Effects of combined cannabidiol (CBD) and hops (Humulus lupulus) terpene extract treatment on RAW 264.7 macrophage viability and inflammatory markers

IF 4.8 3区 化学 Q1 CHEMISTRY, MEDICINAL Natural Products and Bioprospecting Pub Date : 2023-06-07 DOI:10.1007/s13659-023-00382-3
Inga Dammann, Claudia Keil, Iris Hardewig, Elżbieta Skrzydlewska, Michał Biernacki, Hajo Haase
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引用次数: 1

Abstract

This study investigates the potential of cannabidiol (CBD), one major cannabinoid of the plant Cannabis sativa, alone and in combination with a terpene-enriched extract from Humulus lupulus (“Hops 1”), on the LPS-response of RAW 264.7 macrophages as an established in vitro model of inflammation. With the present study, we could support earlier findings of the anti-inflammatory potential of CBD, which showed a dose-dependent [0–5 µM] reduction in nitric oxide and tumor necrosis factor-alpha (TNF-α) released by LPS-stimulated RAW 264.7 macrophages. Moreover, we observed an additive anti-inflammatory effect after combined CBD [5 µM] and hops extract [40 µg/mL] treatment. The combination of CBD and Hops 1 showed effects in LPS-stimulated RAW 264.7 cells superior to the single substance treatments and akin to the control hydrocortisone. Furthermore, cellular CBD uptake increased dose-dependently in the presence of terpenes from Hops 1 extract. The anti-inflammatory effect of CBD and its cellular uptake positively correlated with terpene concentration, as indicated by comparison with a hemp extract containing both CBD and terpenes. These findings may contribute to the postulations for the so-called “entourage effect” between cannabinoids and terpenes and support the potential of CBD combined with phytomolecules from a non-cannabinoid source, such as hops, for the treatment of inflammatory diseases.

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大麻二酚(CBD)和啤酒花(Humulus lupulus)萜烯提取物联合处理对RAW 264.7巨噬细胞活力和炎症标志物的影响
作为一种已建立的体外炎症模型,本研究探讨了大麻二酚(cannabidiol, CBD)作为植物大麻的主要大麻素之一,单独使用和与葎草(Humulus lupulus)富含萜烯的提取物(“Hops 1”)联合使用对RAW 264.7巨噬细胞lps反应的影响。通过本研究,我们可以支持早期关于CBD抗炎潜力的发现,该发现显示lps刺激的RAW 264.7巨噬细胞释放的一氧化氮和肿瘤坏死因子-α (TNF-α)呈剂量依赖性[0-5µM]降低。此外,我们还观察到CBD[5µM]和啤酒花提取物[40µg/mL]联合处理后的附加抗炎作用。CBD和啤酒花1联合使用对lps刺激的RAW 264.7细胞的影响优于单一物质处理,与对照氢化可的松相似。此外,在啤酒花1提取物中存在萜烯的情况下,细胞对CBD的摄取呈剂量依赖性增加。通过与同时含有CBD和萜烯的大麻提取物进行比较,发现CBD的抗炎作用及其细胞摄取与萜烯浓度呈正相关。这些发现可能有助于大麻素和萜烯之间所谓的“随行效应”的假设,并支持CBD与来自非大麻素来源的植物分子(如啤酒花)结合治疗炎症性疾病的潜力。
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来源期刊
Natural Products and Bioprospecting
Natural Products and Bioprospecting CHEMISTRY, MEDICINAL-
CiteScore
8.30
自引率
2.10%
发文量
39
审稿时长
13 weeks
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