Chemical composition and antimicrobial activity of leave extract of Ocimum lamiifolium (Damakese) as a treatment for urinary tract infection

Abstract

Abstract Natural products obtained from aromatic plants are considered to be one of the potential sources for the screening of antimicrobial, antioxidant, and free radical scavenging agents. Ocimum Lamiifolium (Damakese) is one of the well-known celebrated and most widely used home remedies. The shade dried leaves of Ocimum Lamiifolium were subjected to hydrodistillation in a Clevenger-type apparatus to extract its essential oil. The yield of hydrodistilled colorless, pungent odor oil was found to be 0.22% (v/w). The chemical composition of the oil was analyzed by GC-MS method. Ten constituents were identified representing 93.56% of the total oil. The main constituents of the oil were found to be Linalool (28.52%) and 1-octen-3-yl-npropionate (20.82%) followed by 3,7,11-trimethyl-(E,E)-2,6,10-dodecatrienal (12.14%). The yield of crude extract of Ocimum Lamiifolium was 0.28% (w/w). Phytochemical screening revealed the presence of terpenoids, flavonoids, tannin, saponins and steroids. The in vitro antibacterial activity of Ocimum Lamiifolium leaf ethanol extract and essential oil was studied by using disc diffusion method. The oil exhibited significant zone of inhibitions against to E. coli bacterial strains.