Identification of the potential function of circRNA in hypertrophic cardiomyopathy based on mutual RNA-RNA and RNA-RBP relationships shown by microarray data

Abstract

The pathogenesis of hypertrophic cardiomyopathy (HCM) is very complicated, particularly regarding the role of circular RNA (circRNA). This research pays special attention to the relationships of the circRNA-mediated network, including RNA-RNA relationships and RNA-RNA binding protein (RNA-RBP) relationships. We use the parameter framework technology proposed in this paper to screen differentially expressed circRNA, messenger RNA (mRNA), and microRNA (miRNA) from the expression profile of samples related to HCM. And 31 pairs of circRNA and mRNA relationship pairs were extracted, combined with the miRNA targeting database; 145 miRNA-mRNA relationship pairs were extracted; 268 circRNA-mRNA-miRNA triads were established through the common mRNA in the 2 types of relationship pairs. Thus, 268 circRNA-miRNA regulatory relationships were deduced and 30 circRNA-RBP relationship pairs were analyzed at the protein level. On this basis, a circRNA-mediated regulatory network corresponding to the two levels of RNA-RNA and RNA-RBP was established. And then the roles of circRNA in HCM were analyzed through circRNA-mRNA, circRNA-miRNA, and circRNA-RBP, and the possible role in disease development was inferred.