Evaluation of PPP2R5C gene expression in Iranian patients with B-Acute lymphoblastic leukemia and its association with clinical and laboratory findings

Abstract

Introduction: PPP2R5C is one of the regulatory B subunits of protein phosphatase 2A (PP2A), which is a tumor suppressor. PPP2R5C plays a critical role in cell proliferation, differentiation, and transformation. Considering these vital functions, we investigate the gene expression in Iranian patients with B-Acute Lymphoblastic Leukemia (B-ALL) and its association with clinical and laboratory finding. Materials and methods: In this case-control study, peripheral blood samples were collected from 60 B-ALL patients and 30 healthy controls. PPP2R5C expression levels were determined by Real-time PCR. After calculation of CT for target and control genes, we calculated ΔCT. Finally we compared the PPP2R5C expression levels in patients with control group. Results: Significantly higher expression of PPP2R5C was found in the B-ALL patients (2.15±2. 50) compared with control group. There was no correlation between PPP2R5C expression and clinical and laboratory findings and FAB (French-American-British) subtype of patients. Conclusion: we demonstrated PPP2R5C overexpression in B-ALL patients. Although there was no significant correlation between PPP2R5C expression, clinical and laboratory finding and also with FAB subtypes of patients.