumo","doi":"10.29238/teknolabjournal.v12i2.444","DOIUrl":"https://doi.org/10.29238/teknolabjournal.v12i2.444","url":null,"abstract":"Chronic Kidney Disease (CKD) is closely related to hypertension. Increasing the severity of CKD is associated with more difficult blood pressure control. Appropriate therapeutic management is needed to prevent complications due to uncontrolled hypertension. We report the case of a 78-year-old female patient with a diagnosis of hypertension and end-stage CKD with a history of undergoing hemodialysis for 4 years. The patient has been taking antihypertensive drugs such as Angiotensin Receptor Blockers, Calcium Channel Blockers and Diuretics. However, the administration of three antihypertensive drugs still could not help achieve the expected blood pressure target where the systolic blood pressure was still above 160 mmHg. The patient also has anemia as a common complication of chronic kidney disease. Appropriate management of therapy with fourth-line therapy and hemoglobin repair is necessary to achieve improved clinical outcomes and reduce renal worsening.","PeriodicalId":31934,"journal":{"name":"Jurnal Teknologi Laboratorium","volume":"1200 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-11-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139289479","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-09-17DOI: 10.29238/teknolabjournal.v12i2.408
M. Gondokesumo, F. A. Muslikh, Ni Putu Diah Nopitasari, Putu Dea Angelita Putri
The prevalence of Parkinson's disease is increasing every year. This progressive disease is characterized by the loss of neurons in the substantia nigra due to the presence of alpha-synuclein aggregates. Keluwih leaves (Artocarpus camansi) are known to have activity in inhibiting acetylcholinesterase, as well as being an antioxidant and anti-inflammatory. The aim of this study was to evaluate the effect of ethanol extract of A. camansi leaves on the levels of alpha-synuclein in male and female adult zebrafish induced with rotenone. The zebrafish were induced with rotenone at a concentration of 5 µg/L for 28 days, along with the administration of 96% ethanol extract of A. camansi leaves at doses of 2.5, 5, 7.5, or 10 mg/L. The media was changed every 48 hours to maintain the concentration of rotenone and extract. After 28 days, alpha-synuclein levels were examined using immunohistochemistry. The administration of ethanol extract of A. camansi leaves can reduce the average levels of alpha-synuclein in male and female adult zebrafish, with the optimum dose being 2.5 mg/L. Therefore, it can be concluded that the administration of ethanol extract of A. camansi leaves can be used as an alternative treatment for Parkinson's disease.
{"title":"Decreasing alpha-synuclein aggregation on rotenone-induced adult Zebrafish as Parkinson’s Diseases model by ethanol extraction of Keluwih (Artocarpus camansi) leaves","authors":"M. Gondokesumo, F. A. Muslikh, Ni Putu Diah Nopitasari, Putu Dea Angelita Putri","doi":"10.29238/teknolabjournal.v12i2.408","DOIUrl":"https://doi.org/10.29238/teknolabjournal.v12i2.408","url":null,"abstract":"The prevalence of Parkinson's disease is increasing every year. This progressive disease is characterized by the loss of neurons in the substantia nigra due to the presence of alpha-synuclein aggregates. Keluwih leaves (Artocarpus camansi) are known to have activity in inhibiting acetylcholinesterase, as well as being an antioxidant and anti-inflammatory. The aim of this study was to evaluate the effect of ethanol extract of A. camansi leaves on the levels of alpha-synuclein in male and female adult zebrafish induced with rotenone. The zebrafish were induced with rotenone at a concentration of 5 µg/L for 28 days, along with the administration of 96% ethanol extract of A. camansi leaves at doses of 2.5, 5, 7.5, or 10 mg/L. The media was changed every 48 hours to maintain the concentration of rotenone and extract. After 28 days, alpha-synuclein levels were examined using immunohistochemistry. The administration of ethanol extract of A. camansi leaves can reduce the average levels of alpha-synuclein in male and female adult zebrafish, with the optimum dose being 2.5 mg/L. Therefore, it can be concluded that the administration of ethanol extract of A. camansi leaves can be used as an alternative treatment for Parkinson's disease.","PeriodicalId":31934,"journal":{"name":"Jurnal Teknologi Laboratorium","volume":"2014 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-09-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139339389","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-08-25DOI: 10.29238/teknolabjournal.v12i1.403
Amellya Octifani, Budi Santosa, Junaedi Wibawa
COVID-19 is a global health problem that is divided into mild, moderate, and severe degrees of severity and a high mortality rate. The coagulopathy system has value in COVID-19 patients. This study aims to determine the relationship and predictive value of d-dimer, PT, aPTT, and albumin with severity and mortality in COVID-19 positive patients. The type of research used is retrospective with a cross-sectional approach. The research data were taken using a simple random sampling technique from the Medical Record Installation of Husada Utama Hospital, Surabaya in August-September 2021. Bivariate relationship data analysis used the Chi-Square test followed by a multivariate logistic regression test with cut-off values ??of D-dimer, PT, aPTT, and albumin each 0.5 g/mL, 14.0 sec. 36.0 seconds and 3.5 g/dL. The results of the chi-square test (? < 0.05) showed the sig value of D-Dimer, PT, aPTT albumin with a severity level of 0.000; 0.000; 0.001; 0.001 while the value of Sig. with a mortality of 0.000; 0.047; 0.239; 0.022. The results of the multivariate logistic regression test with the degree of severity obtained the value of Sig. 0.000; 0.000; 0.021; 0.000 with a [PR] value of 16.7; 4.4; 2.7; 14.4. The results of the multivariate logistic regression test with mortality obtained the value of Sig. 0.000; 0.020; 0.273 with a [PR] value of 26.9; 2.8;1.6. There is a relationship between D-Dimer, PT, aPTT, and albumin with severity and mortality and can be used as a predictor of severity and mortality in COVID-19 patients.
{"title":"Relationship of D-Dimer, Pt, Aptt, and Albumin with severity and mortality rate in Covid-19 positive patients","authors":"Amellya Octifani, Budi Santosa, Junaedi Wibawa","doi":"10.29238/teknolabjournal.v12i1.403","DOIUrl":"https://doi.org/10.29238/teknolabjournal.v12i1.403","url":null,"abstract":"COVID-19 is a global health problem that is divided into mild, moderate, and severe degrees of severity and a high mortality rate. The coagulopathy system has value in COVID-19 patients. This study aims to determine the relationship and predictive value of d-dimer, PT, aPTT, and albumin with severity and mortality in COVID-19 positive patients. The type of research used is retrospective with a cross-sectional approach. The research data were taken using a simple random sampling technique from the Medical Record Installation of Husada Utama Hospital, Surabaya in August-September 2021. Bivariate relationship data analysis used the Chi-Square test followed by a multivariate logistic regression test with cut-off values ??of D-dimer, PT, aPTT, and albumin each 0.5 g/mL, 14.0 sec. 36.0 seconds and 3.5 g/dL. The results of the chi-square test (? < 0.05) showed the sig value of D-Dimer, PT, aPTT albumin with a severity level of 0.000; 0.000; 0.001; 0.001 while the value of Sig. with a mortality of 0.000; 0.047; 0.239; 0.022. The results of the multivariate logistic regression test with the degree of severity obtained the value of Sig. 0.000; 0.000; 0.021; 0.000 with a [PR] value of 16.7; 4.4; 2.7; 14.4. The results of the multivariate logistic regression test with mortality obtained the value of Sig. 0.000; 0.020; 0.273 with a [PR] value of 26.9; 2.8;1.6. There is a relationship between D-Dimer, PT, aPTT, and albumin with severity and mortality and can be used as a predictor of severity and mortality in COVID-19 patients.","PeriodicalId":31934,"journal":{"name":"Jurnal Teknologi Laboratorium","volume":"31 18 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-08-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139349093","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Transportation accounts for quite large errors up to 12% of the total errors. The error factor in this transportation activity comes from sending specimens that are too long and storage temperatures that are not right. Hematological examination is an examination that is often performed in various health services. The purpose of this study was to determine the stability of the hematological examination of blood samples transported for 1 hour and 2 hours at room temperature and cold temperatures. This study is a laboratory experimental study. Observations before treatment were controls, and treatments were carried out after 1 hour and 2 hours of transportation at room temperature and cold temperatures. This research was conducted at an average ambient temperature of 33.4ºC and humidity of 45%, room temperature of 27.8ºC and humidity of 43%, cold temperature of 4.5ºC and humidity of 52%, and average shock of 54.9. The results showed that the parameters of Hgb, PLT, WBC, and NEUT were stable for up to 2 hours in both room temperature and cold conditions. Meanwhile, the stability of LYMPH parameters is only capable at room temperature for 2 hours. Parameters Hct, RBC, MCV, MCHC, and MXD there are differences in the results of the examination at cold temperatures after 1 hour and 2 hours. However, the parameters of RBC, MCV, and MXD were stable for up to 1 hour at room temperature. We conclude that the hematological examination is stable for up to 1 hour with the recommended storage at room temperature. Only Hgb, PLT, WBC, and NEUT tests are stable for up to 2 hours in both room temperature and cold conditions.
{"title":"The effect of transportation on the stability of hematological examination","authors":"Gilang Nugraha, Notrisia Rachmayanti, Moch. Sahri, Maulana Bagas Syaifulloh, Salsabillah Karunia Sugiarto","doi":"10.29238/teknolabjournal.v12i1.308","DOIUrl":"https://doi.org/10.29238/teknolabjournal.v12i1.308","url":null,"abstract":"Transportation accounts for quite large errors up to 12% of the total errors. The error factor in this transportation activity comes from sending specimens that are too long and storage temperatures that are not right. Hematological examination is an examination that is often performed in various health services. The purpose of this study was to determine the stability of the hematological examination of blood samples transported for 1 hour and 2 hours at room temperature and cold temperatures. This study is a laboratory experimental study. Observations before treatment were controls, and treatments were carried out after 1 hour and 2 hours of transportation at room temperature and cold temperatures. This research was conducted at an average ambient temperature of 33.4ºC and humidity of 45%, room temperature of 27.8ºC and humidity of 43%, cold temperature of 4.5ºC and humidity of 52%, and average shock of 54.9. The results showed that the parameters of Hgb, PLT, WBC, and NEUT were stable for up to 2 hours in both room temperature and cold conditions. Meanwhile, the stability of LYMPH parameters is only capable at room temperature for 2 hours. Parameters Hct, RBC, MCV, MCHC, and MXD there are differences in the results of the examination at cold temperatures after 1 hour and 2 hours. However, the parameters of RBC, MCV, and MXD were stable for up to 1 hour at room temperature. We conclude that the hematological examination is stable for up to 1 hour with the recommended storage at room temperature. Only Hgb, PLT, WBC, and NEUT tests are stable for up to 2 hours in both room temperature and cold conditions.","PeriodicalId":31934,"journal":{"name":"Jurnal Teknologi Laboratorium","volume":"2011 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-08-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139350250","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-07-18DOI: 10.29238/teknolabjournal.v12i1.409
Indri Ari Ningtyas, Empi Irawan
Retinoblastoma is the commonest intraocular tumor in childhood. Management of retinoblastoma is quite complex depending on the stage, visual and globe saving, psychosocial factors, modality of treatment, and health infrastructure. We report a case of retinoblastoma in a child in a limited health facility without a pediatric oncologist or ophthalmology oncologist. A 22-month old boy came with complaints of leukocoria which his parents had known since the previous week. Ultrasonography of the eye shows calcification on the retina that supports Group E retinoblastoma. Bone marrow aspiration and MRI cannot be performed due to limited resources. Even though the parents only noticed the symptoms for a week and immediately brought to the ophthalmologist, the retinoblastoma stage was already in Group E. Enucleation and globe salvage were unavoidable. Enucleation was performed on the patient with histopathology finding the presence of Flexner-Wintersteiner rosette cells. After enucleation, he was referred to the oncologist for adjuvant chemotherapy. Early retinoblastoma detection is crucial in increasing the cure rate while maintaining children's vision.
视网膜母细胞瘤是儿童时期最常见的眼内肿瘤。视网膜母细胞瘤的治疗相当复杂，取决于肿瘤的分期、视力和眼球保护、社会心理因素、治疗方式以及医疗基础设施。我们报告了一例儿童视网膜母细胞瘤病例，这家医疗机构条件有限，没有儿科肿瘤专家或眼科肿瘤专家。一名 22 个月大的男童前来就诊，他的父母从上一周起就知道他有眼白。眼部超声波检查显示视网膜上有钙化，支持 E 组视网膜母细胞瘤。由于资源有限，无法进行骨髓穿刺和磁共振成像检查。尽管患者父母发现症状仅一周，并立即带患者到眼科医生处就诊，但视网膜母细胞瘤的分期已经是 E 组。对患者进行了去核手术，组织病理学检查发现存在 Flexner-Wintersteiner rosette 细胞。去核手术后，他被转到肿瘤科接受辅助化疗。早期发现视网膜母细胞瘤对提高治愈率和保护儿童视力至关重要。
{"title":"Enucleation for retinoblastoma in a 22-month-old boy","authors":"Indri Ari Ningtyas, Empi Irawan","doi":"10.29238/teknolabjournal.v12i1.409","DOIUrl":"https://doi.org/10.29238/teknolabjournal.v12i1.409","url":null,"abstract":"Retinoblastoma is the commonest intraocular tumor in childhood. Management of retinoblastoma is quite complex depending on the stage, visual and globe saving, psychosocial factors, modality of treatment, and health infrastructure. We report a case of retinoblastoma in a child in a limited health facility without a pediatric oncologist or ophthalmology oncologist. A 22-month old boy came with complaints of leukocoria which his parents had known since the previous week. Ultrasonography of the eye shows calcification on the retina that supports Group E retinoblastoma. Bone marrow aspiration and MRI cannot be performed due to limited resources. Even though the parents only noticed the symptoms for a week and immediately brought to the ophthalmologist, the retinoblastoma stage was already in Group E. Enucleation and globe salvage were unavoidable. Enucleation was performed on the patient with histopathology finding the presence of Flexner-Wintersteiner rosette cells. After enucleation, he was referred to the oncologist for adjuvant chemotherapy. Early retinoblastoma detection is crucial in increasing the cure rate while maintaining children's vision.","PeriodicalId":31934,"journal":{"name":"Jurnal Teknologi Laboratorium","volume":"42 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-07-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139358259","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-06-21DOI: 10.29238/teknolabjournal.v12i1.392
Gela Setya Ayu Putri, Andri Sukeksi
Platelet aggregation test is important in evaluation platelet function. Inductors are substances that stimulate the aggregation process. The use of ADP (adenosine diphosphate as an inductor has the disadvantage of being costly and difficult to obtain. Aloe vera is a plant from Africa which has been widely used for wound healing, therefore has the potential as an alternative inductor agent. This study aims to elucidate the effectiveness of Aloe vera gel as a platelet inductor compared to ADP reagent with the Velaskar method. This research started with the preparation of fresh Aloe vera to obtain Aloe vera gel. Then, venous blood was taken from 16 participants for platelet aggregation test. Platelet aggregation test was divided into two groups, namely ADP group (addition of ADP) and Aloe vera group (addition of aloe vera gel). The last step was analyze the results of platelet aggregation and statistically using the Independent Sample T-test. The average of percentage platelet aggregation with ADP and Aloe vera gel was 88.2 ± 5.6% and 85.7 ± 5.4%, respectively. Independent sample T-test analysis showed no significant difference between the percentage of platelet aggregation with ADP and Aloe vera gel. In conclusion, Aloe vera gel has the potential as an alternate platelet inductor. Some of the benefits of aloe vera gel are the ability to induce platelets as well as ADP, the application does not affect the morphology of erythrocytes, economical and practical (does not require elaborate preparation). Although the disadvantage is pure Aloe vera gel includes several components that able affect the performance of Aloe vera gel as an inductor.
{"title":"POTENTIAL OF ALOE VERA GEL AS AN ALTERNATIVE INDUCTOR IN PLATELET AGGREGATION TEST","authors":"Gela Setya Ayu Putri, Andri Sukeksi","doi":"10.29238/teknolabjournal.v12i1.392","DOIUrl":"https://doi.org/10.29238/teknolabjournal.v12i1.392","url":null,"abstract":"Platelet aggregation test is important in evaluation platelet function. Inductors are substances that stimulate the aggregation process. The use of ADP (adenosine diphosphate as an inductor has the disadvantage of being costly and difficult to obtain. Aloe vera is a plant from Africa which has been widely used for wound healing, therefore has the potential as an alternative inductor agent. This study aims to elucidate the effectiveness of Aloe vera gel as a platelet inductor compared to ADP reagent with the Velaskar method. This research started with the preparation of fresh Aloe vera to obtain Aloe vera gel. Then, venous blood was taken from 16 participants for platelet aggregation test. Platelet aggregation test was divided into two groups, namely ADP group (addition of ADP) and Aloe vera group (addition of aloe vera gel). The last step was analyze the results of platelet aggregation and statistically using the Independent Sample T-test. The average of percentage platelet aggregation with ADP and Aloe vera gel was 88.2 ± 5.6% and 85.7 ± 5.4%, respectively. Independent sample T-test analysis showed no significant difference between the percentage of platelet aggregation with ADP and Aloe vera gel. In conclusion, Aloe vera gel has the potential as an alternate platelet inductor. Some of the benefits of aloe vera gel are the ability to induce platelets as well as ADP, the application does not affect the morphology of erythrocytes, economical and practical (does not require elaborate preparation). Although the disadvantage is pure Aloe vera gel includes several components that able affect the performance of Aloe vera gel as an inductor.","PeriodicalId":31934,"journal":{"name":"Jurnal Teknologi Laboratorium","volume":"60 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-06-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139369144","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-06-01DOI: 10.29238/teknolabjournal.v12i1.393
Rut Indah Susilo, Ali Napiah Nasution, M. Mutia
Acne vulgaris is a chronic inflammation of the pilosebaceous unit with various clinical manifestations caused by bacterial colonization of Propionibacterium acnes. Various studies have been performed to improve the modality of acne vulgaris therapy with natural product, such as leaves of Mahkota Dewa (Phaleria macrocarpa). This experimental study aims to investigate the effect of the Mahkota Dewa leaves extract against P. acnes by using Disc Diffusion method and Inhibition of Biofilm formation. Research results showed that the Total Flavonoid and Tannin content were 953.10 mg QE/gr DW and 42.67 mg TAE/gr DW, respectively. The Mahkota Dewa leaves extract had a significant antibacterial effect on P. acnes bacteria by using disc diffusion method (p < 0.05), with the widest inhibition zone diameter found at a concentration of 90 ppm (19.20 mm) and the narrowest was 30 ppm (14.20 ppm). Mahkota Dewa leaves extract also significantly inhibited the formation of P. acnes biofilms, where the highest inhibition activity was found at a concentration of 90 ppm (71.58 ± 1.49%) and the lowest was found at a concentration of 30 ppm (58.24 ± 2.52%). Mahkota Dewa leaves extract has showed an antibacterial effect against P. acnes and promise a potential use of acne vulgaris therapy.
{"title":"Effectiveness of mahkota dewa leaves extract as a biofilm inhibitor of propionibacterium acnes growth","authors":"Rut Indah Susilo, Ali Napiah Nasution, M. Mutia","doi":"10.29238/teknolabjournal.v12i1.393","DOIUrl":"https://doi.org/10.29238/teknolabjournal.v12i1.393","url":null,"abstract":"Acne vulgaris is a chronic inflammation of the pilosebaceous unit with various clinical manifestations caused by bacterial colonization of Propionibacterium acnes. Various studies have been performed to improve the modality of acne vulgaris therapy with natural product, such as leaves of Mahkota Dewa (Phaleria macrocarpa). This experimental study aims to investigate the effect of the Mahkota Dewa leaves extract against P. acnes by using Disc Diffusion method and Inhibition of Biofilm formation. Research results showed that the Total Flavonoid and Tannin content were 953.10 mg QE/gr DW and 42.67 mg TAE/gr DW, respectively. The Mahkota Dewa leaves extract had a significant antibacterial effect on P. acnes bacteria by using disc diffusion method (p < 0.05), with the widest inhibition zone diameter found at a concentration of 90 ppm (19.20 mm) and the narrowest was 30 ppm (14.20 ppm). Mahkota Dewa leaves extract also significantly inhibited the formation of P. acnes biofilms, where the highest inhibition activity was found at a concentration of 90 ppm (71.58 ± 1.49%) and the lowest was found at a concentration of 30 ppm (58.24 ± 2.52%). Mahkota Dewa leaves extract has showed an antibacterial effect against P. acnes and promise a potential use of acne vulgaris therapy.","PeriodicalId":31934,"journal":{"name":"Jurnal Teknologi Laboratorium","volume":"300 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-06-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139371715","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
Pub Date : 2023-05-30DOI: 10.29238/teknolabjournal.v12i1.406
M. Gondokesumo, K. Budipramana, Putu Dea Angelita Putri, Ni Putu Diah Nopitasari, Martanty Aditya, Liza Yudistira Yusan
Parkinson’s disease is a condition that affects the central nervous system in the brain and is brought on by a lack of dopamine. Uncontrollable tremors, uncoordinated movement, and stiffness characterize Parkinson’s disease. Until now, the medication for Parkinson’s disease is limited to relieve the symptoms and maintain the quality of life; thus, the progression of the disease can be delayed. In order to search for alternative therapy from herbs, Kluwih (Artocarpus camansi) has been used traditionally to relieve convulsants. This research aims to observe 96% ethanol extract of A. camansi leaves in dopamine and locomotor activity in adult male and female zebrafish (Dario rerio). The A. camansi extract concentration was 2.5; 5; 7.5; and 10 mg/ml for 28 days. Zebrafish locomotion was observed for 5 minutes on days 0; 7; 14; 21; and 28. ELISA measured the observations of dopamine after 28 days. The 96% ethanol extract of A. camansi leaves at 5 mg/ml can increase dopamine levels after induced with rotenone, but the dopamine level decreased at 7,5 and 10 mg/ml. The maximal concentration to increase locomotor activity is also at 5 mg/ml, along with dopamine concentration. Our findings revealed that 5 mg/ml of 96% ethanol extract of A. camansi leaves was the optimal dosage to stimulate dopamine release and enhance locomotor activity.
{"title":"Keluwih (Artocarpus camansi) extract effects in zebrafish models of Parkinson’s disease","authors":"M. Gondokesumo, K. Budipramana, Putu Dea Angelita Putri, Ni Putu Diah Nopitasari, Martanty Aditya, Liza Yudistira Yusan","doi":"10.29238/teknolabjournal.v12i1.406","DOIUrl":"https://doi.org/10.29238/teknolabjournal.v12i1.406","url":null,"abstract":"Parkinson’s disease is a condition that affects the central nervous system in the brain and is brought on by a lack of dopamine. Uncontrollable tremors, uncoordinated movement, and stiffness characterize Parkinson’s disease. Until now, the medication for Parkinson’s disease is limited to relieve the symptoms and maintain the quality of life; thus, the progression of the disease can be delayed. In order to search for alternative therapy from herbs, Kluwih (Artocarpus camansi) has been used traditionally to relieve convulsants. This research aims to observe 96% ethanol extract of A. camansi leaves in dopamine and locomotor activity in adult male and female zebrafish (Dario rerio). The A. camansi extract concentration was 2.5; 5; 7.5; and 10 mg/ml for 28 days. Zebrafish locomotion was observed for 5 minutes on days 0; 7; 14; 21; and 28. ELISA measured the observations of dopamine after 28 days. The 96% ethanol extract of A. camansi leaves at 5 mg/ml can increase dopamine levels after induced with rotenone, but the dopamine level decreased at 7,5 and 10 mg/ml. The maximal concentration to increase locomotor activity is also at 5 mg/ml, along with dopamine concentration. Our findings revealed that 5 mg/ml of 96% ethanol extract of A. camansi leaves was the optimal dosage to stimulate dopamine release and enhance locomotor activity.","PeriodicalId":31934,"journal":{"name":"Jurnal Teknologi Laboratorium","volume":"32 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2023-05-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139372675","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
One of the complications of Diabetes Mellitus (DM) is damage to the kidneys. Using L. plantarum, it can reduce glucose levels so that it is also possible to repair damaged kidney structures. The process of repairing the kidney structure by L plantarum can be seen microscopically in the glomerulus with Alcian Blue staining (AB). The purpose of the study was to determine the differences in the microscopic results of DM rat kidneys after treatment of L. plantarum with AB staining. The study sample used the kidneys of DM mice. The kidney of DM mice that were given L. plantarum at a dose of 1.0 ml/mice in each treatment, T1 (dose 1×1 times a day), T2 (dose 1×2 times a day), T3 (dose 1×3 times a day), then performed AB staining. The results of cytoplasmic cells in the glomerulus in negative controls/sick mice are stained dark blue/concentrated (strong intensity) with a score of 4, normal controls/healthy mice are not stained blue (negative) with a score of 1. T1 is a stained light blue score of 3, T2 is a stained light blue score of 3, and T3 is a colored pale blue score of 2. Based on these results, it was found that the color intensity of the AB kidney treatment of DM rats was the best in reducing glucose residues.
{"title":"Alcian blue as a kidney staining in diabetic mice","authors":"Sri Sinto Dewi, Suci Indah Astuti, Fitri Nuroini, Aprilia Indra Kartika","doi":"10.29238/teknolabjournal.v11i2.386","DOIUrl":"https://doi.org/10.29238/teknolabjournal.v11i2.386","url":null,"abstract":"One of the complications of Diabetes Mellitus (DM) is damage to the kidneys. Using L. plantarum, it can reduce glucose levels so that it is also possible to repair damaged kidney structures. The process of repairing the kidney structure by L plantarum can be seen microscopically in the glomerulus with Alcian Blue staining (AB). The purpose of the study was to determine the differences in the microscopic results of DM rat kidneys after treatment of L. plantarum with AB staining. The study sample used the kidneys of DM mice. The kidney of DM mice that were given L. plantarum at a dose of 1.0 ml/mice in each treatment, T1 (dose 1×1 times a day), T2 (dose 1×2 times a day), T3 (dose 1×3 times a day), then performed AB staining. The results of cytoplasmic cells in the glomerulus in negative controls/sick mice are stained dark blue/concentrated (strong intensity) with a score of 4, normal controls/healthy mice are not stained blue (negative) with a score of 1. T1 is a stained light blue score of 3, T2 is a stained light blue score of 3, and T3 is a colored pale blue score of 2. Based on these results, it was found that the color intensity of the AB kidney treatment of DM rats was the best in reducing glucose residues.","PeriodicalId":31934,"journal":{"name":"Jurnal Teknologi Laboratorium","volume":" ","pages":""},"PeriodicalIF":0.0,"publicationDate":"2022-12-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41983278","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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