The past, present, and future of artificial zinc finger proteins: design strategies and chemical and biological applications

IF 2.7 3区化学 Q3 BIOCHEMISTRY & MOLECULAR BIOLOGY JBIC Journal of Biological Inorganic Chemistry Pub Date : 2023-02-07 DOI:10.1007/s00775-023-01991-6

Shigeru Negi, Miki Imanishi, Mami Hamori, Yuka Kawahara-Nakagawa, Wataru Nomura, Kanae Kishi, Nobuhito Shibata, Yukio Sugiura

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引用次数: 2

Abstract

Zinc finger proteins are abundant in the human proteome and are responsible for a variety of functions. The domains that constitute zinc finger proteins are compact spherical structures, each comprising approximately 30 amino acid residues, but they also have precise molecular factor functions: zinc binding and DNA recognition. Due to the biological importance of zinc finger proteins and their unique structural and functional properties, many artificial zinc finger proteins have been created and are expected to improve their functions and biological applications. In this study, we review previous studies on the redesign and application of artificial zinc finger proteins, focusing on the experimental results obtained by our research group. In addition, we systematically review various design strategies used to construct artificial zinc finger proteins and discuss in detail their potential biological applications, including gene editing. This review will provide relevant information to researchers involved or interested in the field of artificial zinc finger proteins as a potential new treatment for various diseases.

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人造锌指蛋白的过去、现在和未来:设计策略和化学和生物应用

锌指蛋白在人类蛋白质组中含量丰富，具有多种功能。构成锌指蛋白的结构域是紧凑的球形结构，每个结构域由大约30个氨基酸残基组成，但它们也具有精确的分子因子功能:锌结合和DNA识别。由于锌指蛋白的生物学重要性和其独特的结构和功能特性，许多人造锌指蛋白已经被创造出来，并有望提高其功能和生物学应用。在本研究中，我们回顾了以往关于人造锌指蛋白的再设计和应用的研究，重点介绍了本课课组的实验结果。此外，我们系统地回顾了用于构建人造锌指蛋白的各种设计策略，并详细讨论了其潜在的生物学应用，包括基因编辑。本文将对人工锌指蛋白作为一种潜在的治疗多种疾病的新方法提供相关信息。

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来源期刊

JBIC Journal of Biological Inorganic Chemistry 化学-生化与分子生物学

CiteScore

5.90

自引率

3.30%

发文量

审稿时长

3 months

期刊介绍： Biological inorganic chemistry is a growing field of science that embraces the principles of biology and inorganic chemistry and impacts other fields ranging from medicine to the environment. JBIC (Journal of Biological Inorganic Chemistry) seeks to promote this field internationally. The Journal is primarily concerned with advances in understanding the role of metal ions within a biological matrix—be it a protein, DNA/RNA, or a cell, as well as appropriate model studies. Manuscripts describing high-quality original research on the above topics in English are invited for submission to this Journal. The Journal publishes original articles, minireviews, and commentaries on debated issues.